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Literature summary extracted from

  • McGraw, W.T.; Potempa, J.; Farley, D.; Travis, J.
    Purification, characterization, and sequence analysis of a potential virulence factor from Porphyromonas gingivalis. Peptidylarginine deiminase (1999), Infect. Immun., 67, 3248-3256.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.5.3.15 FMN or FAD stabilize Porphyromonas gingivalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.15 antipain 8 mM, 41% inhibition Porphyromonas gingivalis
3.5.3.15 Cys 25 mM, complete inhibition Porphyromonas gingivalis
3.5.3.15 leupeptin 5 mM, complete inhibition Porphyromonas gingivalis
3.5.3.15 Nalpha-L-Arg methyl ester 12.5 mM, 48% inhibition Porphyromonas gingivalis
3.5.3.15 thiocitrulline 12.5 mM, complete inhibition Porphyromonas gingivalis
3.5.3.15 Thiourea 50 mM, complete inhibition Porphyromonas gingivalis
3.5.3.15 tosyl-L-leucinechloromethyl ketone 12.5 mM, 98% inhibition Porphyromonas gingivalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.15 0.0029
-
 benzoyl-L-Arg
-
 Porphyromonas gingivalis
3.5.3.15 0.028
-
 benzoyl-Gly-L-Arg
-
 Porphyromonas gingivalis
3.5.3.15 0.047
-
 bradykinin
-
 Porphyromonas gingivalis
3.5.3.15 0.141
-
 L-Arg
-
 Porphyromonas gingivalis
3.5.3.15 0.152
-
 benzoyl-L-Arg-NH2
-
 Porphyromonas gingivalis
3.5.3.15 0.874
-
 benzoyl-L-Arg ethyl ester
-
 Porphyromonas gingivalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.3.15 vesicular fraction 90% of the deiminase activity is cell- or membrane vesicle-associated Porphyromonas gingivalis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.15 46600
-
 1 * 46600, SDS-PAGE Porphyromonas gingivalis
3.5.3.15 47000
-
 gel filtration Porphyromonas gingivalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.15 protein L-Arg + H2O Porphyromonas gingivalis peptidylarginine deiminase, acting in concert with arginine-specific proteinases from Porphyromonas gingivalis, promotes the growth of the pathogen in the periodontal pocket, initially by enhancing its survivability and then by assisting the organism in its circumvention of host humoral defenses ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.15 Porphyromonas gingivalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.15
-
 Porphyromonas gingivalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.15 0.0334
-
-
 Porphyromonas gingivalis

Storage Stability

EC Number Storage Stability Organism
3.5.3.15 -20°C or -80°C, in presence of the stabilizing factors FMN or FAD, at pH 5.5, 7.5 or 9.0, no appreciable loss of activity Porphyromonas gingivalis
3.5.3.15 4°C, appreciable loss of activity after overnight storage Porphyromonas gingivalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.15 benzoyl-Arg ethyl ester + H2O
-
 Porphyromonas gingivalis benzoyl-citrulline ethyl ester + NH3
-
 ?
3.5.3.15 benzoyl-Gly-L-Arg + H2O
-
 Porphyromonas gingivalis benzoyl-Gly-L-citrulline + NH3
-
 ?
3.5.3.15 benzoyl-L-Arg + H2O
-
 Porphyromonas gingivalis benzoyl-L-citrulline + NH3
-
 ?
3.5.3.15 benzoyl-L-Arg-NH2 + H2O
-
 Porphyromonas gingivalis benzoyl-L-citrullineamide + NH3
-
 ?
3.5.3.15 bradykinin + H2O
-
 Porphyromonas gingivalis 9-citrulline bradykinin + NH3
-
 ?
3.5.3.15 L-Arg + H2O
-
 Porphyromonas gingivalis L-citrulline + NH3
-
 ?
3.5.3.15 additional information the enzyme undergoes automodification Porphyromonas gingivalis ?
-
 ?
3.5.3.15 protein L-Arg + H2O bradykinin Porphyromonas gingivalis protein L-citrulline + NH3
-
 ?
3.5.3.15 protein L-Arg + H2O peptidylarginine deiminase, acting in concert with arginine-specific proteinases from Porphyromonas gingivalis, promotes the growth of the pathogen in the periodontal pocket, initially by enhancing its survivability and then by assisting the organism in its circumvention of host humoral defenses Porphyromonas gingivalis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.15 monomer 1 * 46600, SDS-PAGE Porphyromonas gingivalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.15 55
-
-
 Porphyromonas gingivalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.15 9.3
-
-
 Porphyromonas gingivalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.3.15 7 11 pH 7.0: about 45% of maximal activity, pH 11.0: about 40% of maximal activity Porphyromonas gingivalis