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Literature summary extracted from

  • May, O.; Siemann, M.; Pietzsch, M.; Kiess, M.; Mattes, R.; Syldatk, C.
    Substrate-dependent enantioselectivity of a novel hydantoinase from Arthrobacter aurescens DSM 3745: Purification and characterization as new member of cyclic amidases (1998), J. Biotechnol., 61, 1-13.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.2.2 synthesis
-
 Agrobacterium tumefaciens
3.5.2.2 synthesis production of enantiomeric pure nonproteinogenic amino acids, currently under investigation in bioprocess scale for industrial application Paenarthrobacter aurescens
3.5.2.2 synthesis commercial production of optically pure amino acids for synthesis of antibiotics, pharmaceuticals, artificial sweeteners and biologically active peptides, food ingredients, pyrethroids, pesticides, antimicrobial and antiviral agents and other agrochemicals Geobacillus stearothermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.2 D-hydantoinase gene cloned, sequenced and expressed in Escherichia coli Pseudomonas putida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.2.2
-
 Pseudomonas putida
3.5.2.2
-
 Paenarthrobacter aurescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.2 28
-
 DL-5-(2-methylthioethyl)-hydantoin
-
 Bos taurus
3.5.2.2 28
-
 DL-5-(2-methylthioethyl)-hydantoin
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.2 49670
-
 4 * 49670 Paenarthrobacter aurescens
3.5.2.2 49680
-
 MALDI-MS studies, single charged monomer Paenarthrobacter aurescens
3.5.2.2 53000
-
 SDS-PAGE Paenarthrobacter aurescens
3.5.2.2 200000
-
-
 Geobacillus stearothermophilus
3.5.2.2 200000
-
 native enzyme Paenarthrobacter aurescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.2 5,6-dihydrouracil + H2O Bos taurus
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Geobacillus stearothermophilus
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Pseudomonas putida
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Agrobacterium tumefaciens
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Paenarthrobacter aurescens
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Geobacillus stearothermophilus JC2310
-
 3-ureidopropionate
-
 ?
3.5.2.2 dihydrothymidine + H2O Paenarthrobacter aurescens
-
 ?
-
 ?
3.5.2.2 hydantoin + H2O Brevibacillus brevis
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Rattus norvegicus
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Bos taurus
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Geobacillus stearothermophilus
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas putida
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas stutzeri
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Arthrobacter sp.
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Agrobacterium tumefaciens
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Flavobacterium sp.
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Geobacillus stearothermophilus JC2310
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Arthrobacter sp. DSM 3747
-
 N-carbamoyl glycine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.2 Agrobacterium tumefaciens
