Literature summary extracted from

Lee, M.H.; Mulrooney, S.B.; Hausinger, R.P.
Purification, characterization, and in vivo reconstitution of Klebsiella aerogenes urease apoenzyme (1990), J. Bacteriol., 172, 4427-4431.

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
3.5.1.5	Nickel	addition of Ni to purified apourease does not yield active enzyme, the apoenzyme is very slowly activated in vivo by addition of Ni2+ ions to Ni-free cell cultures, apourease activation is an energy-dependent process that is deactivated by cell disruption	Klebsiella aerogenes
3.5.1.5	Nickel	nickel metalloenzyme	Klebsiella aerogenes

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.5.1.5	9000	-	alpha2beta4tau4, 2 * 72000 + 4 * 11000 + 4 * 9000, SDS-PAGE	Klebsiella aerogenes
3.5.1.5	11000	-	alpha2beta4tau4, 2 * 72000 + 4 * 11000 + 4 * 9000, SDS-PAGE	Klebsiella aerogenes
3.5.1.5	72000	-	alpha2beta4tau4, 2 * 72000 + 4 * 11000 + 4 * 9000, SDS-PAGE	Klebsiella aerogenes
3.5.1.5	224000	-	gel filtration	Klebsiella aerogenes

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.5.1.5	Klebsiella aerogenes	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.5.1.5	apoenzyme	Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.5.1.5	urea + H2O	-	Klebsiella aerogenes	CO2 + NH3	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.5.1.5	decamer	alpha2beta4tau4, 2 * 72000 + 4 * 11000 + 4 * 9000, SDS-PAGE	Klebsiella aerogenes

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
3.5.1.5	additional information	-	Ni substantially increase stability of the intact metalloprotein, Tm = 79°C compared with apoenzyme Tm = 62°C	Klebsiella aerogenes

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Release 2023.1 (January 2023)