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Literature summary extracted from

  • Kobayashi, M.; Komeda, H.; Yanaka, N.; Nagasawa, T.; Yamada, H.
    Nitrilase from Rhodococcus rhodochrous J1. Sequencing and overexpression of the gene and identification of an essential cysteine residue (1992), J. Biol. Chem., 267, 20746-20751.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.1 expression in Escherichia coli Rhodococcus rhodochrous

Protein Variants

EC Number Protein Variants Comment Organism
3.5.5.1 C165A no activity Rhodococcus rhodochrous
3.5.5.1 C165S no activity Rhodococcus rhodochrous

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.5.1 5,5'-dithiobis(2-nitrobenzoic acid) complete loss of activity due to modification of Cys165 Rhodococcus rhodochrous

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.5.1 benzonitrile + 2 H2O Rhodococcus rhodochrous
-
 benzoic acid + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Rhodococcus rhodochrous
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1 highly purified, recombinant enzyme Rhodococcus rhodochrous

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.5.1 8.04
-
-
 Rhodococcus rhodochrous

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 benzonitrile + 2 H2O
-
 Rhodococcus rhodochrous benzoic acid + NH3
-
 ?