-
-
-
3.5.2.2 Arthrobacter sp.
-
 DSM3747
-
3.5.2.2 Arthrobacter sp. DSM 3747
-
 DSM3747
-
3.5.2.2 Bos taurus
-
 calf
-
3.5.2.2 Bos taurus
-
 bovine
-
3.5.2.2 Brevibacillus brevis
-
-
-
3.5.2.2 Flavobacterium sp.
-
-
-
3.5.2.2 Geobacillus stearothermophilus
-
-
-
3.5.2.2 Geobacillus stearothermophilus JC2310
-
-
-
3.5.2.2 Paenarthrobacter aurescens
-
 DSM 3745
-
3.5.2.2 Pseudomonas putida
-
-
-
3.5.2.2 Pseudomonas stutzeri
-
-
-
3.5.2.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.2
-
 Pseudomonas putida
3.5.2.2
-
 Paenarthrobacter aurescens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.2.2 liver
-
 Rattus norvegicus
-
3.5.2.2 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.2 0.1
-
 10 mM dihydrouracil as substrate Paenarthrobacter aurescens
3.5.2.2 0.3
-
 DL-phenylhydantoin, 50 mM Paenarthrobacter aurescens
3.5.2.2 0.4
-
 10 mM dihydrothymine as substrate Paenarthrobacter aurescens
3.5.2.2 0.6
-
 DL-methylhydantoin, 57 mM Paenarthrobacter aurescens
3.5.2.2 1.5
-
 L-methylthioethylhydantoin, 2 mM Paenarthrobacter aurescens
3.5.2.2 5.4
-
 D-methylthioethylhydantoin, 2 mM Paenarthrobacter aurescens
3.5.2.2 12
-
 L-methylthioethylhydantoin, 20 mM Paenarthrobacter aurescens
3.5.2.2 13
-
 DL-5-indolylmethylhydantoin as substrate Paenarthrobacter aurescens
3.5.2.2 30
-
 DL-methylthioethylhydantoin, 40 mM Paenarthrobacter aurescens
3.5.2.2 37
-
 D-methylthioethylhydantoin, 20 mM Paenarthrobacter aurescens
3.5.2.2 70
-
 L-5-indolylmethylhydantoin as substrate Paenarthrobacter aurescens
3.5.2.2 72
-
-
 Paenarthrobacter aurescens
3.5.2.2 76.6
-
 DL-fluorobromylhydantoin as substrate Paenarthrobacter aurescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.2 5,6-dihydrouracil + H2O
-
 Bos taurus 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Geobacillus stearothermophilus 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Pseudomonas putida 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Agrobacterium tumefaciens 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Paenarthrobacter aurescens 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Geobacillus stearothermophilus JC2310 3-ureidopropionate
-
 ?
3.5.2.2 dihydrothymidine + H2O
-
 Paenarthrobacter aurescens ?
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Bos taurus N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Pseudomonas putida N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Paenarthrobacter aurescens N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-indolylmethylhydantoin + H2O
-
 Flavobacterium sp. L-tryptophan
-
 ?
3.5.2.2 DL-5-indolylmethylhydantoin + H2O strictly L-selective for the cleavage Paenarthrobacter aurescens L-tryptophan
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Rattus norvegicus N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Bos taurus N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Pseudomonas putida N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Flavobacterium sp. N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Paenarthrobacter aurescens N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Geobacillus stearothermophilus JC2310 N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-fluorobromylhydantoin + H2O
-
 Paenarthrobacter aurescens ?
-
 ?
3.5.2.2 DL-methylthioethylhydantoin + H2O D-selective for the hydrolysis Paenarthrobacter aurescens ?
-
 ?
3.5.2.2 hydantoin + H2O
-
 Bos taurus hydantoic acid
-
 ?
3.5.2.2 hydantoin + H2O
-
 Geobacillus stearothermophilus hydantoic acid
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas stutzeri hydantoic acid
-
 ?
3.5.2.2 hydantoin + H2O
-
 Geobacillus stearothermophilus JC2310 hydantoic acid
-
 ?
3.5.2.2 hydantoin + H2O
-
 Brevibacillus brevis N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Rattus norvegicus N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Bos taurus N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas putida N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas stutzeri N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Arthrobacter sp. N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Agrobacterium tumefaciens N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Flavobacterium sp. N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Geobacillus stearothermophilus JC2310 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Arthrobacter sp. DSM 3747 N-carbamoyl glycine
-
 ?
3.5.2.2 additional information
-
 Rattus norvegicus ?
-
 ?
3.5.2.2 additional information
-
 Bos taurus ?
-
 ?
3.5.2.2 additional information
-
 Pseudomonas putida ?
-
 ?
3.5.2.2 additional information hydantoin, urea, allantoin and dihydroorotate are no substrates Paenarthrobacter aurescens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.2 tetramer
-
 Rattus norvegicus
3.5.2.2 tetramer
-
 Bos taurus
3.5.2.2 tetramer
-
 Geobacillus stearothermophilus
3.5.2.2 tetramer
-
 Arthrobacter sp.
3.5.2.2 tetramer 4 * 49670 Paenarthrobacter aurescens