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Literature summary extracted from

  • Ljungdahl, L.G.; Clark, J.E.
    Purification of 5,10-methenyltetrahydrofolate cyclohydrolase from Clostridium formicoaceticum (1986), Methods Enzymol., 122, 385-391.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.9 0.19
-
 5,10-methenyltetrahydrofolate
-
 Clostridium formicaceticum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.9 25500
-
 2 * 25500, SDS-PAGE Clostridium formicaceticum
3.5.4.9 41000
-
 sedimentation equilibrium centrifugation Clostridium formicaceticum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.9 10-formyltetrahydrofolate Clostridium formicaceticum
-
 5,10-methenyltetrahydrofolate + H2O
-
 r
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Escherichia coli
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Saccharomyces cerevisiae
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Moorella thermoautotrophica
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Moorella thermoacetica
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Clostridium formicaceticum
-
 10-formyltetrahydrofolate
-
 r
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Acetobacterium woodii
-
 10-formyltetrahydrofolate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.9 Acetobacterium woodii
-
 bifunctional enzyme
-
3.5.4.9 Clostridium formicaceticum
-
 ATCC 23439, monofunctional enzyme
-
3.5.4.9 Escherichia coli
-
-
-
3.5.4.9 Moorella thermoacetica
-
 bifunctional enzyme
-
3.5.4.9 Moorella thermoautotrophica
-
 bifunctional enzyme
-
3.5.4.9 Saccharomyces cerevisiae
-
 yeast, trifunctional enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.9
-
 Clostridium formicaceticum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.9 469
-
-
 Clostridium formicaceticum
3.5.4.9 470
-
 purified enzyme, pH 7.2, 35°C Clostridium formicaceticum

Storage Stability

EC Number Storage Stability Organism
3.5.4.9 5°C, 50 mM phosphate buffer, pH 7.0, stable for at least 1 week Clostridium formicaceticum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.9 10-formyltetrahydrofolate
-
 Clostridium formicaceticum 5,10-methenyltetrahydrofolate + H2O
-
 r
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Escherichia coli 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Saccharomyces cerevisiae 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Moorella thermoautotrophica 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Moorella thermoacetica 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Clostridium formicaceticum 10-formyltetrahydrofolate
-
 r
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Acetobacterium woodii 10-formyltetrahydrofolate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.9 dimer
-
 Escherichia coli
3.5.4.9 dimer
-
 Saccharomyces cerevisiae
3.5.4.9 dimer 2 * 25500, SDS-PAGE Clostridium formicaceticum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.4.9 55
-
 70% activity lost within 2 min, rapidly inactivated at temperatures above Clostridium formicaceticum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.9 6.6 7.6
-
 Clostridium formicaceticum
3.5.4.9 7
-
-
 Clostridium formicaceticum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.4.9 5.8 8
-
 Clostridium formicaceticum