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Literature summary extracted from

  • Ogawa, J.; Soong, C.L.; Honda, M.; Shimizu, S.
    Imidase, a dihydropyrimidinase-like enzyme involved in the metabolism of cyclic imides (1997), Eur. J. Biochem., 243, 322-327.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.16 heavy metal ions
-
 Blastobacter sp. A17p-4
3.5.2.16 sulfhydryl reagents inhibition may be caused by modification of sulfhydryl residues involved in activity Blastobacter sp. A17p-4

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.16 0.34
-
 Maleimide
-
 Blastobacter sp. A17p-4
3.5.2.16 0.94
-
 succinimide
-
 Blastobacter sp. A17p-4
3.5.2.16 4.5
-
 glutarimide
-
 Blastobacter sp. A17p-4
3.5.2.16 5.7
-
 hydantoin
-
 Blastobacter sp. A17p-4
3.5.2.16 9.4
-
 beta-ureidopropionate
-
 Blastobacter sp. A17p-4
3.5.2.16 23
-
 thiohydantoin
-
 Blastobacter sp. A17p-4
3.5.2.16 31
-
 rhodanine
-
 Blastobacter sp. A17p-4
3.5.2.16 33
-
 2,4-Thiazolidinedione
-
 Blastobacter sp. A17p-4
3.5.2.16 52
-
 dihydrouracil
-
 Blastobacter sp. A17p-4
3.5.2.16 62
-
 parabanic acid
-
 Blastobacter sp. A17p-4

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.16 35000
-
 3 * 35000, SDS-PAGE Blastobacter sp. A17p-4
3.5.2.16 105000
-
 gel filtration Blastobacter sp. A17p-4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.16 dihydrothymine + H2O Blastobacter sp. A17p-4
-
 ?
-
 ?
3.5.2.16 dihydrouracil + H2O Blastobacter sp. A17p-4
-
 N-carbamoyl-beta-alanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.16 Blastobacter sp. A17p-4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.16
-
 Blastobacter sp. A17p-4

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.16 927
-
-
 Blastobacter sp. A17p-4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.16 2,4-thiazolidinedione + H2O
-
 Blastobacter sp. A17p-4 ?
-
 ?
3.5.2.16 dihydrothymine + H2O
-
 Blastobacter sp. A17p-4 ?
-
 ?
3.5.2.16 dihydrouracil + H2O
-
 Blastobacter sp. A17p-4 N-carbamoyl-beta-alanine
-
 ?
3.5.2.16 glutarimide + H2O
-
 Blastobacter sp. A17p-4 glutaric acid monoamide
-
 ?
3.5.2.16 hydantoin + H2O
-
 Blastobacter sp. A17p-4 hydantoic acid monoamide
-
 r
3.5.2.16 maleimide + H2O
-
 Blastobacter sp. A17p-4 maleic acid monoamide
-
 ?
3.5.2.16 parabanic acid + H2O
-
 Blastobacter sp. A17p-4 ?
-
 ?
3.5.2.16 rhodanine + H2O
-
 Blastobacter sp. A17p-4 ?
-
 ?
3.5.2.16 succinimide + H2O
-
 Blastobacter sp. A17p-4 succinamic acid monoamide
-
 r
3.5.2.16 thiohydantoin + H2O
-
 Blastobacter sp. A17p-4 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.16 trimer 3 * 35000, SDS-PAGE Blastobacter sp. A17p-4

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.16 60
-
-
 Blastobacter sp. A17p-4

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.2.16 20 60 more than 90% initial activity after incubation at 20-60°C for 30 min at pH 7.5, activity completely lost at 70°C Blastobacter sp. A17p-4

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.16 4.5 8.5 different pH profile for cyclic ureide and cyclic imide hydrolysis Blastobacter sp. A17p-4

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.2.16 5 9 more than 80% initial activity after incubation at 30°C for 30 min Blastobacter sp. A17p-4