Any feedback?
Literature summary extracted from
- Cytosine deaminase from Escherichia coli-production, purification and some characteristics (1986), Agric. Biol. Chem., 50, 1721-1730.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.4.1 | D-alanine | 1 mM, relative activity 120% | Escherichia coli |  |
| 3.5.4.1 | D-threonine | 1 mM, relative activity 120% | Escherichia coli | |
| 3.5.4.1 | DL-alpha-amino-n-butyric acid | 1 mM, relative activity 140% | Escherichia coli | |
| 3.5.4.1 | DL-homoserine | 1 mM, relative activity 110% | Escherichia coli | |
| 3.5.4.1 | DL-isoleucine | 1 mM, relative activity 130% | Escherichia coli | |
| 3.5.4.1 | DL-methionine | 1mM, relative activity 140% | Escherichia coli | |
| 3.5.4.1 | EDTA | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| 3.5.4.1 | EDTA | 1 mM, relative activity 101% | Escherichia coli | |
| 3.5.4.1 | glycine | 1 mM, relative activity 130% | Escherichia coli | |
| 3.5.4.1 | L-amino acids | - |
Escherichia coli | |
| 3.5.4.1 | L-amino acids | - |
Serratia marcescens | |
| 3.5.4.1 | L-amino acids | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| 3.5.4.1 | L-aspartic acid | - |
Serratia marcescens | |
| 3.5.4.1 | L-aspartic acid | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| 3.5.4.1 | L-aspartic acid | 1 mM, relative activity 130% | Escherichia coli | |
| 3.5.4.1 | L-Glutamic acid | - |
Serratia marcescens | |
| 3.5.4.1 | L-Glutamic acid | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| 3.5.4.1 | L-Glutamic acid | 1 mM, relative activity 120% | Escherichia coli | |
| 3.5.4.1 | L-glutamine | - |
Serratia marcescens | |
| 3.5.4.1 | L-glutamine | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| 3.5.4.1 | L-glutamine | 1 mM, relative activity 130% | Escherichia coli | |
| 3.5.4.1 | L-histidine | - |
Serratia marcescens | |
| 3.5.4.1 | L-histidine | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| 3.5.4.1 | L-histidine | 1 mM, relative activity 120% | Escherichia coli | |
| 3.5.4.1 | L-hydroxyproline | - |
Serratia marcescens | |
| 3.5.4.1 | L-hydroxyproline | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| 3.5.4.1 | L-hydroxyproline | 1 mM, relative activity 120% | Escherichia coli | |
| 3.5.4.1 | L-isoleucine | 1 mM, relative activity 130% | Escherichia coli | |
| 3.5.4.1 | L-leucine | 1 mM, relative activity 120% | Escherichia coli | |
| 3.5.4.1 | L-ornithine | 1 mM, relative activity 120% | Escherichia coli | |
| 3.5.4.1 | L-phenylalanine | 1 mM, relative activity 140% | Escherichia coli | |
| 3.5.4.1 | L-serine | 1 mM, relative activity 140% | Escherichia coli | |
| 3.5.4.1 | L-threonine | 1 mM, relative activity 120% | Escherichia coli | |
| 3.5.4.1 | L-valine | 1 mM, relative activity 120% | Escherichia coli | |
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.5.4.1 | medicine | - |
Saccharomyces cerevisiae |
| 3.5.4.1 | medicine | new approach to chemotherapy of cancer, using substrate 5-fluorocytosine orally and enzyme capsules implanted locally, using conversion of product 5-fluorouracil, a potent antineoplastic substance, which is strongly toxic for mammalian cells as chemotherapeutic tool against tumors, enzyme/prodrug gene therapy, artificial gene composed for metastatic colorectal carcinoma | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.4.1 | Ag+ | - |
Escherichia coli | |
| 3.5.4.1 | Cu2+ | - |
Escherichia coli | |
| 3.5.4.1 | Cu2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| 3.5.4.1 | Cu2+ | - |
