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Literature summary extracted from

  • Kimm, S.W.; Park, J.B.; Lee, I.S.
    Purification and characterization of guanine aminohydrolase from human liver (1985), Korean J. Biochem., 17, 139-148.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.3 5-aminoimidazole-4-carboxamide 29% inhibition Homo sapiens
3.5.4.3 5-aminoimidazole-4-carboxamide
-
Oryctolagus cuniculus
3.5.4.3 5-aminoimidazole-4-carboxamide
-
Rattus norvegicus
3.5.4.3 adenine
-
Homo sapiens
3.5.4.3 adenosine
-
Homo sapiens
3.5.4.3 hypoxanthine slight inhibition of 8% Homo sapiens
3.5.4.3 Inosine slight inhibition of 5% Homo sapiens
3.5.4.3 p-hydroxymercuribenzoate noncompetitive inhibitor, 0.1 mM, 37°C, rapid inactivation Homo sapiens
3.5.4.3 p-hydroxymercuribenzoate
-
Oryctolagus cuniculus
3.5.4.3 p-hydroxymercuribenzoate
-
Rattus norvegicus
3.5.4.3 xanthine
-
Homo sapiens
3.5.4.3 Xanthosine
-
Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.3 0.0074
-
guanine at pH 8 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.4.3 cytosol
-
Homo sapiens 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.3 55000
-
-
Homo sapiens
3.5.4.3 55000
-
liver, single polypeptide chain, gel filtration Rattus norvegicus
3.5.4.3 55000
-
liver, single polypeptide chain, gel filtration Oryctolagus cuniculus
3.5.4.3 100000
-
-
Oryctolagus cuniculus
3.5.4.3 100000
-
gel filtration Homo sapiens
3.5.4.3 110000
-
-
Rattus norvegicus
3.5.4.3 110000
-
liver, gel filtration Homo sapiens
3.5.4.3 110000
-
liver, gel filtration Oryctolagus cuniculus
3.5.4.3 220000
-
gel filtration without mercaptoethanol Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.3 guanine + H2O Homo sapiens
-
xanthine + NH3
-
ir
3.5.4.3 guanine + H2O Rattus norvegicus
-
xanthine + NH3
-
ir
3.5.4.3 guanine + H2O Oryctolagus cuniculus
-
xanthine + NH3
-
ir

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.3 Homo sapiens
-
human
-
3.5.4.3 Oryctolagus cuniculus
-
rabbit
-
3.5.4.3 Rattus norvegicus
-
rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.3
-
Homo sapiens
3.5.4.3
-
Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.4.3 liver
-
Homo sapiens
-
3.5.4.3 liver
-
Rattus norvegicus
-
3.5.4.3 liver
-
Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.3 8.61
-
-
Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.3 8-azaguanine + H2O
-
Rattus norvegicus 8-azaxanthine + NH3
-
?
3.5.4.3 8-azaguanine + H2O
-
Oryctolagus cuniculus 8-azaxanthine + NH3
-
?
3.5.4.3 8-azaguanine + H2O high substrate specificity, only 60% of the reaction rate with the natural substrate guanine Homo sapiens 8-azaxanthine + NH3
-
?
3.5.4.3 adenine + H2O
-
Homo sapiens allopurinol + NH3
-
?
3.5.4.3 adenosine + H2O
-
Homo sapiens inosine + NH3
-
?
3.5.4.3 AMP + H2O
-
Homo sapiens IMP + NH3
-
?
3.5.4.3 GMP + H2O
-
Homo sapiens XMP + NH3
-
?
3.5.4.3 guanine + H2O
-
Homo sapiens xanthine + NH3
-
ir
3.5.4.3 guanine + H2O
-
Rattus norvegicus xanthine + NH3
-
ir
3.5.4.3 guanine + H2O
-
Oryctolagus cuniculus xanthine + NH3
-
ir
3.5.4.3 guanosine + H2O
-
Homo sapiens xanthosine + NH3
-
?

Subunits

EC Number Subunits Comment Organism
3.5.4.3 dimer
-
Homo sapiens
3.5.4.3 dimer
-
Oryctolagus cuniculus
3.5.4.3 dimer 2 * 55000, 2 or 3 isoenzymes, polymeric nature, enzyme exists as monomer, dimer or tetramer Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.4.3 60
-
50% activity lost during 30 min incubation, after 60 min 40% activity remains Homo sapiens
3.5.4.3 60
-
30 min incubation, liver enzyme completely inactivated Rattus norvegicus
3.5.4.3 60
-
30 min incubation, liver enzyme completely inactivated Oryctolagus cuniculus
3.5.4.3 65
-
activity completely lost after 30 min incubation Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.3 6.8
-
-
Oryctolagus cuniculus
3.5.4.3 7.5 9.5
-
Rattus norvegicus
3.5.4.3 7.5 9.5
-
Oryctolagus cuniculus
3.5.4.3 7.5 9.5 beyond pH 9.5 activity decreases abruptly Homo sapiens
3.5.4.3 8
-
-
Homo sapiens
3.5.4.3 8
-
-
Rattus norvegicus
3.5.4.3 8
-
-
Oryctolagus cuniculus
3.5.4.3 8.5
-
-
Rattus norvegicus
3.5.4.3 8.5
-
-
Oryctolagus cuniculus
3.5.4.3 8.6
-
-
Oryctolagus cuniculus
3.5.4.3 9
-
-
Homo sapiens