EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.2.9 | 0.043 | - |
5-oxo-L-proline | - |
Pseudomonas putida | |
3.5.2.9 | 0.15 | - |
MgATP2- | - |
Pseudomonas putida |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.2.9 | K+ | - |
Rattus norvegicus | |
3.5.2.9 | K+ | maximal activity of component A as an ATPase requires 80-300 mM | Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.2.9 | 51000 | - |
2 protein compounds, component A exhibits 5-oxo-L-proline depedent ATPase activity, component A composed of 6 heterodimers, 6 * 51000 + 6 * 64000, SDS-PAGE, component B is a coupling protein without ATPase activity, 8 * 82000, octamer, SDS-PAGE | Pseudomonas putida |
3.5.2.9 | 64000 | - |
2 protein compounds, component A exhibits 5-oxo-L-proline depedent ATPase activity, component A composed of 6 heterodimers, 6 * 51000 + 6 * 64000, SDS-PAGE, component B is a coupling protein without ATPase activity, 8 * 82000, octamer, SDS-PAGE | Pseudomonas putida |
3.5.2.9 | 82000 | - |
2 protein compounds, component A exhibits 5-oxo-L-proline depedent ATPase activity, component A composed of 6 heterodimers, 6 * 51000 + 6 * 64000, SDS-PAGE, component B is a coupling protein without ATPase activity, 8 * 82000, octamer, SDS-PAGE | Pseudomonas putida |
3.5.2.9 | 115000 | - |
native molecular weight, component A, gel filtration | Pseudomonas putida |
3.5.2.9 | 650000 | - |
component B, gel filtration, native conditions | Pseudomonas putida |
3.5.2.9 | 750000 | - |
component A, gel filtration, native conditions | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.2.9 | 5-oxo-L-proline + H2O + ATP | Rattus norvegicus | - |
L-glutamate + ADP + phosphate | - |
? | |
3.5.2.9 | 5-oxo-L-proline + H2O + ATP | Pseudomonas putida | - |
L-glutamate + ADP + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.2.9 | Pseudomonas putida | - |
- |
- |
3.5.2.9 | Rattus norvegicus | - |
rat | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.2.9 | - |
Rattus norvegicus |
3.5.2.9 | separate purification of components A and B | Pseudomonas putida |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.5.2.9 | kidney | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.2.9 | 75 | - |
purified component B | Pseudomonas putida |
3.5.2.9 | 243 | - |
purified component A | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.2.9 | 5-oxo-L-proline + H2O + ATP | - |
Rattus norvegicus | L-glutamate + ADP + phosphate | - |
? | |
3.5.2.9 | 5-oxo-L-proline + H2O + ATP | - |
Pseudomonas putida | L-glutamate + ADP + phosphate | - |
? | |
3.5.2.9 | L-2-oxothiazolidine-4-carboxylate + H2O + ATP | - |
Rattus norvegicus | L-cysteine + ADP + phosphate | - |
? | |
3.5.2.9 | L-2-oxothiazolidine-4-carboxylate + H2O + ATP | - |
Pseudomonas putida | L-cysteine + ADP + phosphate | - |
? | |
3.5.2.9 | MgATP2- + H2O | - |
Pseudomonas putida | ? | - |
? | |
3.5.2.9 | additional information | no activity observed with 5-oxo-D-proline | Pseudomonas putida | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.2.9 | polymer | 2 protein compounds, component A exhibits 5-oxo-L-proline depedent ATPase activity, component A composed of 6 heterodimers, 6 * 51000 + 6 * 64000, SDS-PAGE, component B is a coupling protein without ATPase activity, 8 * 82000, octamer, SDS-PAGE | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.2.9 | 8.2 | - |
component A-catalyzed 5-oxoproline-dependent cleavage of ATP | Pseudomonas putida |
3.5.2.9 | 9.5 | - |
component B | Pseudomonas putida |