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Literature summary extracted from

  • Miyake, M; Innami, T; Kakimoto, Y.
    A beta-citryl-L-glutamate-hydrolysing enzyme in rat testes (1983), Biochim. Biophys. Acta, 760, 206-214.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.68 Lubrol Px enzyme solubilized, activation Rattus norvegicus
3.5.1.68 additional information sulfhydryl reagent not required Rattus norvegicus
3.5.1.68 Triton X-100 enzyme solubilized, activation Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.68 adenine 1 mM: about 30% inhibition Rattus norvegicus
3.5.1.68 ADP 0.1 mM: about 30% inhibition, 1 mM: about 90% inhibition Rattus norvegicus
3.5.1.68 AMP 1 mM: about 30% inhibition Rattus norvegicus
3.5.1.68 ATP 0.1 mM: about 40% inhibition, 1 mM: about 95% inhibition; strong competitive inhibition Rattus norvegicus
3.5.1.68 citrate 0.1 mM: about 30% inhibition, 1 mM: 90% inhibition; inhibitory activity almost completely counteracted by 6 mM of Mn2+: inhibitory effect attributable to chelation of Mn2+ Rattus norvegicus
3.5.1.68 CuSO4 5 mM Rattus norvegicus
3.5.1.68 dithiothreitol 5 mM: very slight inhibition Rattus norvegicus
3.5.1.68 EDTA
-
 Rattus norvegicus
3.5.1.68 EGTA
-
 Rattus norvegicus
3.5.1.68 FeCl3 5 mM: strong inhibition Rattus norvegicus
3.5.1.68 FeSO4 5 mM Rattus norvegicus
3.5.1.68 GDP 1 mM: about 62% inhibition Rattus norvegicus
3.5.1.68 glutamate 1 mM: about 12% inhibition Rattus norvegicus
3.5.1.68 GMP 0.1 mM: about 16% inhibition, 1 mM: about 83% inhibition Rattus norvegicus
3.5.1.68 GTP 0.1 mM: about 66% inhibition, 1 mM: about 98% inhibition; strong competitive inhibition Rattus norvegicus
3.5.1.68 guanine 0.1 mM: about 11% inhibition, 1 mM: about 4% inhibition Rattus norvegicus
3.5.1.68 MgCl2 5 mM Rattus norvegicus
3.5.1.68 additional information no or very slight inhibition by adenosine, guanosine, cAMP, cGMP, S-adenosyl-L-homocysteine Rattus norvegicus
3.5.1.68 NiCl2 5 mM Rattus norvegicus
3.5.1.68 nucleotides strong inhibition Rattus norvegicus
3.5.1.68 phosphate 1 mM: slight inhibition; activity completely suppressed in phosphate buffer Rattus norvegicus
3.5.1.68 S-adenosyl-L-methionine 0.1 mM: about 6% inhibition, 1 mM: about 30% inhibition Rattus norvegicus
3.5.1.68 ZnCl2 5 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.68 0.0088
-
 beta-Citryl-L-glutamate
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.68 membrane localized in particulate fractions Rattus norvegicus 16020
-
3.5.1.68 microsome about 28% of total enzyme activity located in microsomal fractions Rattus norvegicus
-
-
3.5.1.68 mitochondrion about 70% of total enzyme activity located in mitochondrial fractions Rattus norvegicus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.68 Ca2+ required for full activity, can replace Mn2+ to some extent Rattus norvegicus
3.5.1.68 CaCl2 5 mM: high activation Rattus norvegicus
3.5.1.68 Mn2+ required for full activity Rattus norvegicus
3.5.1.68 Mn2+ maximal activity at 0.5-1 mM Rattus norvegicus
3.5.1.68 MnCl2 5 mM: most effective activation Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.68 Rattus norvegicus
-
 Wistar
-
3.5.1.68 Rattus norvegicus Wistar
-
 Wistar
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.68 partial Rattus norvegicus
3.5.1.68 solubilized by 0.5% Lubrol PX, most effective detergent Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.68 brain low activity Rattus norvegicus
-
3.5.1.68 heart moderate activity Rattus norvegicus
-
3.5.1.68 intestine moderate activity Rattus norvegicus
-
3.5.1.68 kidney moderate activity Rattus norvegicus
-
3.5.1.68 liver very low activity Rattus norvegicus
-
3.5.1.68 lung high activity, about 40% of the level in testis Rattus norvegicus
-
3.5.1.68 additional information tissue distribution, highest activity in testes and lung Rattus norvegicus
-
3.5.1.68 skeletal muscle low activity Rattus norvegicus
-
3.5.1.68 spleen very low activity Rattus norvegicus
-
3.5.1.68 stomach low activity Rattus norvegicus
-
3.5.1.68 testis highest activity Rattus norvegicus
-
3.5.1.68 thymus low activity Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.68 beta-citryl-L-glutamate + H2O at about 30% the rate of formylglutamate hydrolysis Rattus norvegicus citrate + L-glutamate
-
 ir
3.5.1.68 beta-citryl-L-glutamate + H2O deacylation of beta-citryl-L-glutamate Rattus norvegicus citrate + L-glutamate
-
 ir
3.5.1.68 beta-citryl-L-glutamate + H2O at about 30% the rate of formylglutamate hydrolysis Rattus norvegicus Wistar citrate + L-glutamate
-
 ir
3.5.1.68 beta-citryl-L-glutamine + H2O at about 13% the rate of formylglutamate hydrolysis Rattus norvegicus citrate + L-glutamine
-
 ir
3.5.1.68 additional information substrate specificity Rattus norvegicus ?
-
 ?
3.5.1.68 additional information little or no activity with N-formyl-L-aspartate, N-formyl-L-glutamine, N-acetyl-L-glutamate, alpha-citryl-L-glutamate, alpha- and beta-citryl-alpha-L-glutamylglutamate Rattus norvegicus ?
-
 ?
3.5.1.68 additional information little or no activity with N-acetyl-L-glutamate Rattus norvegicus ?
-
 ?
3.5.1.68 additional information substrate specificity Rattus norvegicus Wistar ?
-
 ?
3.5.1.68 additional information little or no activity with N-formyl-L-aspartate, N-formyl-L-glutamine, N-acetyl-L-glutamate, alpha-citryl-L-glutamate, alpha- and beta-citryl-alpha-L-glutamylglutamate Rattus norvegicus Wistar ?
-
 ?
3.5.1.68 additional information little or no activity with N-acetyl-L-glutamate Rattus norvegicus Wistar ?
-
 ?
3.5.1.68 N-acetyl-alpha-L-aspartylglutamate + H2O poor substrate Rattus norvegicus acetate + alpha-L-aspartylglutamate
-
 ?
3.5.1.68 N-acetyl-L-aspartate + H2O poor substrate Rattus norvegicus acetate + L-aspartate
-
 ?
3.5.1.68 N-acetyl-L-methionine + H2O poor substrate Rattus norvegicus acetate + L-methionine
-
 ?
3.5.1.68 N-formyl-L-glutamate + H2O best substrate Rattus norvegicus formate + L-glutamate
-
 ?
3.5.1.68 N-formyl-L-glutamate + H2O best substrate Rattus norvegicus Wistar formate + L-glutamate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.68 37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.68 50
-
 2 min: 5% loss of activity, 5 min: 10% loss of activity Rattus norvegicus
3.5.1.68 100
-
 boiling, 2 min: 100% loss of activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.68 6.8
-
-
 Rattus norvegicus