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Literature summary extracted from
- Deoxyuridine triphosphate nucleotidohydrolase induced by herpes simplex virus type 1. Purification and characterization of induced enzyme (1984), J. Biol. Chem., 259, 10080-10084.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.23 | 4-hydroxymercuribenzoate | 5 mM, complete inhibition | Homo sapiens |  |
| 3.6.1.23 | EDTA | reversed by Mg2+, Co2+ or Mn2+ | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.6.1.23 | 0.004 | - |
dUTP | enzyme from uninfected KB cells | Homo sapiens | |
| 3.6.1.23 | 0.0048 | - |
dUTP | enzyme from herpes simplex virus type 1 infected KB cells | Human alphaherpesvirus 1 | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.23 | Mg2+ | stimulates, enzyme also exhibits activity in absence of divalent cations | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.23 | 46000 | - |
enzyme from uninfected KB cells, gel filtration | Homo sapiens |
| 3.6.1.23 | 53000 | - |
enzyme from HSV-1 infected KB cells, gel filtration | Human alphaherpesvirus 1 |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.6.1.23 | Homo sapiens | - |
HSV-1-induced dUTPase and KB dUTPase | - |
| 3.6.1.23 | Human alphaherpesvirus 1 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.6.1.23 | - |
Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.6.1.23 | HeLa-S3 cell | - |
Homo sapiens | - |
| 3.6.1.23 | KB cell | KB cells induced or not induced with herpes simplex virus type 1 | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.23 | 1.46 | - |
enzyme from uninfected KB cells | Homo sapiens |
| 3.6.1.23 | 2.171 | - |
enzyme from HSV-1-infected cells | Human alphaherpesvirus 1 |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.6.1.23 | -20°C, 7 days, 2% loss of activity of HSV-1 induced dUTPase | Human alphaherpesvirus 1 |
| 3.6.1.23 | -20°C, 7 days, 70% loss of activity of uninduced dUTPase | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.1.23 | dUTP + H2O | - |
Homo sapiens | dUMP + diphosphate | - |
? | |
| 3.6.1.23 | dUTP + H2O | - |
Human alphaherpesvirus 1 | dUMP + diphosphate | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.23 | 45 | - |
t1/2: 35 min for enzyme from HSV-1 infected KB cells | Human alphaherpesvirus 1 |
| 3.6.1.23 | 45 | - |
t1/2: 22 min for enzyme from uninfected KB cells | Homo sapiens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.23 | 6.5 | 8.5 | enzyme from HSV-1 infected KB cells | Human alphaherpesvirus 1 |
| 3.6.1.23 | 7 | 8 | enzyme from uninfected KB cells | Homo sapiens |
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