Any feedback?
Literature summary extracted from
- Cloning and characterization of the NADH pyrophospatases from Caenorhabditis elegans and Saccharomyces cerevisiae, members of a nudix hydrolase subfamily (2000), Biochem. Biophys. Res. Commun., 273, 753-758.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.6.1.22 | expressed in Escherichia coli | Saccharomyces cerevisiae |
| 3.6.1.22 | expressed in Escherichia coli | Caenorhabditis elegans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.22 | Ca2+ | in presence of Mg2+ complete inhibition | Caenorhabditis elegans |  |
| 3.6.1.22 | Ca2+ | in presence of Mg2+ complete inhibition | Saccharomyces cerevisiae | |
| 3.6.1.22 | Zn2+ | in presence of Mg2+ complete inhibition | Caenorhabditis elegans | |
| 3.6.1.22 | Zn2+ | in presence of Mg2+ complete inhibition | Saccharomyces cerevisiae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.6.1.22 | 1.4 | - |
NADH | 50 mM Tris-HCl, pH 8.5, 5 mM MgCl2 | Caenorhabditis elegans | |
| 3.6.1.22 | 1.6 | - |
NADH | 50 mM Tris-HCl, pH 7.5, 5 mM MgCl2 | Saccharomyces cerevisiae | |
| 3.6.1.22 | 6.6 | - |
NAD+ | 50 mM Tris-HCl, pH 8.5, 5 mM MgCl2 | Caenorhabditis elegans | |
| 3.6.1.22 | 6.6 | - |
NAD+ | 50 mM Tris-HCl, pH 7.5, 5 mM MgCl2 | Saccharomyces cerevisiae | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.22 | Mg2+ | strong activation | Escherichia coli | |
| 3.6.1.22 | Mg2+ | strong activation | Saccharomyces cerevisiae | |
| 3.6.1.22 | Mg2+ | strong activation | Caenorhabditis elegans | |
| 3.6.1.22 | Mn2+ | strong activation | Escherichia coli | |
| 3.6.1.22 | Mn2+ | strong activation | Saccharomyces cerevisiae | |
| 3.6.1.22 | Mn2+ | strong activation | Caenorhabditis elegans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.22 | 39100 | - |
homodimer, 2 * 39100 | Caenorhabditis elegans |
| 3.6.1.22 | 43500 | - |
homodimer, 2 * 43500 | Saccharomyces cerevisiae |
| 3.6.1.22 | 78200 | - |
two subunits of 39100 Da found | Caenorhabditis elegans |
| 3.6.1.22 | 87000 | - |
two subunits of 43500 Da found | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.1.22 | NADH + H2O | Escherichia coli | possible function in regulation of NAD+/NADH-ratio | NMNH + AMP | - |
ir | |
| 3.6.1.22 | NADH + H2O | Saccharomyces cerevisiae | possible function in regulation of NAD+/NADH-ratio | NMNH + AMP | - |
ir | |
| 3.6.1.22 | NADH + H2O | Caenorhabditis elegans | possible function in regulation of NAD+/NADH-ratio | NMNH + AMP | - |
ir | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.6.1.22 | Caenorhabditis elegans | - |
- |
- |
| 3.6.1.22 | Escherichia coli | - |
- |
- |
| 3.6.1.22 | Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.6.1.22 | purification of gene product from Escherichia coli | Saccharomyces cerevisiae |
| 3.6.1.22 | purification of gene product from Escherichia coli | Caenorhabditis elegans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.1.22 | ADPribose + H2O | - |
Escherichia coli | ? | - |
? | |
| 3.6.1.22 | ADPribose + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| 3.6.1.22 | ADPribose + H2O | - |
Caenorhabditis elegans | ? | - |
? | |
| 3.6.1.22 | AppA + H2O | - |
Escherichia coli | ? | - |
? | |
| 3.6.1.22 | AppA + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| 3.6.1.22 | AppA + H2O | - |
Caenorhabditis elegans | ? | - |
? | |
| 3.6.1.22 | dATP + H2O | - |
Caenorhabditis elegans | ? | - |
? | |
| 3.6.1.22 | NAD+ + H2O | - |
Escherichia coli | NMN + AMP | - |
ir | |
| 3.6.1.22 | NAD+ + H2O | - |
Saccharomyces cerevisiae | NMN + AMP | - |
ir | |
| 3.6.1.22 | NAD+ + H2O | - |
Caenorhabditis elegans | NMN + AMP | - |
ir | |
| 3.6.1.22 | NADH + H2O | possible function in regulation of NAD+/NADH-ratio | Escherichia coli | NMNH + AMP | - |
ir | |
| 3.6.1.22 | NADH + H2O | possible function in regulation of NAD+/NADH-ratio | Saccharomyces cerevisiae | NMNH + AMP | - |
ir | |
| 3.6.1.22 | NADH + H2O | possible function in regulation of NAD+/NADH-ratio | Caenorhabditis elegans | NMNH + AMP | - |
ir | |
| 3.6.1.22 | NADH + H2O | - |
Escherichia coli | AMP + NMNH | - |
? | |
| 3.6.1.22 | NADH + H2O | - |
Saccharomyces cerevisiae | AMP + NMNH | - |
? | |
| 3.6.1.22 | NADH + H2O | - |
Caenorhabditis elegans | AMP + NMNH | - |
? | |
| 3.6.1.22 | P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | - |
Escherichia coli | ? | - |
? | |
| 3.6.1.22 | P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| 3.6.1.22 | P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | - |
Caenorhabditis elegans | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.6.1.22 | dimer | - |
Escherichia coli |
| 3.6.1.22 | dimer | homodimer, 2 * 39100 | Caenorhabditis elegans |
| 3.6.1.22 | dimer | homodimer, 2 * 43500 | Saccharomyces cerevisiae |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.6.1.22 | 0.000167 | - |
NAD+ | - |
Saccharomyces cerevisiae | |
| 3.6.1.22 | 0.000167 | - |
NAD+ | - |
Caenorhabditis elegans | |
| 3.6.1.22 | 0.00117 | - |
NADH | - |
Caenorhabditis elegans | |
| 3.6.1.22 | 0.0025 | - |
NADH | - |
Saccharomyces cerevisiae | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.22 | 7.5 | - |
- |
Saccharomyces cerevisiae |
| 3.6.1.22 | 8.5 | - |
- |
Escherichia coli |
| 3.6.1.22 | 8.5 | - |
- |
Caenorhabditis elegans |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
