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Literature summary extracted from
- Uridine diphosphate sugar hydrolase. Purification of enzyme and protein inhibitor (1967), J. Biol. Chem., 242, 1944-1954.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.45 | ADP-D-glucose | competitive | Escherichia coli |  |
| 3.6.1.45 | CDP-D-glucose | competitive | Escherichia coli | |
| 3.6.1.45 | GDP-D-glucose | competitive | Escherichia coli | |
| 3.6.1.45 | Inhibitor from E. coli | - |
Escherichia coli |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.6.1.21 | 1.9 | - |
ADP-glucose | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| 3.6.1.21 | 40 | - |
GDP-Man | same Km for GDP-Glc and GDP-Man | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 3.6.1.21 | 40 | - |
GDP-Glc | same Km for GDP-Glc and GDP-Man | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 3.6.1.45 | additional information | - |
additional information | - |
Escherichia coli | |
| 3.6.1.45 | 0.002 | - |
UDP-D-glucose | with 5 mM CoSO4, pH 8.0, 37 °C | Escherichia coli | |
| 3.6.1.45 | 0.02 | - |
UDP-D-glucose | with 5 mM CoSO4, pH 8.0, 37 °C | Escherichia coli | |
| 3.6.1.45 | 0.11 | - |
GDP-D-glucose | - |
Escherichia coli | |
| 3.6.1.45 | 0.2 | - |
UDP-D-glucose | with 5 mM MgCl2, pH 8.0, 25 °C | Escherichia coli | |
| 3.6.1.45 | 0.33 | - |
CDP-D-glucose | - |
Escherichia coli | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.6.1.21 | soluble | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
| 3.6.1.21 | soluble | - |
Escherichia coli | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.21 | Mg2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| 3.6.1.21 | Mg2+ | - |
Escherichia coli | |
| 3.6.1.45 | Co2+ | absolute requirement for a divalent cation, at pH 8.0 in Tris-HCl buffer the requirement can be satisfied in order of decreasing affinity by Co2+, Mn2+, Mg2+. At pH 5.1 in acetate buffer only Co2+ and Mn2+ are effective | Escherichia coli | |
| 3.6.1.45 | Mg2+ | absolute requirement for a divalent cation, at pH 8.0 in Tris-HCl buffer the requirement can be satisfied in order of decreasing affinity by Co2+, Mn2+, Mg2+. At pH 5.1 in acetate buffer only Co2+ and Mn2+ are effective | Escherichia coli | |
| 3.6.1.45 | Mn2+ | absolute requirement for a divalent cation, at pH 8.0 in Tris-HCl buffer the requirement can be satisfied in order of decreasing affinity by Co2+, Mn2+, Mg2+. At pH 5.1 in acetate buffer only Co2+ and Mn2+ are effective | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.1.21 | ADP-sugar + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
AMP + sugar phosphate | - |
? | |
| 3.6.1.21 | ADP-sugar + H2O | Escherichia coli | - |
AMP + sugar phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.6.1.21 | Escherichia coli | - |
strain ATCC 12793 | - |
| 3.6.1.21 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
| 3.6.1.45 | Escherichia coli | - |
ATCC 12793 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.6.1.21 | partial purification together with UDP-sugar pyrophosphatase | Escherichia coli |
| 3.6.1.45 | - |
Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.45 | 12.58 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.1.21 | ADP-glucose + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | AMP + alpha-D-glucose 1-phosphate | - |
? | |
| 3.6.1.21 | ADP-sugar + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | AMP + sugar phosphate | - |
? | |
| 3.6.1.21 | ADP-sugar + H2O | - |
Escherichia coli | AMP + sugar phosphate | - |
? | |
| 3.6.1.21 | GDP-Glc + H2O | very poor substrate | Salmonella enterica subsp. enterica serovar Typhimurium | GMP + Glc-1-phosphate | - |
? | |
| 3.6.1.21 | GDP-Man + H2O | very poor substrate | Salmonella enterica subsp. enterica serovar Typhimurium | GMP + Man-1-phosphate | - |
? | |
| 3.6.1.45 | CDP-D-glucose | at 5% of the activity with UDP-glucose | Escherichia coli | CMP + glucose 1-phosphate | - |
? | |
| 3.6.1.45 | GDP-glucose | at 5% of the activity with UDP-glucose | Escherichia coli | GMP + glucose 1-phosphate | - |
? | |
| 3.6.1.45 | UDP-D-glucose | - |
Escherichia coli | UMP + glucose 1-phosphate | - |
? | |
| 3.6.1.45 | UDP-galactose | - |
Escherichia coli | UMP + galactose 1-phosphate | - |
? | |
| 3.6.1.45 | UDP-N-acetyl-D-galactosamine | - |
Escherichia coli | UMP + N-acetyl-D-galactosamine 1-phosphate | - |
? | |
| 3.6.1.45 | UDP-N-acetyl-D-glucosamine | - |
Escherichia coli | UMP + N-acetyl-D-glucosamine 1-phosphate | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.45 | additional information | - |
Co2+, Mn2+ or Mg2+ protect against heat inactivation | Escherichia coli |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.21 | 5 | - |
no activity even in presence of Mg2+ or Co2+ | Escherichia coli |
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