BRENDA - Enzyme Database

Metabolism of cytosine, thymine, uracil, and barbituric acid by bacterial enzymes

Hayaishi, O.; Kornberg, A.; J. Biol. Chem. 197, 717-732 (1952)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.17.99.4
methylene blue
required as electron acceptor
Corynebacterium sp.
1.17.99.4
methylene blue
required as electron acceptor
Mycobacterium sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.99.4
0.035
-
thymine
-
Mycobacterium sp.
1.17.99.4
0.131
-
Uracil
-
Mycobacterium sp.
3.5.2.1
3.37
-
Barbiturate
-
Mycobacterium sp.
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.17.99.4
cytoplasm
-
Corynebacterium sp.
5737
-
1.17.99.4
cytoplasm
-
Mycobacterium sp.
5737
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.17.99.4
Corynebacterium sp.
-
-
-
1.17.99.4
Corynebacterium sp. 161
-
-
-
1.17.99.4
Mycobacterium sp.
-
-
-
3.5.2.1
Mycobacterium sp.
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.2.1
-
Mycobacterium sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.5.2.1
additional information
-
-
Mycobacterium sp.
Storage Stability
EC Number
Storage Stability
Organism
1.17.99.4
-10C, most stable at pH 9.0, no appreciable loss of activity for several months
Mycobacterium sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.99.4
thymine + acceptor
-
209264
Corynebacterium sp.
5-methylbarbiturate + reduced acceptor
-
209264
Corynebacterium sp.
?
1.17.99.4
thymine + acceptor
-
209264
Mycobacterium sp.
5-methylbarbiturate + reduced acceptor
-
209264
Mycobacterium sp.
?
1.17.99.4
thymine + acceptor
-
209264
Corynebacterium sp. 161
5-methylbarbiturate + reduced acceptor
-
209264
Corynebacterium sp. 161
?
1.17.99.4
uracil + acceptor
barbiturate, isobarbiturate, 5-methylbarbiturate, 6-methyluracil, dihydrothymine, dihydrouracil, 2-thiouracil, 2-thiothymine, 2-thio-5-methyluracil and cytosine are no substrates
209264
Corynebacterium sp.
barbiturate + reduced acceptor
-
209264
Corynebacterium sp.
?
1.17.99.4
uracil + acceptor
barbiturate, isobarbiturate, 5-methylbarbiturate, 6-methyluracil, dihydrothymine, dihydrouracil, 2-thiouracil, 2-thiothymine, 2-thio-5-methyluracil and cytosine are no substrates
209264
Mycobacterium sp.
barbiturate + reduced acceptor
-
209264
Mycobacterium sp.
?
1.17.99.4
uracil + acceptor
barbiturate, isobarbiturate, 5-methylbarbiturate, 6-methyluracil, dihydrothymine, dihydrouracil, 2-thiouracil, 2-thiothymine, 2-thio-5-methyluracil and cytosine are no substrates
209264
Corynebacterium sp. 161
barbiturate + reduced acceptor
-
209264
Corynebacterium sp. 161
?
3.5.2.1
barbiturate + H2O
-
209264
Mycobacterium sp.
malonate + urea
-
209264
Mycobacterium sp.
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.17.99.4
22
25
assay at
Corynebacterium sp.
1.17.99.4
22
25
assay at
Mycobacterium sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.99.4
8.5
-
-
Mycobacterium sp.
3.5.2.1
8
9
-
Mycobacterium sp.
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.17.99.4
8
10
about half-maximal activity at pH 8.0 and 10 with thymine as substrate
Mycobacterium sp.
1.17.99.4
8
9.7
about half-maximal activity at pH 8.0 and 9.7 with uracil as substrate
Mycobacterium sp.
3.5.2.1
7.5
9.5
pH 7.5: about 70% of maximal activity, pH 9.5: about 45% of maximal activity
Mycobacterium sp.
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.17.99.4
7
-
90% loss of activity
Mycobacterium sp.
