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Literature summary extracted from

  • McLennan, A.G.; Mayers, E.; Hankin, S.; Thorne, N.M.; Prescott, M.; Powls, R.
    The green alga Scenedesmus obliquus contains both diadenosine 5',5'''-P1,P4-tetraphosphate (asymmetrical) pyrophosphohyrolase and phosphorylase activities (1994), Biochem. J., 300, 183-189.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.53 additional information no inhibition by F- or adenosine 5'-tetraphosphate Tetradesmus obliquus
3.6.1.17 fluoride
-
 Chlorella vulgaris
3.6.1.17 fluoride
-
 Scenedesmus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.53 0.0053
-
 P1,P4-bis(5'-adenosyl) tetraphosphate P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25°C Tetradesmus obliquus
2.7.7.53 0.07
-
 vanadate P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25°C Tetradesmus obliquus
2.7.7.53 0.13
-
 arsenate P1,P4-bis(5'-adenosyl) tetraphosphate-arsenolysis, pH 7.4, 25°C Tetradesmus obliquus
2.7.7.53 0.16
-
 phosphate P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25°C Tetradesmus obliquus
2.7.7.53 0.45
-
 molybdate P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25°C Tetradesmus obliquus
2.7.7.53 0.52
-
 chromate P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25°C Tetradesmus obliquus
2.7.7.53 1.75
-
 tungstate P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25°C Tetradesmus obliquus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.53 Ca2+ less effective than Mg2+ Tetradesmus obliquus
2.7.7.53 Ca2+ as good as Mn2+ Tetradesmus obliquus
2.7.7.53 Ca2+ less effective than Co2+ Tetradesmus obliquus
2.7.7.53 Cd2+ slight activation Tetradesmus obliquus
2.7.7.53 Co2+ activation, less effective than Mn2+ or Ca2+ or Mg2+ Tetradesmus obliquus
2.7.7.53 divalent metal cations requirement Tetradesmus obliquus
2.7.7.53 Mg2+
-
 Tetradesmus obliquus
2.7.7.53 Mn2+ 10 mM Tetradesmus obliquus
2.7.7.53 Mn2+ less effective than Mg2+ or Co2+ Tetradesmus obliquus
2.7.7.53 Zn2+ slight activation Tetradesmus obliquus
3.6.1.17 Mg2+
-
 Chlorella vulgaris
3.6.1.17 Mg2+
-
 Scenedesmus sp.
3.6.1.17 Mn2+
-
 Chlorella vulgaris
3.6.1.17 Mn2+
-
 Scenedesmus sp.
3.6.1.17 Zn2+ strongest activation Chlorella vulgaris
3.6.1.17 Zn2+ strongest activation Scenedesmus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.53 46000
-
 1 * 46000, SDS-PAGE Tetradesmus obliquus
2.7.7.53 46000 48000 gel filtration Tetradesmus obliquus
3.6.1.17 20000 26000 gel filtration, MW depends on subspecies Scenedesmus sp.
3.6.1.17 22000
-
 gel filtration Chlorella vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.17 P1,P4-bis(5'-adenosyl) tetraphosphate + H2O Chlorella vulgaris
-
 ATP + AMP
-
 ?
3.6.1.17 P1,P4-bis(5'-adenosyl) tetraphosphate + H2O Scenedesmus sp.
-
 ATP + AMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.53 Chlorella vulgaris
-
 green alga
-
2.7.7.53 Scenedesmus basiliensis
-
 green alga
-
2.7.7.53 Scenedesmus quadricauda
-
 green alga
-
2.7.7.53 Tetradesmus obliquus
-
 green alga
-
3.6.1.17 Chlorella vulgaris
-
-
-
3.6.1.17 Scenedesmus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.53 P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis: 2600fold, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis: 8531fold Tetradesmus obliquus
3.6.1.17
-
 Chlorella vulgaris
3.6.1.17
-
 Scenedesmus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.53 0.00014
-
 crude cell extract, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis Scenedesmus quadricauda
2.7.7.53 0.00065
-
 crude cell extract, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis Tetradesmus obliquus
2.7.7.53 0.00096
-
 crude cell extract, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis Chlorella vulgaris
2.7.7.53 0.0012
-
 crude cell extract, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis Scenedesmus basiliensis
2.7.7.53 1.96
-
 purified enzyme, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis Tetradesmus obliquus

