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Literature summary extracted from

  • Spahr, P.F.; Gesteland, R.F.
    Purification and properties of a new enzyme from Escherichia coli: nucleoside phosphacyl hydrolase (1970), Eur. J. Biochem., 12, 270-284.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.24 Li2SO4 160% of standard activity at 0.75 M Escherichia coli
3.6.1.24 NH4Cl 180% of standard activity at 1 M Escherichia coli

General Stability

EC Number General Stability Organism
3.6.1.24 inactivation by repeated freezing and thawing Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.24 alanyl adenylate competitive inhibition of other substrates Escherichia coli
3.6.1.24 aminoalkyl adenylate strongest inhibitor, competitive inhibition of other substrates Escherichia coli
3.6.1.24 AMP competitive Escherichia coli
3.6.1.24 KI 50% loss of activity at 1 M Escherichia coli
3.6.1.24 lysyl adenylate competitive inhibition of other substrates Escherichia coli
3.6.1.24 Methionyl adenylate competitive inhibition of other substrates Escherichia coli
3.6.1.24 SDS complete loss of activity at 6 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.24 0.056
-
 adenosine 5'-p-nitrophenylphosphate
-
 Escherichia coli
3.6.1.24 0.11
-
 guanosine 5'-p-nitrophenylphosphate
-
 Escherichia coli
3.6.1.24 0.65
-
 uridine 5'-p-nitrophenylphosphate
-
 Escherichia coli
3.6.1.24 4
-
 cytidine 5'-p-nitrophenylphosphate
-
 Escherichia coli
3.6.1.24 770
-
 thymidine 5'-p-nitrophenylphosphate
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.24 additional information not periplasm Escherichia coli
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.24 additional information no metal ions required Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.24 23000
-
 sedimentation equilibrium method, gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.24 p-nitrophenyl 5'-nucleotides + H2O Escherichia coli hydrolysis A,C,G,U,T nucleotides p-nitrophenol + nucleoside phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.24 Escherichia coli
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.6.1.24 stable towards reducing agents Escherichia coli

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.24
-
 Escherichia coli

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.6.1.24
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.24 0.0018
-
 crude extract Escherichia coli
3.6.1.24 6670
-
 purified enzyme Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.6.1.24 -20°C, can be stored for at least one year Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.24 acetyl adenylate + H2O
-
 Escherichia coli ?
-
 ?
3.6.1.24 adenosine 5'-p-nitrophenylphosphate + H2O
-
 Escherichia coli p-nitrophenol + AMP
-
 ?
3.6.1.24 cytidine 5'-p-nitrophenylphosphate + H2O
-
 Escherichia coli p-nitrophenol + CMP
-
 ?
3.6.1.24 guanosine 5'-p-nitrophenylphosphate + H2O
-
 Escherichia coli p-nitrophenol + GMP
-
 ?
3.6.1.24 methionyl adenylate + H2O
-
 Escherichia coli ?
-
 ?
3.6.1.24 p-nitrophenyl 5'-nucleotides + H2O hydrolysis A,C,G,U,T nucleotides Escherichia coli p-nitrophenol + nucleoside phosphate
-
 ?
3.6.1.24 thymidine 5'-p-nitrophenylphosphate + H2O
-
 Escherichia coli p-nitrophenol + TMP
-
 ?
3.6.1.24 uridine 5'-p-nitrophenylphosphate + H2O
-
 Escherichia coli p-nitrophenol + UMP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.24 50
-
-
 Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.6.1.24 10 65 rapid loss of activity above 50°C Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.24
-
-
 no loss of activity within one day Escherichia coli
3.6.1.24 65
-
 loss of activity above 65°C, can be restored by cooling down to 0°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.24 7.5 8.5
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.24 5.5 10.5 very low activity at pH 5.5 and 10.5 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.6.1.24 7.5
-
 irreversible inactivation below pH 7.5 Escherichia coli