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Literature summary extracted from

  • Shinohara, R.; Ishiguro, I.
    The purification and properties of formamidase from rat liver (1970), Biochim. Biophys. Acta, 198, 324-331.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.9 o-aminophenol activation at 0.2 mM Rattus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.9 Ag+ complete inhibition at 0.05 mM Rattus sp.
3.5.1.9 anthranilic acid 45% inhibition at 0.5 mM Rattus sp.
3.5.1.9 Cu2+ 31% inhibition at 1 mM Rattus sp.
3.5.1.9 Hg2+ complete inhibition at 0.05 mM Rattus sp.
3.5.1.9 Na2S2O4 27% inhibition at 0.04 mM Rattus sp.
3.5.1.9 NaAsO2 25% inhibition at 0.05 mM Rattus sp.
3.5.1.9 NaF 27% inhibition at 10 mM Rattus sp.
3.5.1.9 NaHSO3 24% inhibition at 0.04 mM Rattus sp.
3.5.1.9 o-aminobenzamidine 62% inhibition at 0.5 mM Rattus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.9 0.05
-
 N-formyl-L-kynurenine
-
 Rattus sp.
3.5.1.9 0.21
-
 N-formylanthranilic acid
-
 Rattus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.9 N-formyl-L-kynurenine + H2O Rattus sp.
-
 L-kynurenine + formate
-
 ?
3.5.1.9 N-formyl-o-aminoacetophenone + H2O Rattus sp. 60% of activity compared to N-formyl-L-kynurenine formate + o-aminoacetophenone
-
 ?
3.5.1.9 N-formylanthranilic acid + H2O Rattus sp. 13.5% activity compared to N-formyl-L-kynurenine formate + anthranilic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.9 Rattus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.9 to homogeneity, chromatography steps Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.9 liver
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.9 22.8
-
-
 Rattus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.9 formanilide + H2O poor activity Rattus sp. formate + aniline
-
 ?
3.5.1.9 N-formyl-L-kynurenine + H2O
-
 Rattus sp. L-kynurenine + formate
-
 ?
3.5.1.9 N-formyl-o-aminoacetophenone + H2O 60% of activity compared to N-formyl-L-kynurenine Rattus sp. formate + o-aminoacetophenone
-
 ?
3.5.1.9 N-formylanthranilic acid + H2O 13.5% activity compared to N-formyl-L-kynurenine Rattus sp. formate + anthranilic acid
-
 ?

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.9 5.5 9 optimum range Rattus sp.