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Literature summary extracted from

  • Harper, D.B.
    Fungal degradation of aromatic nitriles. Enzymology of C-N cleavage by Fusarium solani (1977), Biochem. J., 167, 685-692.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.5.1 4-hydroxymercuribenzoic acid 50% inhibition at 0.05 mM Fusarium solani
3.5.5.1 Ag+ complete inhibition at 0.01 mM Fusarium solani
3.5.5.1 Hg2+ complete inhibition at 0.01 mM Fusarium solani
3.5.5.1 iodoacetamide slight inhibition at 0.05 mM Fusarium solani
3.5.5.1 N-ethylmaleimide slight inhibition at 0.05 mM Fusarium solani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.5.1 0.39
-
 Benzonitrile
-
 Fusarium solani

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.1 76000
-
 8 * 76000, SDS-PAGE Fusarium solani
3.5.5.1 620000
-
 gel filtration Fusarium solani

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.5.1 3-cyanopyridine + 2 H2O Fusarium solani
-
 nicotinic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O Fusarium solani
-
 benzoic acid + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Fusarium solani
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1 to homogeneity, chromatography techniques Fusarium solani

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.5.1 cell culture use of benzonitrile as a sole source of nitrogen and carbon Fusarium solani
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.5.1 1.66
-
-
 Fusarium solani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 1,4-benzodinitrile + H2O best substrate Fusarium solani benzoic acid + NH3
-
 ?
3.5.5.1 2-cyanopyridine + 2 H2O
-
 Fusarium solani 2-pyridine carboxylic acid + NH3
-
 ?
3.5.5.1 2-fluorobenzonitrile + H2O
-
 Fusarium solani 2-fluorobenzoic acid + NH3
-
 ?
3.5.5.1 2-toluonitrile + H2O
-
 Fusarium solani NH3 + 2-methylbenzoate
-
 ?
3.5.5.1 3-bromobenzonitrile + H2O
-
 Fusarium solani 3-bromobenzoic acid + NH3
-
 ?
3.5.5.1 3-chlorobenzonitrile + H2O
-
 Fusarium solani 3-chlorobenzoic acid + NH3
-
 ?
3.5.5.1 3-cyanopyridine + 2 H2O
-
 Fusarium solani nicotinic acid + NH3
-
 ?
3.5.5.1 3-fluorobenzonitrile + H2O
-
 Fusarium solani 3-fluorobenzoic acid + NH3
-
 ?
3.5.5.1 3-hydroxybenzonitrile + H2O
-
 Fusarium solani 3-hydroxybenzoic acid + NH3
-
 ?
3.5.5.1 3-toluonitrile + H2O
-
 Fusarium solani NH3 + 3-methylbenzoate
-
 ?
3.5.5.1 4-bromobenzonitrile + H2O
-
 Fusarium solani 4-bromobenzoic acid + NH3
-
 ?
3.5.5.1 4-chlorobenzonitrile + H2O
-
 Fusarium solani 4-chlorobenzoic acid + NH3
-
 ?
3.5.5.1 4-cyanopyridine + 2 H2O
-
 Fusarium solani 4-pyridinecarboxylic acid + NH3
-
 ?
3.5.5.1 4-fluorobenzonitrile + H2O
-
 Fusarium solani 4-fluorobenzoic acid + NH3
-
 ?
3.5.5.1 4-nitrobenzonitrile + H2O
-
 Fusarium solani 4-nitrobenzoic acid + NH3
-
 ?
3.5.5.1 4-toluonitrile + H2O
-
 Fusarium solani NH3 + 4-methylbenzoate
-
 ?
3.5.5.1 benzonitrile + 2 H2O
-
 Fusarium solani benzoic acid + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.5.1 octamer 8 * 76000, SDS-PAGE Fusarium solani

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.5.1 50
-
 inactivation above Fusarium solani

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.5.1 7.8 9.1 optimal activity within Fusarium solani

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.5.1 0.05
-
 4-hydroxymercuribenzoic acid
-
 Fusarium solani