Any feedback?
Literature summary extracted from
- Transformation of aliphatic and aromatic nitriles by a nitrilase from Pseudomonas sp. (1999), Can. J. Microbiol., 45, 811-815.
No PubMed abstract available
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.5.1 | 0.75 | - |
potassium cyanide | - |
Pseudomonas sp. |  |
| 3.5.5.1 | 5.88 | - |
acrylonitrile | - |
Pseudomonas sp. | |
| 3.5.5.1 | 10.28 | - |
adiponitrile | - |
Pseudomonas sp. | |
| 3.5.5.1 | 11.26 | - |
acetonitrile | - |
Pseudomonas sp. | |
| 3.5.5.1 | 12.27 | - |
Benzonitrile | - |
Pseudomonas sp. | |
| 3.5.5.7 | additional information | - |
additional information | kinetics | Pseudomonas sp. | |
| 3.5.5.7 | 0.75 | - |
potassium cyanide | pH 7.0, 37°C, partially purified enzyme | Pseudomonas sp. | |
| 3.5.5.7 | 5.88 | - |
acrylonitrile | pH 7.0, 37°C, partially purified enzyme | Pseudomonas sp. | |
| 3.5.5.7 | 10.28 | - |
adiponitrile | pH 7.0, 37°C, partially purified enzyme | Pseudomonas sp. | |
| 3.5.5.7 | 11.26 | - |
acetonitrile | pH 7.0, 37°C, partially purified enzyme | Pseudomonas sp. | |
| 3.5.5.7 | 12.27 | - |
Benzonitrile | pH 7.0, 37°C, partially purified enzyme | Pseudomonas sp. | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.7 | 41000 | - |
native PAGE | Pseudomonas sp. |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.5.1 | acrylonitrile + H2O | Pseudomonas sp. | best substrate, no formation of amide | acrylic acid + NH3 | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | Pseudomonas sp. | - |
benzoic acid + NH3 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.5.1 | Pseudomonas sp. | - |
- |
- |
| 3.5.5.7 | Pseudomonas sp. | - |
from soil | - |
| 3.5.5.7 | Pseudomonas sp. S1 | - |
from soil | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.5.1 | partial | Pseudomonas sp. |
| 3.5.5.7 | 3-step procedure, partially, 10.9fold | Pseudomonas sp. |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.5.5.7 | R-CN + 2 H2O = R-COOH + NH3 | preferentially hydrolyses aliphatic nitriles, some of which are apparently not substrates for other known nitrilases, EC 3.5.5.1. Substrates include crotonitrile, acrylnitrile and glutaronitrile | Pseudomonas sp. |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.5.1 | cell culture | - |
Pseudomonas sp. | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.1 | 3.2 | - |
- |
Pseudomonas sp. |
| 3.5.5.1 | 17530 | - |
- |
Pseudomonas sp. |
| 3.5.5.7 | 17.53 | - |
partially purified enzyme | Pseudomonas sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.5.1 | acetonitrile + H2O | no formation of amide | Pseudomonas sp. | acetic acid + NH3 | - |
? | |
| 3.5.5.1 | acrylonitrile + H2O | best substrate, no formation of amide | Pseudomonas sp. | acrylic acid + NH3 | - |
? | |
| 3.5.5.1 | adiponitrile + H2O | no formation of amide | Pseudomonas sp. | adipic acid + NH3 | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | - |
Pseudomonas sp. | benzoic acid + NH3 | - |
? | |
| 3.5.5.1 | potassium cyanide + H2O | - |
Pseudomonas sp. | potassium carbonate + NH3 | - |
? | |
| 3.5.5.7 | acetonitrile + H2O | - |
Pseudomonas sp. | acetate + NH3 | - |
? | |
| 3.5.5.7 | acetonitrile + H2O | - |
Pseudomonas sp. S1 | acetate + NH3 | - |
? | |
| 3.5.5.7 | acrylonitrile + H2O | most effective substrate | Pseudomonas sp. | acrylate + NH3 | - |
? | |
| 3.5.5.7 | adiponitrile + H2O | - |
Pseudomonas sp. | adipate + NH3 | - |
? | |
| 3.5.5.7 | benzonitrile + 2 H2O | - |
Pseudomonas sp. | benzoic acid + NH3 | - |
? | |
| 3.5.5.7 | benzonitrile + 2 H2O | - |
Pseudomonas sp. S1 | benzoic acid + NH3 | - |
? | |
| 3.5.5.7 | butyronitrile + H2O | - |
Pseudomonas sp. | butyrate + NH3 | - |
? | |
| 3.5.5.7 | butyronitrile + H2O | - |
Pseudomonas sp. S1 | butyrate + NH3 | - |
? | |
| 3.5.5.7 | glutaronitrile + H2O | - |
Pseudomonas sp. | glutarate + NH3 | - |
? | |
| 3.5.5.7 | glutaronitrile + H2O | - |
Pseudomonas sp. S1 | glutarate + NH3 | - |
? | |
| 3.5.5.7 | phenylacetonitrile + 2 H2O | - |
Pseudomonas sp. | phenylacetic acid + NH3 | - |
? | |
| 3.5.5.7 | phenylacetonitrile + 2 H2O | - |
Pseudomonas sp. S1 | phenylacetic acid + NH3 | - |
? | |
| 3.5.5.7 | potassium cyanide + H2O | - |
Pseudomonas sp. | ? | - |
? | |
| 3.5.5.7 | R-CN + H2O | enzyme uses both aliphatic and aromatic nitriles, no formation of amide intermediate | Pseudomonas sp. | R-COOH + NH3 | - |
? | |
| 3.5.5.7 | succinodinitrile + H2O | - |
Pseudomonas sp. | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.5.7 | monomer | 1 * 41000, SDS-PAGE | Pseudomonas sp. |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.5.7 | nitrilase | - |
Pseudomonas sp. |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.7 | 37 | - |
assay at | Pseudomonas sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.7 | 7 | - |
assay at | Pseudomonas sp. |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
