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Literature summary extracted from

  • Greenwood, J.A.; Mills, J.; Tyler, P.D.; Jones, C.W.
    Physiological regulation, purification and properties of urease from Methylophilus methylotrophus (1998), FEMS Microbiol. Lett., 160, 131-135.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.5 3.8
-
 Urea
-
 Methylophilus methylotrophus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.5 cytoplasm
-
 Methylophilus methylotrophus 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.5 15000
-
 alpha2beta2gamma2, 2 * 64000 + 2 * 15500 + 2 * 15000, SDS-PAGE Methylophilus methylotrophus
3.5.1.5 15500
-
 alpha2beta2gamma2, 2 * 64000 + 2 * 15500 + 2 * 15000, SDS-PAGE Methylophilus methylotrophus
3.5.1.5 64000
-
 alpha2beta2gamma2, 2 * 64000 + 2 * 15500 + 2 * 15000, SDS-PAGE Methylophilus methylotrophus
3.5.1.5 190000
-
 gel filtration Methylophilus methylotrophus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.5 urea + H2O Methylophilus methylotrophus enzyme is induced by urea and short-chain amides, repressed by excess ammonia ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.5 Methylophilus methylotrophus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.5
-
 Methylophilus methylotrophus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.5 urea + H2O
-
 Methylophilus methylotrophus CO2 + NH3
-
 ?
3.5.1.5 urea + H2O enzyme is induced by urea and short-chain amides, repressed by excess ammonia Methylophilus methylotrophus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.5 hexamer alpha2beta2gamma2, 2 * 64000 + 2 * 15500 + 2 * 15000, SDS-PAGE Methylophilus methylotrophus