Serratia marcescens | |
| 3.5.4.1 | Hg(CH3COO)2 | - |
Escherichia coli | |
| 3.5.4.1 | Hg+ | - |
Escherichia coli | |
| 3.5.4.1 | Hg2+ | - |
Escherichia coli | |
| 3.5.4.1 | Hg2+ | - |
Serratia marcescens | |
| 3.5.4.1 | Mersalyl acid | - |
Escherichia coli | |
| 3.5.4.1 | p-chloromercuribenzoic acid | - |
Escherichia coli | |
| 3.5.4.1 | p-chloromercuriphenylsulfonic acid | - |
Escherichia coli | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.4.1 | CaCl2 | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| 3.5.4.1 | CaCl2 | 1 mM, relative activity 111% | Escherichia coli | |
| 3.5.4.1 | MnCl2 | 1 mM, relative activity 113% | Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.1 | 35000 | - |
2 * 35000 + 2 * 46000, heterodimer, SDS-PAGE | Escherichia coli |
| 3.5.4.1 | 46000 | - |
2 * 35000 + 2 * 46000, heterodimer, SDS-PAGE | Escherichia coli |
| 3.5.4.1 | 200000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.1 | cytosine + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
uracil + NH3 | - |
? | |
| 3.5.4.1 | cytosine + H2O | Escherichia coli | - |
uracil + NH3 | - |
? | |
| 3.5.4.1 | cytosine + H2O | Saccharomyces cerevisiae | - |
uracil + NH3 | - |
? | |
| 3.5.4.1 | cytosine + H2O | Serratia marcescens | - |
uracil + NH3 | - |
? | |
| 3.5.4.1 | cytosine + H2O | Pseudomonas chlororaphis subsp. aureofaciens | - |
uracil + NH3 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.4.1 | Escherichia coli | - |
K-12 IFO 3301 | - |
| 3.5.4.1 | Pseudomonas chlororaphis subsp. aureofaciens | - |
IFO 3521 | - |
| 3.5.4.1 | Saccharomyces cerevisiae | - |
baker's yeast | - |
| 3.5.4.1 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
| 3.5.4.1 | Serratia marcescens | - |
IFO 3054 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.4.1 | - |
Escherichia coli |
| 3.5.4.1 | - |
Saccharomyces cerevisiae |
| 3.5.4.1 | - |
Serratia marcescens |
| 3.5.4.1 | - |
Pseudomonas chlororaphis subsp. aureofaciens |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.1 | 9.8 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.1 | 5-fluorocytosine + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 5-fluorouracil + NH3 | - |
? | |
| 3.5.4.1 | 5-fluorocytosine + H2O | - |
Escherichia coli | 5-fluorouracil + NH3 | - |
? | |
| 3.5.4.1 | 5-fluorocytosine + H2O | - |
Saccharomyces cerevisiae | 5-fluorouracil + NH3 | - |
? | |
| 3.5.4.1 | cytosine + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | uracil + NH3 | - |
? | |
| 3.5.4.1 | cytosine + H2O | - |
Escherichia coli | uracil + NH3 | - |
? | |
| 3.5.4.1 | cytosine + H2O | - |
Saccharomyces cerevisiae | uracil + NH3 | - |
? | |
| 3.5.4.1 | cytosine + H2O | - |
Serratia marcescens | uracil + NH3 | - |
? | |
| 3.5.4.1 | cytosine + H2O | - |
Pseudomonas chlororaphis subsp. aureofaciens | uracil + NH3 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.4.1 | tetramer | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| 3.5.4.1 | tetramer | 2 * 35000 + 2 * 46000, heterodimer, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.1 | 50 | - |
- |
Escherichia coli |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.1 | 60 | - |
- |
Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.1 | 8.5 | - |
- |
Escherichia coli |
| 3.5.4.1 | 9 | - |
- |
Escherichia coli |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.1 | 9 | 10 | most stable at | Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
