BRENDA - Enzyme Database

T4 phage deoxyribonucleotide-synthesizing enzyme complex. Further studies on enzyme composition and regulation

Allen, J.R.; Lasser, G.W.; Goldman, D.A.; Booth, J.W.; Mathews, C.K.; J. Biol. Chem. 258, 5746-5753 (1983)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.1.2.8
structural gene 42 encodes enzyme
Escherichia virus T4
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.2.8
27000
-
2 * 27000
Escherichia virus T4
2.1.2.8
60000
-
-
Escherichia virus T4
2.7.4.6
110000
-
-
Escherichia coli
2.7.4.13
22000
-
-
Escherichia virus T4
3.6.1.12
59000
-
dCTPase-dUTPase is an enzyme activity of the dNTP-synthesizing complex
Escherichia virus T4
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
Escherichia virus T4
dCTP depresses the synthesis of 5-hydroxymethyldeoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
Escherichia virus T4
?
2.1.2.8
additional information
Escherichia virus T4
enzyme is part of a complex efficiently channeling DNA precursors to the replication apparatus
?
-
-
-
2.7.4.11
ATP + dAMP
Escherichia coli
-
ADP + dADP
-
-
?
2.7.4.11
ATP + dAMP
Escherichia coli 201
-
ADP + dADP
-
-
?
2.7.4.13
ATP + deoxynucleoside phosphate
Escherichia virus T4
enzyme induced during viral infection
ADP + deoxynucleoside diphosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.2.8
Escherichia virus T4
-
Escherichia coli infected with bacteriophage T4
-
2.7.4.6
Escherichia coli
-
infected by bacteriophage T4, host-coded enzyme from infected Escherichia coli is part of bacteriophage T4 dNTP-synthesizing multi-enzyme complex
-
2.7.4.11
Escherichia coli
-
infected with bacteriophage T4amBL292, a maturation defective phage mutant, host-coded activity which is a component of T4 dNTP-synthesizing enzyme complex
-
2.7.4.11
Escherichia coli 201
-
infected with bacteriophage T4amBL292, a maturation defective phage mutant, host-coded activity which is a component of T4 dNTP-synthesizing enzyme complex
-
2.7.4.13
Escherichia virus T4
-
from infected Escherichia coli cells; maturation defective phage mutant T4amBL292, phage-coded activity which is a component of T4 dNTP-synthezising enzyme complex
-
2.7.4.13
no activity in Escherichia coli
-
-
-
3.6.1.12
Escherichia virus T4
-
dCTPase-dUTPase is an enzyme activity of the dNTP-synthesizing complex
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.4.6
partial
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
209854
Escherichia virus T4
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
209854
Escherichia virus T4
?
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
dCTP depresses the synthesis of 5-hydroxymethyldeoxycytidylate
209854
Escherichia virus T4
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
209854
Escherichia virus T4
?
2.1.2.8
additional information
enzyme is part of a complex efficiently channeling DNA precursors to the replication apparatus
209854
Escherichia virus T4
?
-
-
-
-
2.7.4.6
NTP + nucleoside diphosphate
-
209854
Escherichia coli
NDP + NTP
-
209854
Escherichia coli
-
2.7.4.6
NTP + nucleoside diphosphate
-
209854
Escherichia coli 201
NDP + NTP
-
209854
Escherichia coli 201
-
2.7.4.11
ATP + dAMP
-
209854
Escherichia coli
ADP + dADP
-
-
-
?
2.7.4.11
ATP + dAMP
-
209854
Escherichia coli 201
ADP + dADP
-
-
-
?
2.7.4.13
ATP + dAMP
-
209854
Escherichia virus T4
ADP + dADP
-
-
-
?
2.7.4.13
ATP + deoxynucleoside phosphate
enzyme induced during viral infection
209854
Escherichia virus T4
ADP + deoxynucleoside diphosphate
-
-
-
?
3.6.1.12
dCTP + H2O
-
209854
Escherichia virus T4
dCMP + diphosphate
-
209854
Escherichia virus T4
?
Subunits
EC Number
Subunits
Commentary
Organism
2.1.2.8
dimer
2 * 27000
Escherichia virus T4
3.6.1.12
?
dCTPase-dUTPase is an enzyme activity of the dNTP-synthesizing complex, SDS-PAGE
Escherichia virus T4
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.2.8
5,10-methylenetetrahydrofolate
-
Escherichia virus T4
2.1.2.8
tetrahydrofolate
-
Escherichia virus T4
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.2.8
structural gene 42 encodes enzyme
Escherichia virus T4
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.2.8
5,10-methylenetetrahydrofolate
-
Escherichia virus T4
2.1.2.8
tetrahydrofolate
-
Escherichia virus T4
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.2.8
27000
-
2 * 27000
Escherichia virus T4
2.1.2.8
60000
-
-
Escherichia virus T4
2.7.4.6
110000
-
-
Escherichia coli
2.7.4.13
22000
-
-
Escherichia virus T4
3.6.1.12
59000
-
dCTPase-dUTPase is an enzyme activity of the dNTP-synthesizing complex
Escherichia virus T4
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
Escherichia virus T4
dCTP depresses the synthesis of 5-hydroxymethyldeoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
Escherichia virus T4
?
2.1.2.8
additional information
Escherichia virus T4
enzyme is part of a complex efficiently channeling DNA precursors to the replication apparatus
?
-
-
-
2.7.4.11
ATP + dAMP
Escherichia coli
-
ADP + dADP
-
-
?
2.7.4.11
ATP + dAMP
Escherichia coli 201
-
ADP + dADP
-
-
?
2.7.4.13
ATP + deoxynucleoside phosphate
Escherichia virus T4
enzyme induced during viral infection
ADP + deoxynucleoside diphosphate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.6
partial
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
209854
Escherichia virus T4
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
209854
Escherichia virus T4
?
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
dCTP depresses the synthesis of 5-hydroxymethyldeoxycytidylate
209854
Escherichia virus T4
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
209854
Escherichia virus T4
?
2.1.2.8
additional information
enzyme is part of a complex efficiently channeling DNA precursors to the replication apparatus
209854
Escherichia virus T4
?
-
-
-
-
2.7.4.6
NTP + nucleoside diphosphate
-
209854
Escherichia coli
NDP + NTP
-
209854
Escherichia coli
-
2.7.4.6
NTP + nucleoside diphosphate
-
209854
Escherichia coli 201
NDP + NTP
-
209854
Escherichia coli 201
-
2.7.4.11
ATP + dAMP
-
209854
Escherichia coli
ADP + dADP
-
-
-
?
2.7.4.11
ATP + dAMP
-
209854
Escherichia coli 201
ADP + dADP
-
-
-
?
2.7.4.13
ATP + dAMP
-
209854
Escherichia virus T4
ADP + dADP
-
-
-
?
2.7.4.13
ATP + deoxynucleoside phosphate
enzyme induced during viral infection
209854
Escherichia virus T4
ADP + deoxynucleoside diphosphate
-
-
-
?
3.6.1.12
dCTP + H2O
-
209854
Escherichia virus T4
dCMP + diphosphate
-
209854
Escherichia virus T4
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.2.8
dimer
2 * 27000
Escherichia virus T4
3.6.1.12
?
dCTPase-dUTPase is an enzyme activity of the dNTP-synthesizing complex, SDS-PAGE
Escherichia virus T4