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Literature summary extracted from

  • Jun, H.K.; Kim, T.S.; Sakai, T.
    Purification and characterization of extracellular adenosine deaminase from a Streptomyces sp. (1991), J. Ferment. Bioeng., 71, 6-11.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.4 Ag+
-
 Streptomyces sp.
3.5.4.4 Hg2+
-
 Streptomyces sp.
3.5.4.4 o-phenanthroline
-
 Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.4 0.17
-
 AMP
-
 Streptomyces sp.
3.5.4.4 0.22
-
 2'-deoxyadenosine
-
 Streptomyces sp.
3.5.4.4 0.29
-
 2',3'-isopropylidene adenosine
-
 Streptomyces sp.
3.5.4.4 0.5
-
 cAMP
-
 Streptomyces sp.
3.5.4.4 0.67
-
 adenosine
-
 Streptomyces sp.
3.5.4.4 2
-
 Adenosine-3'-monophosphate
-
 Streptomyces sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.4.4 extracellular
-
 Streptomyces sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.4 Fe3+
-
 Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.4 45000
-
 SDS-PAGE Streptomyces sp.
3.5.4.4 90000
-
 gel filtration Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.4 Streptomyces sp.
-
 J-845S
-
3.5.4.4 Streptomyces sp. J-845S
-
 J-845S
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.4
-
 Streptomyces sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.4 211
-
-
 Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.4 2',3'-isopropylideneadenosine + H2O
-
 Streptomyces sp. 2',3'-isopropylideneinosine + NH3
-
 ?
3.5.4.4 2',3'-isopropylideneadenosine + H2O
-
 Streptomyces sp. J-845S 2',3'-isopropylideneinosine + NH3
-
 ?
3.5.4.4 2'-deoxyadenosine + H2O
-
 Streptomyces sp. 2'-deoxyinosine + NH3
-
 ?
3.5.4.4 2'-deoxyadenosine + H2O
-
 Streptomyces sp. J-845S 2'-deoxyinosine + NH3
-
 ?
3.5.4.4 5'-AMP + H2O
-
 Streptomyces sp. 5'-IMP + NH3
-
 ?
3.5.4.4 5'-AMP + H2O
-
 Streptomyces sp. J-845S 5'-IMP + NH3
-
 ?
3.5.4.4 adenosine + H2O
-
 Streptomyces sp. inosine + NH3
-
 ?
3.5.4.4 adenosine + H2O
-
 Streptomyces sp. J-845S inosine + NH3
-
 ?
3.5.4.4 adenosine-3'-monophosphate + H2O
-
 Streptomyces sp. IMP-3'-monophosphate + NH3
-
 ?
3.5.4.4 adenosine-3'-monophosphate + H2O
-
 Streptomyces sp. J-845S IMP-3'-monophosphate + NH3
-
 ?
3.5.4.4 cAMP + H2O
-
 Streptomyces sp. ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.4 55
-
-
 Streptomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.4.4 30
-
 30°C and below Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.4 5 6
-
 Streptomyces sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.4.4 3.5 5.5
-
 Streptomyces sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.4.4 3.5 5.5 in sodium acetate buffer Streptomyces sp.