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Literature summary extracted from

  • Lee, S.G.; Calhoun, D.H.
    Urease from a potentially pathogenic coccoid isolate: purification, characzterization, and comparison to other microbial ureases (1997), Infect. Immun., 65, 3991-3996.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.5.1.5 unstable during purification in absence of nickel. When 0.005 mM NiSO4 is present during growth and at 0.1 mM in buffers during sonication and purification, the urease is completely stable at room temperature during the purification procedure bacterium strain SL100

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.5 1.7
-
 Urea
-
 bacterium strain SL100

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.5 Nickel nickel metalloenzyme bacterium strain SL100

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.5 21000
-
 3 * 21000 + 3 * 65000, SDS-PAGE bacterium strain SL100
3.5.1.5 65000
-
 3 * 21000 + 3 * 65000, SDS-PAGE bacterium strain SL100
3.5.1.5 260000
-
 gel filtration bacterium strain SL100

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.5 urea + H2O bacterium strain SL100 urease may be a virulence factor during colonization of the stomach ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.5 bacterium strain SL100
-
 gram-positive coccoid isolate prototype with an adhesin specific for gastric mucin
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.5
-
 bacterium strain SL100

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.5 1120
-
-
 bacterium strain SL100

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.5 urea + H2O
-
 bacterium strain SL100 CO2 + NH3
-
 ?
3.5.1.5 urea + H2O urease may be a virulence factor during colonization of the stomach bacterium strain SL100 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.5 hexamer 3 * 21000 + 3 * 65000, SDS-PAGE bacterium strain SL100

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.5 1.3
-
 30 min, retains most of its activity bacterium strain SL100