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Literature summary extracted from

  • Schär, H.P.; Holzmann, W.; Ramos Tombo, G.M.; Ghisalba, O.
    Purification and characterization of N,N-dimethylformamidase from Pseudomonas DMF 3/3 (1986), Eur. J. Biochem., 158, 469-475.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.56 2,2'-dipyridyl
-
 Pseudomonas sp.
3.5.1.56 EDTA
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.56 13.8
-
 N,N-Dimethylformamide
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.56 Fe iron protein Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.56 15000
-
 alpha2beta2, 2 * 15000 + 2 * 105000, SDS-PAGE Pseudomonas sp.
3.5.1.56 105000
-
 alpha2beta2, 2 * 15000 + 2 * 105000, SDS-PAGE Pseudomonas sp.
3.5.1.56 250000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.56 N,N-dimethylformamide + H2O Pseudomonas sp.
-
 dimethylamine + formate
-
 ?
3.5.1.56 N,N-dimethylformamide + H2O Pseudomonas sp. DMF 3/3
-
 dimethylamine + formate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.56 Pseudomonas sp.
-
-
-
3.5.1.56 Pseudomonas sp. DMF 3/3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.56
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.56 1.98
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.56 formamide + H2O 3.8% of the activity with N,N-dimethylformamide Pseudomonas sp. NH3 + formate
-
 ?
3.5.1.56 additional information less than 1% of the activity with N,N-dimethylformamide: acetamide, acrylamide, butyramide Pseudomonas sp. ?
-
 ?
3.5.1.56 additional information less than 1% of the activity with N,N-dimethylformamide: acetamide, acrylamide, butyramide Pseudomonas sp. DMF 3/3 ?
-
 ?
3.5.1.56 N,N-diethylformamide + H2O 2.6% of the activity with N,N-dimethylformamide Pseudomonas sp. diethylamine + formate
-
 ?
3.5.1.56 N,N-diethylformamide + H2O 2.6% of the activity with N,N-dimethylformamide Pseudomonas sp. DMF 3/3 diethylamine + formate
-
 ?
3.5.1.56 N,N-dimethylacetamide + H2O 3.4% of the activity with N,N-dimethylformamide Pseudomonas sp. dimethylamine + acetate
-
 ?
3.5.1.56 N,N-dimethylformamide + H2O
-
 Pseudomonas sp. dimethylamine + formate
-
 ?
3.5.1.56 N,N-dimethylformamide + H2O
-
 Pseudomonas sp. DMF 3/3 dimethylamine + formate
-
 ?
3.5.1.56 N-ethylformamide + H2O 31.5% of the activity with N,N-dimethylformamide Pseudomonas sp. ethylamine + formate
-
 ?
3.5.1.56 N-ethylformamide + H2O 31.5% of the activity with N,N-dimethylformamide Pseudomonas sp. DMF 3/3 ethylamine + formate
-
 ?
3.5.1.56 N-methylformamide + H2O 14.8% of the activity with N,N-dimethylformamide Pseudomonas sp. methylamine + formate
-
 ?
3.5.1.56 N-methylformamide + H2O 14.8% of the activity with N,N-dimethylformamide Pseudomonas sp. DMF 3/3 methylamine + formate
-
 ?
3.5.1.56 prolinamide + H2O 1.6% of the activity with N,N-dimethylformamide Pseudomonas sp. NH3 + proline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.56 tetramer alpha2beta2, 2 * 15000 + 2 * 105000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.56 40
-
-
 Pseudomonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.56 20 80 20°C: 20% of activity maximum, 80°C: 10% of activity maximum Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.56 20
-
 optimal stability below Pseudomonas sp.
3.5.1.56 60
-
 30 min, complete loss of activity Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.56 5 6
-
 Pseudomonas sp.
3.5.1.56 7.5
-
 optimal pH for temperatures of 45°C and 50°C Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.56 3 9.5 pH 3: 72% of activity maximum, pH 9.5: 73% of activity maximum Pseudomonas sp.