Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Martens, M.; Cottenie-Ruysschaert, M.; Hanselaer, R.; De Cooman, L.; Vande Casteele, K.; Van Sumere, C.F.
    N-Feruloylglycine amidohydrolase from barley seeds and isolated barley embryos (1988), Phytochemistry, 27, 2457-2463.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.71 (NH4)2SO4 0.4 M, enhances activity of unpurified enzyme by 55%, but no effect with purified enzyme Hordeum vulgare
3.5.1.71 K2SO4 0.4 M, enhances activity of unpurified enzyme by 36% Hordeum vulgare

General Stability

EC Number General Stability Organism
3.5.1.71 ammonium sulfate, 0.4 M, stabilizes during fractionation Hordeum vulgare
3.5.1.71 glycerol, 10%, stabilizes Hordeum vulgare

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.71 additional information
-
 Hordeum vulgare
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.71 N-feruloylglycine + H2O Hordeum vulgare best substrate ferulate + glycine
-
 ?
3.5.1.71 N-feruloylglycine + H2O Hordeum vulgare enzyme increases in activity during the first 14 h, maximum activity, of germination: enzyme plays a role during germination ferulate + glycine
-
 ?
3.5.1.71 N-feruloylglycine + H2O Hordeum vulgare may be responsible for the hydrolysis of N-feruloylglycyl-moieties of certain reserve proteins, because such N-feruloylglycine-containing proteins occur in barley seeds ferulate + glycine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.71 Hordeum vulgare
-
 barley
-
3.5.1.71 Hordeum vulgare
-
 cv Zephyr and cv Gitane
-
3.5.1.71 Lactuca sativa
-
 lettuce, very low activity
-
3.5.1.71 Pisum sativum
-
 pea, very low activity
-
3.5.1.71 Secale cereale
-
 rye, low activity
-
3.5.1.71 Spinacia oleracea
-
 spinach, very low activity
-
3.5.1.71 Trifolium sp.
-
 clover, very low activity
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.71 from germinated and excised embryos Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.71 aleurone layer
-
 Hordeum vulgare
-
3.5.1.71 embryo
-
 Pisum sativum
-
3.5.1.71 embryo
-
 Secale cereale
-
3.5.1.71 embryo highest activity in germinated barley embryos Hordeum vulgare
-
3.5.1.71 leaf
-
 Spinacia oleracea
-
3.5.1.71 leaf
-
 Lactuca sativa
-
3.5.1.71 leaf
-
 Trifolium sp.
-
3.5.1.71 additional information tissue distribution Hordeum vulgare
-
3.5.1.71 additional information tissue distribution Spinacia oleracea
-
3.5.1.71 additional information tissue distribution Pisum sativum
-
3.5.1.71 additional information tissue distribution Lactuca sativa
-
3.5.1.71 additional information tissue distribution Secale cereale
-
3.5.1.71 additional information tissue distribution Trifolium sp.
-
3.5.1.71 additional information no activity in endosperm, shoots and roots Hordeum vulgare
-
3.5.1.71 seed
-
 Hordeum vulgare
-
3.5.1.71 stem
-
 Trifolium sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.71 additional information
-
-
 Spinacia oleracea
3.5.1.71 additional information
-
-
 Pisum sativum
3.5.1.71 additional information
-
-
 Lactuca sativa
3.5.1.71 additional information
-
-
 Secale cereale
3.5.1.71 additional information
-
-
 Trifolium sp.
3.5.1.71 additional information
-
 highest activity in cv Gitane, almost twice the activity of cv Zephyr Hordeum vulgare
3.5.1.71 0.022
-
-
 Hordeum vulgare

Storage Stability

EC Number Storage Stability Organism
3.5.1.71 -28°C, stable for several months without serious loss of activity Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.71 additional information no activity with N-acetylglycine Hordeum vulgare ?
-
 ?
3.5.1.71 N-4-hydroxybenzoylglycine + H2O poorly hydrolyzed Hordeum vulgare 4-hydroxybenzoate + glycine
-
 ?
3.5.1.71 N-feruloylglycine + H2O
-
 Spinacia oleracea ferulate + glycine
-
 ?
3.5.1.71 N-feruloylglycine + H2O
-
 Pisum sativum ferulate + glycine
-
 ?
3.5.1.71 N-feruloylglycine + H2O
-
 Lactuca sativa ferulate + glycine
-
 ?
3.5.1.71 N-feruloylglycine + H2O
-
 Secale cereale ferulate + glycine
-
 ?
3.5.1.71 N-feruloylglycine + H2O
-
 Trifolium sp. ferulate + glycine
-
 ?
3.5.1.71 N-feruloylglycine + H2O best substrate Hordeum vulgare ferulate + glycine
-
 ?
3.5.1.71 N-feruloylglycine + H2O enzyme increases in activity during the first 14 h, maximum activity, of germination: enzyme plays a role during germination Hordeum vulgare ferulate + glycine
-
 ?
3.5.1.71 N-feruloylglycine + H2O may be responsible for the hydrolysis of N-feruloylglycyl-moieties of certain reserve proteins, because such N-feruloylglycine-containing proteins occur in barley seeds Hordeum vulgare ferulate + glycine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.71 N-FGAH
-
 Hordeum vulgare

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.71 30
-
 assay at Hordeum vulgare
3.5.1.71 32
-
-
 Hordeum vulgare

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.71 35
-
 stable up to, unstable above Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.71 8
-
-
 Hordeum vulgare

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.71 5
-
 unstable below Hordeum vulgare
3.5.1.71 7.5 10 very stable between Hordeum vulgare