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Literature summary extracted from

  • Nakajima, Y.; Fukunaga, N.; Sasaki, S.; Usami, S.
    Purification and properties of NADP pyrophosphatase from Proteus vulgaris (1973), Biochim. Biophys. Acta, 293, 242-255.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.22 2',5'-ADP 44% inhibition at concentration of 0.133 mM Proteus vulgaris
3.6.1.22 ADP 21% inhibition at concentration of 0.133 mM Proteus vulgaris
3.6.1.22 ATP 22% inhibition at concentration of 0.066 mM Proteus vulgaris
3.6.1.22 Ca2+ 20% inhibition at concentration of 0.33 mM Proteus vulgaris
3.6.1.22 Co2+ 40% inhibition of the purified enzyme at concentration of 0.33 mM, activity in crude extract not affected Proteus vulgaris
3.6.1.22 CoA 85% inhibition at concentration of 0.133 mM Proteus vulgaris
3.6.1.22 CTP 55% inhibition at concentration of 0.066 mM Proteus vulgaris
3.6.1.22 DNA strong inhibition by denaturated DNA, slight inhibition by native DNA Proteus vulgaris
3.6.1.22 EDTA 87% inhibition, reactivation by Mn2+ or Co2+ Proteus vulgaris
3.6.1.22 FAD 35% inhibition at concentration of 0.133 mM Proteus vulgaris
3.6.1.22 GTP 53% inhibition at concentration of 0.066 mM Proteus vulgaris
3.6.1.22 RNA strong inhibition Proteus vulgaris
3.6.1.22 UDP 32% inhibition at concentration of 0.133 mM Proteus vulgaris
3.6.1.22 UTP 36% inhibition at concentration of 0.066 mM Proteus vulgaris
3.6.1.22 Zn2+ 70% inhibition at concentration of 0.33 mM Proteus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.22 0.025
-
 NADP+ Tris-HCl, pH 7.0 Proteus vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.22 Co2+ reactivation of EDTA-treated enzyme Proteus vulgaris
3.6.1.22 Mg2+ slight activation at concentration of 1.0 mM Proteus vulgaris
3.6.1.22 Mn2+ slight activation at concentration of 1.0 mM, reactivation of EDTA-treated enzyme Proteus vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.22 NADP+ + H2O Proteus vulgaris possible function in regulation of NADP-level NMN + adenosine 3',5'-diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.22 Proteus vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.22
-
 Proteus vulgaris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.22 0.0008
-
 crude extract Proteus vulgaris
3.6.1.22 0.78
-
 purified enzyme with 1 mM MnCl2 Proteus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.22 ADP + H2O
-
 Proteus vulgaris AMP + phosphate
-
 ?
3.6.1.22 AMP + H2O
-
 Proteus vulgaris adenosine + phosphate
-
 ?
3.6.1.22 NAD+ + H2O
-
 Proteus vulgaris NMN + AMP
-
 ir
3.6.1.22 NADH + H2O
-
 Proteus vulgaris AMP + NMNH
-
 ?
3.6.1.22 NADP+ + H2O
-
 Proteus vulgaris NMN + adenosine 3',5'-diphosphate
-
 ?
3.6.1.22 NADP+ + H2O possible function in regulation of NADP-level Proteus vulgaris NMN + adenosine 3',5'-diphosphate
-
 ?
3.6.1.22 NADPH + H2O
-
 Proteus vulgaris ?
-
 ?
3.6.1.22 UDP + H2O
-
 Proteus vulgaris UMP + phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.22 7
-
-
 Proteus vulgaris