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Literature summary extracted from

  • Canales, J.; Pinto, R.M.; Costas, M.J.; Hernandez, M.T.; Miro, A.; Bernet, D.; Fernandez, A.; Cameselle, J.C.
    Rat liver nucleoside diphosphosugar or diphosphoalcohol pyrophosphatase different from nucleotide pyrophosphatase or phosphodiesterase I: substrate specificities of Mg2+- and/or Mn2+-depeneent hydrolases acting on ADP-ribose (1995), Biochim. Biophys. Acta, 1246, 167-177.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.13 0.0005 0.001 ADPribose ADPRibase I Rattus norvegicus
3.6.1.13 0.015 0.03 ADPribose ADPRibase-Mn Rattus norvegicus
3.6.1.13 0.05 0.1 ADPribose ADPRibase II Rattus norvegicus
3.6.1.53 0.015
-
 ADP-ribose pH 7.5, 37°C Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.13 Mg2+ ADPRibase-I is highly specific for ADPribose and IDPribose in presence of Mg2+, but active also on non-reducing ADP-hexoses and dinucleotides when Mg2+ is replaced with Mn2+. ADPRibase-Mn is a Mn2+-dependent diphosphatase active on ADPribose and IDPribose, dinucleotides and CDP alcohols. ADPribase-II is a rather unspecific diphosphatase that, with Mg2+, is active on AMP or IMP containing NDP sugars and dinucleotides, and with Mn2+ also active on non-adenine NDP-sugars and CDP-alcohols Rattus norvegicus
3.6.1.13 Mn2+ ADPRibase-I is highly specific for ADPribose and IDPribose in presence of Mg2+, but active also on non-reducing ADP-hexoses and dinucleotides when Mg2+ is replaced with Mn2+. ADPRibase-Mn is a Mn2+-dependent diphosphatase active on ADPribose and IDPribose, dinucleotides and CDP alcohols. ADPribase-II is a rather unspecific diphosphatase that, with Mg2+, is active on AMP or IMP containing NDP sugars and dinucleotides, and with Mn2+ also active on non-adenine NDP-sugars and CDP-alcohols Rattus norvegicus
3.6.1.53 Mn2+ required Rattus norvegicus
3.6.1.53 additional information Mg2+ cannot substitute for Mn2+ Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.13 32000
-
 ADPRibase-Mn, gel filtration Rattus norvegicus
3.6.1.13 40000
-
 ADPRibase I, gel filtration Rattus norvegicus
3.6.1.13 47000
-
 ADPRibase II, gel filtration Rattus norvegicus
3.6.1.53 32000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.13 Rattus norvegicus
-
-
-
3.6.1.53 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.13 ADPRibaseI, ADPribase II and ADPRibase-Mn Rattus norvegicus
3.6.1.53
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.13 liver
-
 Rattus norvegicus
-
3.6.1.53 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.13 0.439
-
 ADPRibase I Rattus norvegicus
3.6.1.13 12.81
-
 ADPRibase II Rattus norvegicus
3.6.1.13 18.2
-
 ADPRibase-Mn Rattus norvegicus
3.6.1.53 18.2
-
 pH 7.5, 37°C Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.13 ADP-glucose + H2O ADPRibase II, very low activity with ADPRibase I Rattus norvegicus AMP + alpha-D-glucose 1-phosphate
-
 ?
3.6.1.13 ADP-mannose + H2O ADPRibase II, low activity with ADPRibase I Rattus norvegicus AMP + D-mannose 1-phosphate
-
 ?
3.6.1.13 ADPribose + H2O
-
 Rattus norvegicus AMP + D-ribose 5-phosphate
-
 ?
3.6.1.13 FAD + H2O ADPRibase-Mn and ADPRibase II, very low activity with ADPRibase I in presence of Mn2+ Rattus norvegicus ?
-
 ?
3.6.1.13 IDP-ribose + H2O
-
 Rattus norvegicus IMP + ribose 5-phosphate
-
 ?
3.6.1.13 additional information three diphosphatases: 1.ADPRibase-I: an ADP-ribose diphosphatase highly specific for ADPribose and IDPribose in presence of Mg2+, but active also on non-reducing ADP-hexoses and dinucleotides, not on NAD+, when Mg2+ is replaced with Mn2+, 2. ADPRibase-Mn: a Mn2+-dependent diphosphatase active on ADPribose and IDPribose, dinucleotides and CDP alcohols, 3. ADPribase-II: a rather unspecific diphosphatase that, with Mg2+, is active on AMP or IMP containing NDP sugars and dinucleotides, not on NAD+, and with Mn2+ also active on non-adenine NDP-sugars and CDP-alcohols Rattus norvegicus ?
-
 ?
3.6.1.13 NAD+ + H2O ADPRibase-Mn in presence of Mn2+, no activity with ADPRibase I and ADPRibase II Rattus norvegicus ?
-
 ?
3.6.1.13 NADH + H2O ADPRibase I, ADPribase II and ADPRibase-Mn in presence of Mn2+ Rattus norvegicus ?
-
 ?
3.6.1.53 adenosine 5'-diphospho-5'-adenosine + H2O
-
 Rattus norvegicus AMP 45% of the activity with ADP-ribose ?
3.6.1.53 ADP + H2O 27% of the activity with ADP-ribose Rattus norvegicus AMP + phosphate
-
 ?
3.6.1.53 ADP-ribose + H2O
-
 Rattus norvegicus AMP + D-ribose 5-phosphate
-
 ?
3.6.1.53 CDP-choline + H2O 140% of the activity with ADP-ribose Rattus norvegicus CMP + phosphocholine
-
 ?
3.6.1.53 CDP-ethanolamine + H2O 89% of the activity with ADP-ribose Rattus norvegicus CMP + phosphoethanolamine
-
 ?
3.6.1.53 CDP-glycerol + H2O 89% of the activity with ADP-ribose Rattus norvegicus CMP + phosphoglycerol
-
 ?
3.6.1.53 FAD + H2O
-
 Rattus norvegicus ? 39% of the activity with ADP-ribose ?
3.6.1.53 IDP-ribose + H2O
-
 Rattus norvegicus IMP + D-ribose 5-phosphate 105% of the activity with ADP-ribose ?
3.6.1.53 NAD+ + H2O
-
 Rattus norvegicus ? 23% of the activity with ADP-ribose ?
3.6.1.53 NADH + H+ + H2O
-
 Rattus norvegicus ? 25% of the activity with ADP-ribose ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.13 7 9 ADPRibase I and ADPRibase II Rattus norvegicus
3.6.1.13 9
-
 ADPRibase-Mn Rattus norvegicus
3.6.1.53 9
-
-
 Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.6.1.53 Rattus norvegicus isoelectric focusing
-
 4.3