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Literature summary extracted from

  • Choi, S.Y.; Esaki, N.; Yoshimura, T.; Soda, K.
    Overproduction of glutamate racemase of Pediococcus pentosaceus in Escherchia coli clone cells and its purification (1991), Protein Expr. Purif., 2, 90-93.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.3 expression in Escherichia coli Pediococcus pentosaceus

General Stability

EC Number General Stability Organism
5.1.1.3 repeated thawing and melting of the enzyme causes inactivation Pediococcus pentosaceus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.3 additional information
-
 additional information
-
 Pediococcus pentosaceus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.1.3 29000
-
 gel filtration Pediococcus pentosaceus
5.1.1.3 29140
-
 calculation from nucleotide sequence Pediococcus pentosaceus

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.3 Pediococcus pentosaceus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.3
-
 Pediococcus pentosaceus

Storage Stability

EC Number Storage Stability Organism
5.1.1.3 -20°C, 50 mM Tris-HCl, pH 7.5, 1 mM DL-GLu, 10% glycerol, 0.1% 2-mercaptoethanol, 0.1 mM phenylmethanesulfonyl fluoride, stable for several months Pediococcus pentosaceus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.3 D-Glu
-
 Pediococcus pentosaceus L-Glu
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.1.3 monomer 1 * 29000, SDS-PAGE Pediococcus pentosaceus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.1.1.3 6.5 8.5 25°C, 15 min, stable Pediococcus pentosaceus