EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.53 | additional information | no inhibition by F- or adenosine 5'-tetraphosphate | Tetradesmus obliquus | |
3.6.1.17 | fluoride | - |
Chlorella vulgaris | |
3.6.1.17 | fluoride | - |
Scenedesmus sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.53 | 0.0053 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25°C | Tetradesmus obliquus | |
2.7.7.53 | 0.07 | - |
vanadate | P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25°C | Tetradesmus obliquus | |
2.7.7.53 | 0.13 | - |
arsenate | P1,P4-bis(5'-adenosyl) tetraphosphate-arsenolysis, pH 7.4, 25°C | Tetradesmus obliquus | |
2.7.7.53 | 0.16 | - |
phosphate | P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25°C | Tetradesmus obliquus | |
2.7.7.53 | 0.45 | - |
molybdate | P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25°C | Tetradesmus obliquus | |
2.7.7.53 | 0.52 | - |
chromate | P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25°C | Tetradesmus obliquus | |
2.7.7.53 | 1.75 | - |
tungstate | P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25°C | Tetradesmus obliquus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.53 | Ca2+ | less effective than Mg2+ | Tetradesmus obliquus | |
2.7.7.53 | Ca2+ | as good as Mn2+ | Tetradesmus obliquus | |
2.7.7.53 | Ca2+ | less effective than Co2+ | Tetradesmus obliquus | |
2.7.7.53 | Cd2+ | slight activation | Tetradesmus obliquus | |
2.7.7.53 | Co2+ | activation, less effective than Mn2+ or Ca2+ or Mg2+ | Tetradesmus obliquus | |
2.7.7.53 | divalent metal cations | requirement | Tetradesmus obliquus | |
2.7.7.53 | Mg2+ | - |
Tetradesmus obliquus | |
2.7.7.53 | Mn2+ | 10 mM | Tetradesmus obliquus | |
2.7.7.53 | Mn2+ | less effective than Mg2+ or Co2+ | Tetradesmus obliquus | |
2.7.7.53 | Zn2+ | slight activation | Tetradesmus obliquus | |
3.6.1.17 | Mg2+ | - |
Chlorella vulgaris | |
3.6.1.17 | Mg2+ | - |
Scenedesmus sp. | |
3.6.1.17 | Mn2+ | - |
Chlorella vulgaris | |
3.6.1.17 | Mn2+ | - |
Scenedesmus sp. | |
3.6.1.17 | Zn2+ | strongest activation | Chlorella vulgaris | |
3.6.1.17 | Zn2+ | strongest activation | Scenedesmus sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.7.53 | 46000 | - |
1 * 46000, SDS-PAGE | Tetradesmus obliquus |
2.7.7.53 | 46000 | 48000 | gel filtration | Tetradesmus obliquus |
3.6.1.17 | 20000 | 26000 | gel filtration, MW depends on subspecies | Scenedesmus sp. |
3.6.1.17 | 22000 | - |
gel filtration | Chlorella vulgaris |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.17 | P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | Chlorella vulgaris | - |
ATP + AMP | - |
? | |
3.6.1.17 | P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | Scenedesmus sp. | - |
ATP + AMP | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.53 | Chlorella vulgaris | - |
green alga | - |
2.7.7.53 | Scenedesmus basiliensis | - |
green alga | - |
2.7.7.53 | Scenedesmus quadricauda | - |
green alga | - |
2.7.7.53 | Tetradesmus obliquus | - |
green alga | - |
3.6.1.17 | Chlorella vulgaris | - |
- |
- |
3.6.1.17 | Scenedesmus sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.53 | P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis: 2600fold, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis: 8531fold | Tetradesmus obliquus |
3.6.1.17 | - |
Chlorella vulgaris |
3.6.1.17 | - |
Scenedesmus sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.7.53 | 0.00014 | - |
crude cell extract, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis | Scenedesmus quadricauda |
2.7.7.53 | 0.00065 | - |
crude cell extract, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis | Tetradesmus obliquus |
2.7.7.53 | 0.00096 | - |
crude cell extract, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis | Chlorella vulgaris |
2.7.7.53 | 0.0012 | - |
crude cell extract, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis | Scenedesmus basiliensis |
2.7.7.53 | 1.96 | - |
purified enzyme, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis | Tetradesmus obliquus |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.7.53 | -20°C, 30 mM HEPES/KOH buffer, pH 7.8, 50% glycerol, stable | Tetradesmus obliquus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.53 | ADP + arsenate | - |
Tetradesmus obliquus | AMP + phosphate + ? | - |
? | |
2.7.7.53 | ADP + ATP | - |
Tetradesmus obliquus | P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate | - |
r | |
2.7.7.53 | ADP + ATP | - |
Scenedesmus basiliensis | P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate | - |
r | |
2.7.7.53 | ADP + ATP | - |
Chlorella vulgaris | P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate | - |
r | |
2.7.7.53 | ADP + ATP | - |
Scenedesmus quadricauda | P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate | - |
