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Literature summary extracted from

  • Nakano, H.; Takenishi, S.; Watanabe, Y.
    Purification and properties of urease from Brevibacterium ammoniagenes (1984), Agric. Biol. Chem., 48, 1495-1502.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.5 Co2+ 1 mM, 39% loss of activity Corynebacterium ammoniagenes
3.5.1.5 Cu2+ 1 mM, complete inactivation Corynebacterium ammoniagenes
3.5.1.5 Fe2+ 1 mM, 42% loss of activity Corynebacterium ammoniagenes
3.5.1.5 Hg2+ 1 mM, complete inactivation Corynebacterium ammoniagenes
3.5.1.5 Hydroxyurea
-
 Corynebacterium ammoniagenes
3.5.1.5 Ni2+ 1 mM, 26% loss of activity Corynebacterium ammoniagenes
3.5.1.5 PCMB 0.1 mM, complete loss of activity Corynebacterium ammoniagenes
3.5.1.5 Zn2+ 1 mM, 36% loss of activity Corynebacterium ammoniagenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.5 18 72 Urea value depends on pH and type of buffer Corynebacterium ammoniagenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.5 Nickel contains 0.8 gatom of nickel per 67000 g of protein Corynebacterium ammoniagenes
3.5.1.5 Nickel nickel metalloenzyme Corynebacterium ammoniagenes
3.5.1.5 Nickel contains 1 atom of nickel per subunit Corynebacterium ammoniagenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.5 67000
-
 x * 67000, SDS-PAGE Corynebacterium ammoniagenes
3.5.1.5 67000
-
 3 * 67000, SDS-PAGE Corynebacterium ammoniagenes
3.5.1.5 215000
-
 gel filtration Corynebacterium ammoniagenes

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.5 Corynebacterium ammoniagenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.5
-
 Corynebacterium ammoniagenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.5 3500
-
-
 Corynebacterium ammoniagenes

Storage Stability

EC Number Storage Stability Organism
3.5.1.5 20°C, 50 mM phosphate buffer, pH 7.5, 50% glycerol, 1 mM EDTA, 5 mM 2-mercaptoethanol, about 30% loss of activity after 2 months Corynebacterium ammoniagenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.5 urea + H2O
-
 Corynebacterium ammoniagenes CO2 + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.5 ? x * 67000, SDS-PAGE Corynebacterium ammoniagenes
3.5.1.5 trimer 3 * 67000, SDS-PAGE Corynebacterium ammoniagenes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.5 60 65
-
 Corynebacterium ammoniagenes

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.5 30 70 30°C: about 40% of maximal activity, 70°C: about 10% of maximal activity Corynebacterium ammoniagenes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.5 50
-
 pH 7.5, 10 min, stable below Corynebacterium ammoniagenes
3.5.1.5 60
-
 pH 7.5, 10 min, 50% loss of activity Corynebacterium ammoniagenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.5 7
-
-
 Corynebacterium ammoniagenes

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.5 7 10 30°C, 5 h, stable Corynebacterium ammoniagenes