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Literature summary extracted from

  • Yanagisawa, K.; Resnick, D.; Abeijon, C.; Robbins, P.W.; Hirschberg, C.B.
    A guanosine diphosphatase enriched in Golgi vesicles of Saccharomyces cerevisiae. Purification and characterization (1990), J. Biol. Chem., 265, 19351-19355.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.42 0.1
-
 GDP
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.42 Golgi lumen
-
 Saccharomyces cerevisiae 5796
-
3.6.1.42 Golgi lumen
-
 Schizosaccharomyces pombe 5796
-
3.6.1.42 Golgi membrane
-
 Saccharomyces cerevisiae 139
-
3.6.1.42 Golgi membrane
-
 Schizosaccharomyces pombe 139
-
3.6.1.42 Golgi vesicle Golgi-like vesicles Saccharomyces cerevisiae
-
-
3.6.1.42 Golgi vesicle Golgi-like vesicles Schizosaccharomyces pombe
-
-
3.6.1.42 membrane
-
 Saccharomyces cerevisiae 16020
-
3.6.1.42 membrane
-
 Schizosaccharomyces pombe 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.42 Ca2+ required for maximal activity Saccharomyces cerevisiae
3.6.1.42 divalent cations required Saccharomyces cerevisiae
3.6.1.42 Fe2+ less than 10% activation Saccharomyces cerevisiae
3.6.1.42 Mg2+ activation Saccharomyces cerevisiae
3.6.1.42 Mn2+ activation, enzyme with Mn2+ is 60-95% as active as compared with Ca2+ Saccharomyces cerevisiae
3.6.1.42 Mn2+ in the presence of 10 mM Mn2+: 3fold activation of its activity toward UDP, but not GDP, compared to activation by 10 mM Ca2+ Saccharomyces cerevisiae
3.6.1.42 additional information
-
 Saccharomyces cerevisiae
3.6.1.42 Ni2+ less than 10% activation Saccharomyces cerevisiae
3.6.1.42 Zn2+ less than 10% activation Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.42 47000
-
 deglycosylated GDPase, result of proteolytic cleavage of the 55000 Da enzyme during purification, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.42 GDP + H2O Saccharomyces cerevisiae GDP is a reaction product following outer-chain mannosylation of luminal proteins and inhibitor of mannosyltransferases. It is hypothesized that GMP then returns to the cytosol. GDPase presumably serves as an essential function in processing of yeast glycoproteins 5'-GMP + phosphate
-
 ?
3.6.1.42 GDP + H2O Saccharomyces cerevisiae GDP is known to be an inhibitor of mannosylation reactions in the Golgi lumen, removal of GDP by conversion to GMP, the latter not being an inhibitor of these reactions, would allow mannosylation to proceed 5'-GMP + phosphate
-
 ?
3.6.1.42 additional information Saccharomyces cerevisiae role in mannosylation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.42 Saccharomyces cerevisiae
-
-
-
3.6.1.42 Schizosaccharomyces pombe
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.6.1.42 glycoprotein
-
 Saccharomyces cerevisiae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.42
-
 Saccharomyces cerevisiae
3.6.1.42 partial Schizosaccharomyces pombe

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.42 3080
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.42 GDP + H2O best substrate Saccharomyces cerevisiae 5'-GMP + phosphate
-
 ?
3.6.1.42 GDP + H2O best substrate Schizosaccharomyces pombe 5'-GMP + phosphate
-
 ?
3.6.1.42 GDP + H2O highly specific for GDP Saccharomyces cerevisiae 5'-GMP + phosphate
-
 ?
3.6.1.42 GDP + H2O GDP is a reaction product following outer-chain mannosylation of luminal proteins and inhibitor of mannosyltransferases. It is hypothesized that GMP then returns to the cytosol. GDPase presumably serves as an essential function in processing of yeast glycoproteins Saccharomyces cerevisiae 5'-GMP + phosphate
-
 ?
3.6.1.42 GDP + H2O GDP is known to be an inhibitor of mannosylation reactions in the Golgi lumen, removal of GDP by conversion to GMP, the latter not being an inhibitor of these reactions, would allow mannosylation to proceed Saccharomyces cerevisiae 5'-GMP + phosphate
-
 ?
3.6.1.42 additional information substrate specificity Saccharomyces cerevisiae ?
-
 ?
3.6.1.42 additional information substrate specificity Schizosaccharomyces pombe ?
-
 ?
3.6.1.42 additional information less than 2% of maximum activity with other nucleoside di-, mono-, and triphosphates Saccharomyces cerevisiae ?
-
 ?
3.6.1.42 additional information less than 2% of maximum activity with other nucleoside di-, mono-, and triphosphates Schizosaccharomyces pombe ?
-
 ?
3.6.1.42 additional information role in mannosylation Saccharomyces cerevisiae ?
-
 ?
3.6.1.42 UDP + H2O about 10% as active with UDP compared to GDP Saccharomyces cerevisiae UMP + phosphate
-
 ?
3.6.1.42 UDP + H2O about 10% as active with UDP compared to GDP Schizosaccharomyces pombe UMP + phosphate
-
 ?
3.6.1.42 UDP + H2O in the presence of 10 mM Mn2+: 3fold activation of its activity toward UDP, but not GDP, compared to activation by 10 mM Ca2+ Saccharomyces cerevisiae UMP + phosphate
-
 ?
3.6.1.42 UDP + H2O in the presence of 10 mM Mn2+: 3fold activation of its activity toward UDP, but not GDP, compared to activation by 10 mM Ca2+ Schizosaccharomyces pombe UMP + phosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.42 3830
-
 GDP
-
 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.42 7.4 8.2 broad pH optimum Saccharomyces cerevisiae