Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tan, L.U.L.; Drury, E.J.; MacKenzie, R.E.
    Methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase. A multifunctional protein from porcine liver (1977), J. Biol. Chem., 252, 1117-1122.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.5.1.5 stable for 1 week, stored in 20% glycerol, 0.1 M potassium phosphate, pH 7.3 Sus scrofa
3.5.4.9 purified enzyme stored in 20% glycerol, 0.1 M potassium phosphate pH 7.3 stable for about 1 week Sus scrofa
3.5.4.9 quite unstable, loses all activity after 96 h of storage Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.5 folic acid competitive against methylenetetrahydrofolate Sus scrofa
1.5.1.5 NAD+
-
 Sus scrofa
1.5.1.5 NADPH
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.5 0.018
-
 NADP+
-
 Sus scrofa
1.5.1.5 0.022
-
 5,10-methylenetetrahydrofolate
-
 Sus scrofa
3.5.4.9 0.13
-
 5,10-methenyltetrahydrofolate
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.5 100000
-
 1 * 100000, methylenetetrahydrofolate dehydrogenase, formyltetrahydrofolate synthetase and methenyltetrahydrofolate cyclohydrolase combined, SDS-PAGE Sus scrofa
1.5.1.5 150000
-
 methylenetetrahydrofolate dehydrogenase, formyltetrahydrofolate synthetase and methenyltetrahydrofolate cyclohydrolase combined, gel filtration Sus scrofa
3.5.4.9 100000
-
-
 Sus scrofa
3.5.4.9 150000
-
 gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+ Sus scrofa
-
 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Sus scrofa
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Bos taurus
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Clostridium cylindrosporum
-
 10-formyltetrahydrofolate
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.5.4.9 Glycerol
-
 Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.5 Sus scrofa
-
-
-
3.5.4.9 Bos taurus
-
 beef
-
3.5.4.9 Clostridium cylindrosporum
-
 monofunctional enzyme
-
3.5.4.9 Sus scrofa
-
 pig, trifunctional enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.5 trifunctional enzyme: methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrase, formyltetrahydrofolate synthetase Sus scrofa
3.5.4.9
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.5 liver
-
 Sus scrofa
-
3.5.4.9 liver
-
 Sus scrofa
-
3.5.4.9 liver
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+
-
 Sus scrofa 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Sus scrofa 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Bos taurus 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Clostridium cylindrosporum 10-formyltetrahydrofolate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.5 monomer 1 * 100000, methylenetetrahydrofolate dehydrogenase, formyltetrahydrofolate synthetase and methenyltetrahydrofolate cyclohydrolase combined, SDS-PAGE Sus scrofa
3.5.4.9 monomer 1 * 100000, SDS-PAGE, single polypeptide chain Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.5 NADP+
-
 Sus scrofa