Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Suzuki, N.; Iwai, K.
    The occurrence and properties of methenyltetrahydrofolate cyclohydrolase in plants (1973), Plant Cell Physiol., 14, 319-327.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.5.4.9 unstable, stabilized by addition of 25% glycerol Pisum sativum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.9 aminopterin slightly retards enzyme activity Pisum sativum
3.5.4.9 methotrexate slightly retards enzyme activity Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.9 0.04
-
 5,10-methenyltetrahydrofolate
-
 Oryctolagus cuniculus
3.5.4.9 0.04
-
 5,10-methenyltetrahydrofolate
-
 Pisum sativum
3.5.4.9 0.075
-
 5,10-methenyltetrahydrofolate
-
 Oryctolagus cuniculus
3.5.4.9 0.2
-
 10-formyltetrahydrofolate
-
 Pisum sativum
3.5.4.9 0.25
-
 5,10-methenyltetrahydrofolate
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.4.9 mitochondrion
-
 Pisum sativum 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.9 10-formyltetrahydrofolate Bos taurus
-
 5,10-methenyltetrahydrofolate + H2O
-
 r
3.5.4.9 10-formyltetrahydrofolate Oryctolagus cuniculus
-
 5,10-methenyltetrahydrofolate + H2O
-
 r
3.5.4.9 10-formyltetrahydrofolate Pisum sativum
-
 5,10-methenyltetrahydrofolate + H2O
-
 r
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Asparagus officinalis
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Bos taurus
-
 10-formyltetrahydrofolate
-
 r
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Oryctolagus cuniculus
-
 10-formyltetrahydrofolate
-
 r
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Spinacia oleracea
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Pisum sativum
-
 10-formyltetrahydrofolate
-
 r
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Lactuca sativa
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Lentinula edodes
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Flammulina velutipes
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Brassica sp.
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Vigna mungo
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Trifolium sp.
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Petroselinum sp.
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Allium sp.
-
 10-formyltetrahydrofolate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.9 Allium sp.
-
 onion
-
3.5.4.9 Asparagus officinalis
-
 asparagus
-
3.5.4.9 Bos taurus
-
 beef
-
3.5.4.9 Brassica sp.
-
 cabbage
-
3.5.4.9 Flammulina velutipes
-
 enokitake fungus
-
3.5.4.9 Lactuca sativa
-
-
-
3.5.4.9 Lentinula edodes
-
 shiitake fungus
-
3.5.4.9 Oryctolagus cuniculus
-
 rabbit
-
3.5.4.9 Petroselinum sp.
-
 parsley
-
3.5.4.9 Pisum sativum
-
 pea, L. var. Alaska
-
3.5.4.9 Spinacia oleracea
-
 spinach
-
3.5.4.9 Trifolium sp.
-
 trefoil
-
3.5.4.9 Vigna mungo
-
 mappe bean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.9
-
 Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.4.9 bulb
-
 Allium sp.
-
3.5.4.9 leaf
-
 Spinacia oleracea
-
3.5.4.9 leaf
-
 Pisum sativum
-
3.5.4.9 leaf
-
 Lactuca sativa
-
3.5.4.9 leaf
-
 Brassica sp.
-
3.5.4.9 leaf
-
 Trifolium sp.
-
3.5.4.9 leaf
-
 Petroselinum sp.
-
3.5.4.9 liver
-
 Bos taurus
-
3.5.4.9 liver
-
 Oryctolagus cuniculus
-
3.5.4.9 petiole
-
 Spinacia oleracea
-
3.5.4.9 root
-
 Spinacia oleracea
-
3.5.4.9 root
-
 Pisum sativum
-
3.5.4.9 seedling
-
 Pisum sativum
-
3.5.4.9 sprout
-
 Pisum sativum
-
3.5.4.9 sprout
-
 Vigna mungo
-
3.5.4.9 sprout green Asparagus officinalis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.9 0.28
-
 0 day-old seedling Pisum sativum
3.5.4.9 0.37
-
 9 day-old seedling Pisum sativum
3.5.4.9 0.44
-
 3 day-old seedling Pisum sativum
3.5.4.9 0.5
-
-
 Trifolium sp.
3.5.4.9 0.5
-
 5 day-old seedling Pisum sativum
3.5.4.9 0.55
-
 7 day-old seedling Pisum sativum
3.5.4.9 0.72
-
 6 day-old sprout Pisum sativum
3.5.4.9 0.87
-
-
 Vigna mungo
3.5.4.9 1.04
-
-
 Lentinula edodes
3.5.4.9 1.04
-
-
 Allium sp.
3.5.4.9 1.3
-
-
 Lactuca sativa
3.5.4.9 1.35
-
-
 Flammulina velutipes
3.5.4.9 1.9
-
-
 Brassica sp.
3.5.4.9 2.1
-
-
 Petroselinum sp.
3.5.4.9 2.4
-
 source leaf Spinacia oleracea
3.5.4.9 2.7
-
 source root Spinacia oleracea
3.5.4.9 3.7
-
 source petiole Spinacia oleracea
3.5.4.9 4.5
-
-
 Asparagus officinalis
3.5.4.9 32.9
-
 purified enzyme Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.9 10-formyltetrahydrofolate
-
 Bos taurus 5,10-methenyltetrahydrofolate + H2O
-
 r
3.5.4.9 10-formyltetrahydrofolate
-
 Oryctolagus cuniculus 5,10-methenyltetrahydrofolate + H2O
-
 r
3.5.4.9 10-formyltetrahydrofolate
-
 Pisum sativum 5,10-methenyltetrahydrofolate + H2O
-
 r
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Asparagus officinalis 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Bos taurus 10-formyltetrahydrofolate
-
 r
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Oryctolagus cuniculus 10-formyltetrahydrofolate
-
 r
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Spinacia oleracea 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Pisum sativum 10-formyltetrahydrofolate
-
 r
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Lactuca sativa 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Lentinula edodes 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Flammulina velutipes 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Brassica sp. 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Vigna mungo 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Trifolium sp. 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Petroselinum sp. 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Allium sp. 10-formyltetrahydrofolate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.4.9 50 60 67% activity lost at 50°C for 5 min, 80% activity lost at 50°C for 10 min, 91% activity lost at 60°C for 5 min Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.9 7.7
-
-
 Pisum sativum