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Literature summary extracted from

  • Park, I.S.; Hausinger, R.P.
    Site-directed mutagenesis of Klebsiella aerogenes urease: identification of histidine residues that appear to function in nickel ligation, substrate binding and catalysis (1993), Protein Sci., 2, 1034-1041.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.5 alphaH134A no detectable activity, 53% of the nickel content of wild-type enzyme Klebsiella aerogenes
3.5.1.5 alphaH136A no detectable activity, 6% of the nickel content of wild-type enzyme Klebsiella aerogenes
3.5.1.5 alphaH219A 1.9% of the activity of the wild-type enzyme, 80% of the nickel content of the wild type enzyme, very high Km-value compared to wild-type enzyme Klebsiella aerogenes
3.5.1.5 alphaH246A no detectable activity, 21% of the nickel content of wild-type enzyme Klebsiella aerogenes
3.5.1.5 alphaH320A normal nickel content, 0.003% of the activity of the wild-type enzyme, unlike wild-type enzyme no inactivation by diethyldicarbonate Klebsiella aerogenes
3.5.1.5 alphaH321A activity and nickel content are similar to wild-type enzyme Klebsiella aerogenes
3.5.1.5 betaH39A activity and nickel content are similar to wild-type enzyme Klebsiella aerogenes
3.5.1.5 betaH41A activity and nickel content are similar to wild-type enzyme Klebsiella aerogenes
3.5.1.5 gammaH96A activity and nickel content are similar to wild-type enzyme Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.5 1.4
-
 Urea mutant betaH41A Klebsiella aerogenes
3.5.1.5 1.5
-
 Urea mutant betaH39A Klebsiella aerogenes
3.5.1.5 1.6
-
 Urea mutant alphah312A Klebsiella aerogenes
3.5.1.5 1.9
-
 Urea mutant gammaH96A Klebsiella aerogenes
3.5.1.5 2
-
 Urea mutant alphaH321A Klebsiella aerogenes
3.5.1.5 2.3
-
 Urea wild-type enzyme Klebsiella aerogenes
3.5.1.5 8.3
-
 Urea mutant alphaH320A Klebsiella aerogenes
3.5.1.5 1100
-
 Urea mutant alphaH219A Klebsiella aerogenes

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.5 Klebsiella aerogenes
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.5 urea + H2O
-
 Klebsiella aerogenes CO2 + NH3
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.5 50
-
 2 h, 30% loss of activity, wild-type enzyme Klebsiella aerogenes
3.5.1.5 60
-
 2 h, 55% loss of activity, wild-type enzyme. 2 h, complete inactivation of mutant enzymes betaH39A and alphaH320A Klebsiella aerogenes
3.5.1.5 70
-
 1 h, 87% loss of activity, wild-type enzyme, 58% loss of activity of mutant enzyme alphaG312A, complete inactivation of mutant enzymes gammaH96A, betaH39A, betaH41A, alphaH219A, alphaH320A and alphaH321A Klebsiella aerogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.5 6.8
-
 mutant enzyme alphaH320A Klebsiella aerogenes
3.5.1.5 7.6
-
 wild-type enzyme Klebsiella aerogenes