EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.4.4 | basic histone proteins | - |
Xenopus laevis | |
3.5.4.4 | heparin | - |
Xenopus laevis | |
3.5.4.4 | K+ | - |
Xenopus laevis | |
3.5.4.4 | lysozyme | - |
Xenopus laevis | |
3.5.4.4 | Na+ | - |
Xenopus laevis | |
3.5.4.4 | NH4+ | - |
Xenopus laevis | |
3.5.4.4 | spermidine | - |
Xenopus laevis | |
3.5.4.4 | thiol reagents | - |
Xenopus laevis | |
3.5.4.37 | ammonium acetate | 0.4 M, complete inhibition | Xenopus laevis | |
3.5.4.37 | Basic proteins | histones and lysozyme, 0.5 mg/ml, complete inhibition | Xenopus laevis | |
3.5.4.37 | Double-stranded RNA | excess of substrate inhibits | Xenopus laevis | |
3.5.4.37 | heparin | 0.5 mg/ml, complete inhibition | Xenopus laevis | |
3.5.4.37 | iodoacetate | 10 mM, complete inhibition | Xenopus laevis | |
3.5.4.37 | KCl | 0.2 M, complete inhibition | Xenopus laevis | |
3.5.4.37 | additional information | no inhibition: DNA (salmon sperm, 0.3 mg/ml), RNA (torula yeast, 0.3 mg/ml), acidic proteins (bovine serum albumin and casein, 2.0 mg/ml), polyglutamic acid (2.0 mg/ml), glutamic acid (2 mM), glutamine (2 mM), glyceraldehyde 6-phosphate (2 mM), CaCl2 (5.0 mM), MgCl2 (5.0 mM), EDTA (10 mM), EGTA (10 mM), ATP (10 mM), GTP (10 mM), 5'-AMP (10 mM), adenosine (10 mM), hypoxanthine (10 mM) | Xenopus laevis | |
3.5.4.37 | N-ethylmaleimide | 10 mM, complete inhibition | Xenopus laevis | |
3.5.4.37 | NaCl | 0.2 M, complete inhibition | Xenopus laevis | |
3.5.4.37 | potassium phosphate | - |
Xenopus laevis | |
3.5.4.37 | Sodium phosphate | - |
Xenopus laevis | |
3.5.4.37 | spermidine | 0.5 mg/ml, complete inhibition | Xenopus laevis | |
3.5.4.37 | TLCK | 1 mM, complete inhibition | Xenopus laevis | |
3.5.4.37 | ZnCl2 | 1 mM, complete inhibition | Xenopus laevis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.4.4 | 90000 | - |
HPLC | Xenopus laevis |
3.5.4.4 | 120000 | - |
SDS-PAGE | Xenopus laevis |
3.5.4.37 | 12000 | - |
x * 12000, the purified monomeric enzyme form is active, but it is not known if the enzyme functions strictly as a monomer, it may dimerize in the presence of substrate or associate in crude mixture with other cellular constituents, SDS-PAGE | Xenopus laevis |
3.5.4.37 | 120000 | - |
gel filtration | Xenopus laevis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.4.37 | adenine in double-stranded RNA + H2O | Xenopus laevis | - |
hypoxanthine in double-stranded RNA + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.4.4 | Xenopus laevis | - |
- |
- |
3.5.4.37 | Xenopus laevis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.4.4 | - |
Xenopus laevis |
3.5.4.37 | - |
Xenopus laevis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.5.4.37 | egg | - |
Xenopus laevis | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.4.4 | 0.12 | - |
- |
Xenopus laevis |
3.5.4.37 | 0.002 | - |
25°C, pH 7.9 | Xenopus laevis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.4.4 | double-stranded RNA + H2O | - |
Xenopus laevis | ? | - |
? | |
3.5.4.37 | adenine in double-stranded RNA + H2O | - |
Xenopus laevis | hypoxanthine in double-stranded RNA + NH3 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.4.37 | ? | x * 12000, the purified monomeric enzyme form is active, but it is not known if the enzyme functions strictly as a monomer, it may dimerize in the presence of substrate or associate in crude mixture with other cellular constituents, SDS-PAGE | Xenopus laevis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.4.37 | double-stranded RNA adenosine deaminase | - |
Xenopus laevis |
3.5.4.37 | dsRAD | - |
Xenopus laevis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.4.4 | 6 | 8.5 | - |
Xenopus laevis |
3.5.4.37 | 6 | 8.5 | Tris or phosphate buffer | Xenopus laevis |