Storage Stability

EC Number Storage Stability Organism
2.7.7.53 -20°C, 30 mM HEPES/KOH buffer, pH 7.8, 50% glycerol, stable Tetradesmus obliquus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.53 ADP + arsenate
-
 Tetradesmus obliquus AMP + phosphate + ?
-
 ?
2.7.7.53 ADP + ATP
-
 Tetradesmus obliquus P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
 r
2.7.7.53 ADP + ATP
-
 Scenedesmus basiliensis P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
 r
2.7.7.53 ADP + ATP
-
 Chlorella vulgaris P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
 r
2.7.7.53 ADP + ATP
-
 Scenedesmus quadricauda P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
 r
2.7.7.53 ADP + phosphate ADP/phosphate exchange reaction Tetradesmus obliquus phosphate + ADP
-
 r
2.7.7.53 additional information no substrates are AMP, p-nitrophenylthymidine 5'-triphosphate, bis-p-nitrophenyl phosphate Tetradesmus obliquus ?
-
 ?
2.7.7.53 additional information no activity with sulfate Tetradesmus obliquus ?
-
 ?
2.7.7.53 additional information no activity with sulfate Scenedesmus basiliensis ?
-
 ?
2.7.7.53 additional information no activity with sulfate Chlorella vulgaris ?
-
 ?
2.7.7.53 additional information no activity with sulfate Scenedesmus quadricauda ?
-
 ?
2.7.7.53 additional information no substrates are ADP, ATP, NAD+ Tetradesmus obliquus ?
-
 ?
2.7.7.53 P1,P3-bis(5'-adenosyl) triphosphate + phosphate no activity with Tetradesmus obliquus ADP
-
 ?
2.7.7.53 P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate
-
 Tetradesmus obliquus ?
-
 ?
2.7.7.53 P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate
-
 Scenedesmus basiliensis ?
-
 ?
2.7.7.53 P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate
-
 Chlorella vulgaris ?
-
 ?
2.7.7.53 P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate
-
 Scenedesmus quadricauda ?
-
 ?
2.7.7.53 P1,P4-bis(5'-adenosyl) tetraphosphate + chromate
-
 Tetradesmus obliquus ?
-
 r
2.7.7.53 P1,P4-bis(5'-adenosyl) tetraphosphate + molybdate
-
 Tetradesmus obliquus ?
-
 r
2.7.7.53 P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
 Tetradesmus obliquus ADP + ATP
-
 r
2.7.7.53 P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
 Scenedesmus basiliensis ADP + ATP
-
 r
2.7.7.53 P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
 Chlorella vulgaris ADP + ATP
-
 r
2.7.7.53 P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
 Scenedesmus quadricauda ADP + ATP
-
 r
2.7.7.53 P1,P4-bis(5'-adenosyl) tetraphosphate + tungstate
-
 Tetradesmus obliquus ?
-
 r
2.7.7.53 P1,P4-bis(5'-adenosyl) tetraphosphate + vanadate
-
 Tetradesmus obliquus ?
-
 r
3.6.1.17 P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
 Chlorella vulgaris ATP + AMP
-
 ?
3.6.1.17 P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
 Scenedesmus sp. ATP + AMP
-
 ?
3.6.1.17 P1,P5-bis(5'-adenosyl) pentaphosphate + H2O
-
 Chlorella vulgaris ADP + ATP
-
 ?
3.6.1.17 P1,P5-bis(5'-adenosyl) pentaphosphate + H2O
-
 Scenedesmus sp. ADP + ATP
-
 ?
3.6.1.17 P1,P6-bis(5'-adenosyl) hexaphosphate + H2O
-
 Chlorella vulgaris 2 ATP
-
 ?
3.6.1.17 P1,P6-bis(5'-adenosyl) hexaphosphate + H2O
-
 Scenedesmus sp. 2 ATP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.53 monomer 1 * 46000, SDS-PAGE Tetradesmus obliquus

Synonyms

EC Number Synonyms Comment Organism
2.7.7.53 Ap4A phosphorylase
-
 Tetradesmus obliquus
2.7.7.53 Ap4A phosphorylase
-
 Scenedesmus basiliensis
2.7.7.53 Ap4A phosphorylase
-
 Chlorella vulgaris
2.7.7.53 Ap4A phosphorylase
-
 Scenedesmus quadricauda

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.53 25
-
 assay at, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis Tetradesmus obliquus
2.7.7.53 25
-
 assay at, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis Scenedesmus basiliensis
2.7.7.53 25
-
 assay at, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis Chlorella vulgaris
2.7.7.53 25
-
 assay at, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis Scenedesmus quadricauda
2.7.7.53 30
-
 P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis assay at Tetradesmus obliquus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.53 5.3
-
 P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis assay at Tetradesmus obliquus
2.7.7.53 7.5 9.5
-
 Tetradesmus obliquus
2.7.7.53 8
-
 assay at Tetradesmus obliquus
2.7.7.53 8
-
 assay at Scenedesmus basiliensis
2.7.7.53 8
-
 assay at Chlorella vulgaris
2.7.7.53 8
-
 assay at Scenedesmus quadricauda
3.6.1.17 9
-
-
 Chlorella vulgaris
3.6.1.17 9
-
-
 Scenedesmus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.53 6.5 9.8 about 20% of maximal activity at pH 6.5 and about 80% of maximal activity at pH 9.8 Tetradesmus obliquus
3.6.1.17 6.5 9.8 35% activity at pH 6.5 and 80% activity at pH 9.8 Chlorella vulgaris
3.6.1.17 6.5 9.8 35% activity at pH 6.5 and 80% activity at pH 9.8 Scenedesmus sp.