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Literature summary extracted from

  • Mans, B.J.; Gaspar, A.I.; Louw, A.I.; Neitz, A.W.H.
    Purification and characterization of apyrase from the tick, Ornithodoros savignyi (1998), Comp. Biochem. Physiol. B, 120, 617-624.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.5 DTT
-
 Ornithodoros savignyi
3.6.1.5 EDTA activity not restored with 5 mM Ca2+ or Mg2+ for periods up to 24h Ornithodoros savignyi
3.6.1.5 EGTA
-
 Ornithodoros savignyi
3.6.1.5 HgCl
-
 Ornithodoros savignyi
3.6.1.5 additional information no inhibition by azide; no inhibition by orthovanadate; no inhibition by ouabain Ornithodoros savignyi
3.6.1.5 NaF
-
 Ornithodoros savignyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.5 0.76
-
 ATP
-
 Ornithodoros savignyi
3.6.1.5 1.04
-
 ADP
-
 Ornithodoros savignyi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.5 Ca2+ only ADP hydrolysed in the presence of Ca2+ Ornithodoros savignyi
3.6.1.5 Mg2+
-
 Ornithodoros savignyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.5 67000
-
 SDS-PAGE Ornithodoros savignyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.5 ADP + H2O Ornithodoros savignyi
-
 AMP + phosphate
-
 ?
3.6.1.5 ATP + 2 H2O Ornithodoros savignyi
-
 AMP + 2 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.5 Ornithodoros savignyi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.5
-
 Ornithodoros savignyi

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.5 salivary gland
-
 Ornithodoros savignyi
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.5 200
-
 MgADP- Ornithodoros savignyi
3.6.1.5 280
-
 CaADP- Ornithodoros savignyi
3.6.1.5 360
-
 MgADP- Ornithodoros savignyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.5 ADP + H2O
-
 Ornithodoros savignyi AMP + phosphate
-
 ?
3.6.1.5 ATP + 2 H2O
-
 Ornithodoros savignyi AMP + 2 phosphate
-
 ?
3.6.1.5 additional information neglible hydrolysis of AMP Ornithodoros savignyi ?
-
 ?
3.6.1.5 UDP + H2O
-
 Ornithodoros savignyi UMP + phosphate
-
 ?
3.6.1.5 UTP + 2 H2O
-
 Ornithodoros savignyi UMP + 2 phosphate
-
 ?