BRENDA - Enzyme Database

Purification and properties of the allophanate hydrolase from Chlamydomonas reinhardii

Maitz, G.S.; Haas, E.M.; Castric, P.A.; Biochim. Biophys. Acta 714, 486-491 (1982)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.54
5,5'-dithiobis(2-nitrobenzoic acid)
-
Chlamydomonas reinhardtii
3.5.1.54
acetoacetic acid
Ki 0.3 mM
Chlamydomonas reinhardtii
3.5.1.54
malonic acid
-
Chlamydomonas reinhardtii
3.5.1.54
NEM
allophanate protects
Chlamydomonas reinhardtii
3.5.1.54
oxaloacetic acid
-
Chlamydomonas reinhardtii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.54
0.55
-
allophanate
-
Chlamydomonas reinhardtii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.54
100000
-
gel filtration
Chlamydomonas reinhardtii
3.5.1.54
110000
-
PAGE
Chlamydomonas reinhardtii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.54
allophanate + H2O
Chlamydomonas reinhardtii
2nd step in the degradation of urea
CO2 + NH3
-
-
ir
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.54
Chlamydomonas reinhardtii
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.54
-
Chlamydomonas reinhardtii
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.54
19.3
-
-
Chlamydomonas reinhardtii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.54
allophanate + H2O
-
209196
Chlamydomonas reinhardtii
CO2 + NH3
-
-
-
ir
3.5.1.54
allophanate + H2O
2nd step in the degradation of urea
209196
Chlamydomonas reinhardtii
CO2 + NH3
-
-
-
ir
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.54
9
-
-
Chlamydomonas reinhardtii
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.54
7
10
pH 7.0: about 65% of maximal activity, pH 10.0: about 30% of maximal activity
Chlamydomonas reinhardtii
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.1.54
0.3
-
acetoacetic acid
-
Chlamydomonas reinhardtii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.54
5,5'-dithiobis(2-nitrobenzoic acid)
-
Chlamydomonas reinhardtii
3.5.1.54
acetoacetic acid
Ki 0.3 mM
Chlamydomonas reinhardtii
3.5.1.54
malonic acid
-
Chlamydomonas reinhardtii
3.5.1.54
NEM
allophanate protects
Chlamydomonas reinhardtii
3.5.1.54
oxaloacetic acid
-
Chlamydomonas reinhardtii
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.1.54
0.3
-
acetoacetic acid
-
Chlamydomonas reinhardtii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.54
0.55
-
allophanate
-
Chlamydomonas reinhardtii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.54
100000
-
gel filtration
Chlamydomonas reinhardtii
3.5.1.54
110000
-
PAGE
Chlamydomonas reinhardtii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.54
allophanate + H2O
Chlamydomonas reinhardtii
2nd step in the degradation of urea
CO2 + NH3
-
-
ir
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.54
-
Chlamydomonas reinhardtii
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.54
19.3
-
-
Chlamydomonas reinhardtii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.54
allophanate + H2O
-
209196
Chlamydomonas reinhardtii
CO2 + NH3
-
-
-
ir
3.5.1.54
allophanate + H2O
2nd step in the degradation of urea
209196
Chlamydomonas reinhardtii
CO2 + NH3
-
-
-
ir
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.54
9
-
-
Chlamydomonas reinhardtii
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.54
7
10
pH 7.0: about 65% of maximal activity, pH 10.0: about 30% of maximal activity
Chlamydomonas reinhardtii