r | |
2.7.7.53 | ADP + phosphate | ADP/phosphate exchange reaction | Tetradesmus obliquus | phosphate + ADP | - |
r | |
2.7.7.53 | additional information | no substrates are AMP, p-nitrophenylthymidine 5'-triphosphate, bis-p-nitrophenyl phosphate | Tetradesmus obliquus | ? | - |
? | |
2.7.7.53 | additional information | no activity with sulfate | Tetradesmus obliquus | ? | - |
? | |
2.7.7.53 | additional information | no activity with sulfate | Scenedesmus basiliensis | ? | - |
? | |
2.7.7.53 | additional information | no activity with sulfate | Chlorella vulgaris | ? | - |
? | |
2.7.7.53 | additional information | no activity with sulfate | Scenedesmus quadricauda | ? | - |
? | |
2.7.7.53 | additional information | no substrates are ADP, ATP, NAD+ | Tetradesmus obliquus | ? | - |
? | |
2.7.7.53 | P1,P3-bis(5'-adenosyl) triphosphate + phosphate | no activity with | Tetradesmus obliquus | ADP | - |
? | |
2.7.7.53 | P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate | - |
Tetradesmus obliquus | ? | - |
? | |
2.7.7.53 | P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate | - |
Scenedesmus basiliensis | ? | - |
? | |
2.7.7.53 | P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate | - |
Chlorella vulgaris | ? | - |
? | |
2.7.7.53 | P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate | - |
Scenedesmus quadricauda | ? | - |
? | |
2.7.7.53 | P1,P4-bis(5'-adenosyl) tetraphosphate + chromate | - |
Tetradesmus obliquus | ? | - |
r | |
2.7.7.53 | P1,P4-bis(5'-adenosyl) tetraphosphate + molybdate | - |
Tetradesmus obliquus | ? | - |
r | |
2.7.7.53 | P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate | - |
Tetradesmus obliquus | ADP + ATP | - |
r | |
2.7.7.53 | P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate | - |
Scenedesmus basiliensis | ADP + ATP | - |
r | |
2.7.7.53 | P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate | - |
Chlorella vulgaris | ADP + ATP | - |
r | |
2.7.7.53 | P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate | - |
Scenedesmus quadricauda | ADP + ATP | - |
r | |
2.7.7.53 | P1,P4-bis(5'-adenosyl) tetraphosphate + tungstate | - |
Tetradesmus obliquus | ? | - |
r | |
2.7.7.53 | P1,P4-bis(5'-adenosyl) tetraphosphate + vanadate | - |
Tetradesmus obliquus | ? | - |
r | |
3.6.1.17 | P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | - |
Chlorella vulgaris | ATP + AMP | - |
? | |
3.6.1.17 | P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | - |
Scenedesmus sp. | ATP + AMP | - |
? | |
3.6.1.17 | P1,P5-bis(5'-adenosyl) pentaphosphate + H2O | - |
Chlorella vulgaris | ADP + ATP | - |
? | |
3.6.1.17 | P1,P5-bis(5'-adenosyl) pentaphosphate + H2O | - |
Scenedesmus sp. | ADP + ATP | - |
? | |
3.6.1.17 | P1,P6-bis(5'-adenosyl) hexaphosphate + H2O | - |
Chlorella vulgaris | 2 ATP | - |
? | |
3.6.1.17 | P1,P6-bis(5'-adenosyl) hexaphosphate + H2O | - |
Scenedesmus sp. | 2 ATP | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.7.53 | monomer | 1 * 46000, SDS-PAGE | Tetradesmus obliquus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.53 | Ap4A phosphorylase | - |
Tetradesmus obliquus |
2.7.7.53 | Ap4A phosphorylase | - |
Scenedesmus basiliensis |
2.7.7.53 | Ap4A phosphorylase | - |
Chlorella vulgaris |
2.7.7.53 | Ap4A phosphorylase | - |
Scenedesmus quadricauda |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.53 | 25 | - |
assay at, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis | Tetradesmus obliquus |
2.7.7.53 | 25 | - |
assay at, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis | Scenedesmus basiliensis |
2.7.7.53 | 25 | - |
assay at, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis | Chlorella vulgaris |
2.7.7.53 | 25 | - |
assay at, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis | Scenedesmus quadricauda |
2.7.7.53 | 30 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis assay at | Tetradesmus obliquus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.53 | 5.3 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis assay at | Tetradesmus obliquus |
2.7.7.53 | 7.5 | 9.5 | - |
Tetradesmus obliquus |
2.7.7.53 | 8 | - |
assay at | Tetradesmus obliquus |
2.7.7.53 | 8 | - |
assay at | Scenedesmus basiliensis |
2.7.7.53 | 8 | - |
assay at | Chlorella vulgaris |
2.7.7.53 | 8 | - |
assay at | Scenedesmus quadricauda |
3.6.1.17 | 9 | - |
- |
Chlorella vulgaris |
3.6.1.17 | 9 | - |
- |
Scenedesmus sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.53 | 6.5 | 9.8 | about 20% of maximal activity at pH 6.5 and about 80% of maximal activity at pH 9.8 | Tetradesmus obliquus |
3.6.1.17 | 6.5 | 9.8 | 35% activity at pH 6.5 and 80% activity at pH 9.8 | Chlorella vulgaris |
3.6.1.17 | 6.5 | 9.8 | 35% activity at pH 6.5 and 80% activity at pH 9.8 | Scenedesmus sp. |