1.17.99.4
9
-
most stable at -10C, no appreciable loss of activity for several months
Mycobacterium sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.17.99.4
additional information
no oxidation in the presence of O2 without methylene blue as electron acceptor
Corynebacterium sp.
1.17.99.4
additional information
no oxidation in the presence of O2 without methylene blue as electron acceptor
Mycobacterium sp.
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.17.99.4
methylene blue
required as electron acceptor
Corynebacterium sp.
1.17.99.4
methylene blue
required as electron acceptor
Mycobacterium sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.17.99.4
additional information
no oxidation in the presence of O2 without methylene blue as electron acceptor
Corynebacterium sp.
1.17.99.4
additional information
no oxidation in the presence of O2 without methylene blue as electron acceptor
Mycobacterium sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.99.4
0.035
-
thymine
-
Mycobacterium sp.
1.17.99.4
0.131
-
Uracil
-
Mycobacterium sp.
3.5.2.1
3.37
-
Barbiturate
-
Mycobacterium sp.
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.17.99.4
cytoplasm
-
Corynebacterium sp.
5737
-
1.17.99.4
cytoplasm
-
Mycobacterium sp.
5737
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.2.1
-
Mycobacterium sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.5.2.1
additional information
-
-
Mycobacterium sp.
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.17.99.4
-10C, most stable at pH 9.0, no appreciable loss of activity for several months
Mycobacterium sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.99.4
thymine + acceptor
-
209264
Corynebacterium sp.
5-methylbarbiturate + reduced acceptor
-
209264
Corynebacterium sp.
?
1.17.99.4
thymine + acceptor
-
209264
Mycobacterium sp.
5-methylbarbiturate + reduced acceptor
-
209264
Mycobacterium sp.
?
1.17.99.4
thymine + acceptor
-
209264
Corynebacterium sp. 161
5-methylbarbiturate + reduced acceptor
-
209264
Corynebacterium sp. 161
?
1.17.99.4
uracil + acceptor
barbiturate, isobarbiturate, 5-methylbarbiturate, 6-methyluracil, dihydrothymine, dihydrouracil, 2-thiouracil, 2-thiothymine, 2-thio-5-methyluracil and cytosine are no substrates
209264
Corynebacterium sp.
barbiturate + reduced acceptor
-
209264
Corynebacterium sp.
?
1.17.99.4
uracil + acceptor
barbiturate, isobarbiturate, 5-methylbarbiturate, 6-methyluracil, dihydrothymine, dihydrouracil, 2-thiouracil, 2-thiothymine, 2-thio-5-methyluracil and cytosine are no substrates
209264
Mycobacterium sp.
barbiturate + reduced acceptor
-
209264
Mycobacterium sp.
?
1.17.99.4
uracil + acceptor
barbiturate, isobarbiturate, 5-methylbarbiturate, 6-methyluracil, dihydrothymine, dihydrouracil, 2-thiouracil, 2-thiothymine, 2-thio-5-methyluracil and cytosine are no substrates
209264
Corynebacterium sp. 161
barbiturate + reduced acceptor
-
209264
Corynebacterium sp. 161
?
3.5.2.1
barbiturate + H2O
-
209264
Mycobacterium sp.
malonate + urea
-
209264
Mycobacterium sp.
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.17.99.4
22
25
assay at
Corynebacterium sp.
1.17.99.4
22
25
assay at
Mycobacterium sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.99.4
8.5
-
-
Mycobacterium sp.
3.5.2.1
8
9
-
Mycobacterium sp.
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.17.99.4
8
10
about half-maximal activity at pH 8.0 and 10 with thymine as substrate
Mycobacterium sp.
1.17.99.4
8
9.7
about half-maximal activity at pH 8.0 and 9.7 with uracil as substrate
Mycobacterium sp.
3.5.2.1
7.5
9.5
pH 7.5: about 70% of maximal activity, pH 9.5: about 45% of maximal activity
Mycobacterium sp.
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.17.99.4
7
-
90% loss of activity
Mycobacterium sp.
1.17.99.4
9
-
most stable at -10C, no appreciable loss of activity for several months
Mycobacterium sp.