BRENDA - Enzyme Database

Oxidative catabolism of uracil by Enterobacter aerogenes

Patel, B.N.; West, T.P.; FEMS Microbiol. Lett. 40, 33-36 (1987)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.17.99.4
methylene blue
required as electron acceptor
Klebsiella aerogenes
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.17.99.4
cytoplasm
-
Klebsiella aerogenes
5737
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.99.4
uracil + acceptor
Klebsiella aerogenes
reaction in two-step oxidative catabolism of uracil in prokaryotes
barbiturate + reduced acceptor
-
Klebsiella aerogenes
?
3.5.2.1
barbiturate + H2O
Klebsiella aerogenes
induced by uracil, enzyme is involved in the oxidative catabolism of uracil
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.17.99.4
Klebsiella aerogenes
-
-
-
3.5.2.1
Klebsiella aerogenes
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.17.99.4
0.0115
-
-
Klebsiella aerogenes
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.99.4
uracil + acceptor
barbiturate, isobarbiturate, 5-methylbarbiturate, 6-methyluracil, dihydrothymine, dihydrouracil, 2-thiouracil, 2-thiothymine, 2-thio-5-methyluracil and cytosine are no substrates
209263
Klebsiella aerogenes
barbiturate + reduced acceptor
-
209263
Klebsiella aerogenes
?
1.17.99.4
uracil + acceptor
reaction in two-step oxidative catabolism of uracil in prokaryotes
209263
Klebsiella aerogenes
barbiturate + reduced acceptor
-
209263
Klebsiella aerogenes
?
3.5.2.1
barbiturate + H2O
-
209263
Klebsiella aerogenes
malonate + urea
-
209263
Klebsiella aerogenes
?
3.5.2.1
barbiturate + H2O
induced by uracil, enzyme is involved in the oxidative catabolism of uracil
209263
Klebsiella aerogenes
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.17.99.4
30
-
assay at
Klebsiella aerogenes
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.17.99.4
2,6-dichlorophenolindophenol
required as electron acceptor, promotes barbiturate formation better than methylene blue
Klebsiella aerogenes
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.17.99.4
methylene blue
required as electron acceptor
Klebsiella aerogenes
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.17.99.4
2,6-dichlorophenolindophenol
required as electron acceptor, promotes barbiturate formation better than methylene blue
Klebsiella aerogenes
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.17.99.4
cytoplasm
-
Klebsiella aerogenes
5737
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.99.4
uracil + acceptor
Klebsiella aerogenes
reaction in two-step oxidative catabolism of uracil in prokaryotes
barbiturate + reduced acceptor
-
Klebsiella aerogenes
?
3.5.2.1
barbiturate + H2O
Klebsiella aerogenes
induced by uracil, enzyme is involved in the oxidative catabolism of uracil
?
-
-
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.17.99.4
0.0115
-
-
Klebsiella aerogenes
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.99.4
uracil + acceptor
barbiturate, isobarbiturate, 5-methylbarbiturate, 6-methyluracil, dihydrothymine, dihydrouracil, 2-thiouracil, 2-thiothymine, 2-thio-5-methyluracil and cytosine are no substrates
209263
Klebsiella aerogenes
barbiturate + reduced acceptor
-
209263
Klebsiella aerogenes
?
1.17.99.4
uracil + acceptor
reaction in two-step oxidative catabolism of uracil in prokaryotes
209263
Klebsiella aerogenes
barbiturate + reduced acceptor
-
209263
Klebsiella aerogenes
?
3.5.2.1
barbiturate + H2O
-
209263
Klebsiella aerogenes
malonate + urea
-
209263
Klebsiella aerogenes
?
3.5.2.1
barbiturate + H2O
induced by uracil, enzyme is involved in the oxidative catabolism of uracil
209263
Klebsiella aerogenes
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.17.99.4
30
-
assay at
Klebsiella aerogenes