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Literature summary extracted from

  • Srivenugopal, K.S.; Adiga, P.R.
    Enzymic conversion of agmatine to putrescine in Lathyrus sativus seedlings. Purification and properties of a multifunctional enzyme (putrescine synthase) (1981), J. Biol. Chem., 256, 9532-9541.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.3.3 prolonged dialysis and freeze-thawing causes loss of activity Lathyrus sativus
2.1.3.3 purified enzyme is highly unstable even in the presence of glycerol, dithiothreitol and Mg2+ Lathyrus sativus
2.1.3.6 highly unstable even in the presence of glycerol, dithiothreitol and Mg2+, activity is stabilized in dilute solutions, less than 0.05 mg protein/ml for about 3-4 h at 37°C by 0.25 mg/ml bovine serum albumine Lathyrus sativus
2.7.2.2 all the component activities associated with putrescine synthase are stabilized in dilute solutions, 0.05 mg of protein per ml, for about 3-4 h at 37°C by 0.250 mg/ml bovine serum albumin Lathyrus sativus
2.7.2.2 prolonged dialysis and freeze-thawing, loss of activity Lathyrus sativus
2.7.2.2 purified enzyme is highly unstable even in presence of glycerol, dithiothreitol and Mg2+ Lathyrus sativus
3.5.3.12 highly unstable even in presence of glycerol, dithiothreitol and Mg2+, freeze-thawing and dialysis lead to considerable loss of activity Lathyrus sativus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.3.3 cytoplasm
-
 Lathyrus sativus 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.3 55000
-
 1 * 55000, multifunctional enzyme, SDS-PAGE Lathyrus sativus
2.1.3.3 56000
-
 multifunctional enzyme with agmatine iminohydrolase, putrescine carbamoyltransferase, ornithine carbamoyltransferase and carbamate kinase activity, gel filtration Lathyrus sativus
2.1.3.6 55000
-
 1 * 55000, SDS-PAGE Lathyrus sativus
2.1.3.6 56000
-
 gel filtration Lathyrus sativus
3.5.3.12 55000
-
 gel filtration, PAGE Lathyrus sativus
3.5.3.12 56000
-
 gel filtration, Sephadex G-200 Lathyrus sativus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.2 ATP + NH3 + CO2 Lathyrus sativus multifunctional enzyme, involved in biosynthesis of putrescine ADP + carbamoyl phosphate
-
 r
3.5.3.12 agmatine + H2O Lathyrus sativus
-
 N-carbamoylputrescine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Lathyrus sativus
-
 putrescine synthase has inherent activities of agmatine iminohydrolase, putrescine carbamoyltransferase, ornithine carbamoyltransferase and carbamate kinase
-
2.1.3.6 Lathyrus sativus
-
-
-
2.7.2.2 Lathyrus sativus
-
 enzyme has inherent activities of agmatine iminohydrolase, putrescine transcarbamylase, ornithine transcarbamylase and carbamate kinase
-
3.5.3.12 Lathyrus sativus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.3 putrescine synthase has inherent activities of agmatine iminohydrolase, putrescine carbamoyltransferase, ornithine carbamoyltransferase and carbamate kinase, ammonium sulfate, putrescine-Sepharose affinity column Lathyrus sativus
2.1.3.6 MnCl2, ammonium sulfate, putrescine-CH-Sepharose affinity column Lathyrus sativus
2.7.2.2 enzyme has inherent activities of agmatine iminohydrolase, putrescine transcarbamylase, ornithine transcarbamylase and carbamate kinase Lathyrus sativus
3.5.3.12
-
 Lathyrus sativus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.3.6 carbamoyl phosphate + putrescine = phosphate + N-carbamoylputrescine multifunctional enzyme that catalyzes the reactions of EC 2.1.3.3 ornithine carbamoyltransferase, EC 2.7.2.2 carbamate kinase and EC 3.5.3.12 agmatine deiminase, thus acting as putrescine synthase, converting agmatine and ornithine into putrescine and citrulline, respectively Lathyrus sativus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.3.3 seedling
-
 Lathyrus sativus
-
2.1.3.6 seedling
-
 Lathyrus sativus
-
2.7.2.2 seedling
-
 Lathyrus sativus
-
3.5.3.12 seedling
-
 Lathyrus sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.3 0.47
-
 multifunctional enzyme Lathyrus sativus
3.5.3.12 1.1
-
-
 Lathyrus sativus

Storage Stability

EC Number Storage Stability Organism
2.1.3.3 4°C, 48 h, complete loss of activity Lathyrus sativus
2.1.3.6 4°C, 48 h, complete loss of activity Lathyrus sativus
2.7.2.2 4°C, 48 h, complete loss of activity Lathyrus sativus
3.5.3.12 4°C purified enzyme lost all component activities within 48 h Lathyrus sativus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine enzyme also catalyzes the reactions of EC 2.1.3.6, EC 2.7.2.2 and EC 3.5.3.12, thus acting as putrescine synthase converting agmatine and ornithine into putrescine and citrulline Lathyrus sativus phosphate + L-citrulline
-
 r
2.1.3.6 arsenate + N-carbamoylputrescine
-
 Lathyrus sativus ?
-
 ?
2.7.2.2 ADP + carbamoyl phosphate
-
 Lathyrus sativus ATP + NH3 + CO2
-
 r
2.7.2.2 ATP + NH3 + CO2
-
 Lathyrus sativus ADP + carbamoyl phosphate
-
 r
2.7.2.2 ATP + NH3 + CO2 multifunctional enzyme, involved in biosynthesis of putrescine Lathyrus sativus ADP + carbamoyl phosphate
-
 r
3.5.3.12 agmatine + H2O
-
 Lathyrus sativus N-carbamoylputrescine + NH3
-
 ?
3.5.3.12 agmatine + H2O multifunctional enzyme, acts also as putrescine transcarbamylase, putrescine synthase, ornithine transcarbamylase and carbamate kinase Lathyrus sativus N-carbamoylputrescine + NH3
-
 ?
3.5.3.12 agmatine + ornithine + H2O + phosphate
-
 Lathyrus sativus putrescine + citrulline + NH3 + phosphate
-
 ?
3.5.3.12 carbamoyl phosphate + ADP + H2O
-
 Lathyrus sativus ATP + CO2 + NH3
-
 r
3.5.3.12 carbamoyl phosphate + ornithine
-
 Lathyrus sativus citrulline + phosphate
-
 r
3.5.3.12 N-carbamoylputrescine + phosphate
-
 Lathyrus sativus putrescine + carbamoyl phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.3.3 monomer 1 * 55000, multifunctional enzyme, SDS-PAGE Lathyrus sativus
2.1.3.6 monomer 1 * 55000, SDS-PAGE Lathyrus sativus
3.5.3.12 monomer 1 * 55000, single polypeptide chain, SDS-PAGE Lathyrus sativus

Synonyms

EC Number Synonyms Comment Organism
2.7.2.2 putrescine synthase enzyme has inherent activities of agmatine iminohydrolase, putrescine transcarbamylase, ornithinetranscarbamylase and carbamate kinase Lathyrus sativus
3.5.3.12 putrescine synthase
-
 Lathyrus sativus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.3 8
-
-
 Lathyrus sativus
3.5.3.12 7.5
-
 putrescine transcarbamylase and carbamate kinase function Lathyrus sativus
3.5.3.12 8
-
 ornithine transcarbamylase function Lathyrus sativus
3.5.3.12 8.8
-
 agmatine iminohydrolase function Lathyrus sativus