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Literature summary extracted from

  • De Oliveira, M.E.; Battastini, O.A.M.; Meirelles, M.N.; Moreira, M.C.; Dias, R.D.; Sarkis, J.J.
    Characterization and localisation of an ATP diphosphohydrolase activity (EC 3.6.1.5) in sarcolemmal membrane from rat heart (1997), Mol. Cell. Biochem., 170, 115-123.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.5 EGTA
-
 Rattus norvegicus
3.6.1.5 HgCl
-
 Rattus norvegicus
3.6.1.5 additional information no inhibition by lanthanum; no inhibition by levamisole; no inhibition by N-ethylmaleimide; no inhibition by oligomycin; no inhibition by orthovanadate; no inhibition by P1,P5-di(adenosine-5'-)pentanphospate Rattus norvegicus
3.6.1.5 NaN3 above 10 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.5 0.062
-
 ATP
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.5 membrane membrane-bound Rattus norvegicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.5 Ca2+
-
 Rattus norvegicus
3.6.1.5 Co2+ lower activation than Ca2+, Mg2+, Mn2+ Rattus norvegicus
3.6.1.5 Mg2+ either Mg2+ or Ca2+ required for activity Rattus norvegicus
3.6.1.5 Mn2+ lower activation than Ca2+ and Mg2+ Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.5 ADP + H2O Rattus norvegicus
-
 AMP + phosphate
-
 ?
3.6.1.5 ATP + 2 H2O Rattus norvegicus
-
 AMP + 2 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.5 Rattus norvegicus
-
 rat
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.5 heart ventricle, sarcolemma Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.5 0.27
-
 ADP, 1.5 mM Ca2+, sarcolemmal fraction Rattus norvegicus
3.6.1.5 1.2
-
 ATP, 1.5 mM Ca2+, sarcolemmal fraction Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.5 ADP + H2O
-
 Rattus norvegicus AMP + phosphate
-
 ?
3.6.1.5 ATP + 2 H2O
-
 Rattus norvegicus AMP + 2 phosphate
-
 ?
3.6.1.5 additional information neglible hydrolysis of AMP Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.5 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.5 7.5 8
-
 Rattus norvegicus