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Literature summary extracted from

  • del Mar Dobao, M.; Castillo, F.; Pineda, M.
    Characterization of urease from the phototrophic bacterium Rhodobacter capsulatus E1F1 (1993), Curr. Microbiol., 27, 119-123.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.5 1,10-phenanthroline 5 mM, 67% inhibition Rhodobacter capsulatus
3.5.1.5 cyanide 1.0 mM, 32% inhibition Rhodobacter capsulatus
3.5.1.5 EDTA 5 mM, 70% inhibition Rhodobacter capsulatus
3.5.1.5 hydroxamic acid 1.0 mM, 64% inhibition Rhodobacter capsulatus
3.5.1.5 p-hydroxymercuribenzoate 0.5 mM, 90% inhibition Rhodobacter capsulatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.5 0.28
-
 Urea
-
 Rhodobacter capsulatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.5 cytosol
-
 Rhodobacter capsulatus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.5 180000
-
 sucrose density gradient centrifugation Rhodobacter capsulatus
3.5.1.5 185000
-
 gel filtration Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.5 urea + H2O Rhodobacter capsulatus NH4+ inhibits urease expression ?
-
 ?
3.5.1.5 urea + H2O Rhodobacter capsulatus E1F1 NH4+ inhibits urease expression ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.5 Rhodobacter capsulatus
-
-
-
3.5.1.5 Rhodobacter capsulatus E1F1
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.5 8
-
-
 Rhodobacter capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.5 urea + H2O
-
 Rhodobacter capsulatus CO2 + NH3
-
 ?
3.5.1.5 urea + H2O
-
 Rhodobacter capsulatus E1F1 CO2 + NH3
-
 ?
3.5.1.5 urea + H2O NH4+ inhibits urease expression Rhodobacter capsulatus ?
-
 ?
3.5.1.5 urea + H2O NH4+ inhibits urease expression Rhodobacter capsulatus E1F1 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.5 40
-
-
 Rhodobacter capsulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.5 9
-
-
 Rhodobacter capsulatus