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Literature summary extracted from

  • Mohapatra, B.R.; Bapuji, M
    Characterization of urethanase from Micrococcus species associated with the marine sponge (Spirastrella species) (1997), Lett. Appl. Microbiol., 25, 393-396.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.5.1.75 nutrition enzyme may be practically applicable in removal of urethane from alcoholic beverages, because very high ethanol resistance, high activity at acidic condition, pH 5.0 and very low Km value for urethane Micrococcus sp.
3.5.1.75 nutrition great advantage for industrial removal of urethane, potentially carcinogenic, mutagenic and teratogenic to human, from alcoholic beverages Micrococcus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.75 Co2+ 1 mM: 41% inhibition, 20 mM: complete inhibition Micrococcus sp.
3.5.1.75 Cu2+ 1 mM: 37% inhibition, 20 mM: 69% inhibition Micrococcus sp.
3.5.1.75 EDTA no inhibition Micrococcus sp.
3.5.1.75 Fe2+ 1 mM: 67% inhibition, 10 mM: complete inhibition Micrococcus sp.
3.5.1.75 iodoacetamide 0.1 mM: 48% inhibition Micrococcus sp.
3.5.1.75 iodoacetic acid 0.1 mM: 40% inhibition Micrococcus sp.
3.5.1.75 Mn2+ slight inhibition Micrococcus sp.
3.5.1.75 additional information no inhibition by EDTA Micrococcus sp.
3.5.1.75 Ni2+ 1 mM: 14% inhibition, 20 mM: 58% inhibition Micrococcus sp.
3.5.1.75 p-chloromercuribenzoate 0.1 mM: 52% inhibition Micrococcus sp.
3.5.1.75 phenylmethylsulfonyl fluoride 0.1 mM: 61% inhibition; PMSF Micrococcus sp.
3.5.1.75 Zn2+ 1 mM: 29% inhibition, 25 mM: complete inhibition Micrococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.75 additional information
-
 additional information kinetic properties Micrococcus sp.
3.5.1.75 0.078
-
 urethane
-
 Micrococcus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.75 additional information metal ions not required Micrococcus sp.
3.5.1.75 additional information no effect of Ca2+, Mg2+, Na+, major cations of sea water Micrococcus sp.

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.5.1.75 Ethanol 100% resistant to 10%, v/v, ethanol, above 10% ethanol the activity decreased depending upon ethanol concentration, resistant to 20%, v/v, ethanol: 82% activity remained Micrococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.75 Micrococcus sp.
-
 marine bacterium, associated with the marine sponge, Spirastrella species
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.75 partial Micrococcus sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.1.75 urethane + H2O = ethanol + CO2 + NH3 thermodynamic data Micrococcus sp.
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.75 cell culture 80% of urethanase activity is cell associated Micrococcus sp.
-
3.5.1.75 additional information 80% of urethanase activity is cell associated Micrococcus sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.75 ethylcarbamate + H2O urethane, potentially carcinogenic, mutagenic and teratogenic to human Micrococcus sp. CO2 + NH3 + ethanol noncarcinogenic compounds ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.75 45
-
-
 Micrococcus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.75 30 50 activity between Micrococcus sp.
3.5.1.75 50
-
 40% of maximum activity Micrococcus sp.
3.5.1.75 55
-
 22% of maximum activity Micrococcus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.75 45
-
 stable up to, 1 h, stability sharply declined above Micrococcus sp.
3.5.1.75 50
-
 half-life of enzyme at 50°C: 120 min Micrococcus sp.
3.5.1.75 60
-
 half-life of enzyme at 60°C: 30 min Micrococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.75 additional information
-
 significantly higher urethanase activity at acidic condition than at neutral condition Micrococcus sp.
3.5.1.75 5
-
-
 Micrococcus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.75 additional information
-
 urethanase activity sharply declines in alkaline region Micrococcus sp.
3.5.1.75 4 8.5 half of maximum activity at pH 4.0 and pH 8.5 Micrococcus sp.
3.5.1.75 6 7 pH 6.0: 98% of maximum activity, pH 7.0: 78% of maximum activity Micrococcus sp.