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Literature summary extracted from

  • Schaefer, U.K.; Kaltwasser, H.
    Urease from Staphylococcus saprophyticus: purification, characterization and comparison to Staphylococcus xylosus urease (1994), Arch. Microbiol., 161, 393-399.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.5 Acetohydroxamate
-
 Staphylococcus saprophyticus
3.5.1.5 Phenylurea weak non-competitive Staphylococcus saprophyticus
3.5.1.5 Semicarbazide competitive Staphylococcus saprophyticus
3.5.1.5 Urea competitive Staphylococcus saprophyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.5 9.5
-
 Urea
-
 Staphylococcus saprophyticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.5 Nickel enzyme contains 4.09 atoms of nickel per molecule Staphylococcus saprophyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.5 13900
-
 alpha4beta4gamma4, 4 * 72400 + 4 * 20400, + 4 * 13900, SDS-PAGE Staphylococcus saprophyticus
3.5.1.5 20400
-
 alpha4beta4gamma4, 4 * 72400 + 4 * 20400, + 4 * 13900, SDS-PAGE Staphylococcus saprophyticus
3.5.1.5 72400
-
 alpha4beta4gamma4, 4 * 72400 + 4 * 20400, + 4 * 13900, SDS-PAGE Staphylococcus saprophyticus
3.5.1.5 410000
-
 non-denaturing PAGE Staphylococcus xylosus
3.5.1.5 420000
-
 non-denaturing PAGE Staphylococcus saprophyticus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.5 Staphylococcus saprophyticus
-
-
-
3.5.1.5 Staphylococcus xylosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.5
-
 Staphylococcus saprophyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.5 1979
-
-
 Staphylococcus saprophyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.5 urea + H2O
-
 Staphylococcus saprophyticus CO2 + NH3
-
 ?
3.5.1.5 urea + H2O
-
 Staphylococcus xylosus CO2 + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.5 dodecamer alpha4beta4gamma4, 4 * 72400 + 4 * 20400, + 4 * 13900, SDS-PAGE Staphylococcus saprophyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.5 55
-
-
 Staphylococcus saprophyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.5 6
-
 in citrate buffer Staphylococcus saprophyticus
3.5.1.5 7
-
 in citrate buffer Staphylococcus saprophyticus