BRENDA - Enzyme Database

A novel amidase, half-amidase, for half-amide hydrolysis involved in the metabolism of cyclic imides

Soong, C.L.; Ogawa, J.; Shimizu, S.; Appl. Environ. Microbiol. 66, 1947-1952 (2000)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
3.5.1.4
synthesis
-
Blastobacter sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.4
Ag+
inactivates enzyme activity by 76%
Blastobacter sp.
3.5.1.4
Cd2+
inactivates enzyme activity by 79%
Blastobacter sp.
3.5.1.4
Cu2+
inactivates enzyme activity by 90%
Blastobacter sp.
3.5.1.4
Hg2+
inactivates enzyme activity by 99%
Blastobacter sp.
3.5.1.4
succinate
-
Blastobacter sp.
3.5.1.4
sulfhydyl reagents
-
Blastobacter sp.
3.5.1.4
Zn2+
inactivates enzyme activity by 88%
Blastobacter sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.4
2.8
-
glutaramic acid
-
Blastobacter sp.
3.5.1.4
6.2
-
succinamic acid
-
Blastobacter sp.
3.5.1.4
6.5
-
Benzamide
-
Blastobacter sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.4
46000
-
1 * 48000, gel filtration and 1 * 46000, SDS-PAGE
Blastobacter sp.
3.5.1.4
48000
-
1 * 48000, gel filtration and 1 * 46000, SDS-PAGE
Blastobacter sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.4
benzamide
Blastobacter sp.
-
benzoic acid + NH3
-
Blastobacter sp.
?
3.5.1.4
glutaramic acid + H2O
Blastobacter sp.
-
glutaric acid + NH3
-
Blastobacter sp.
?
3.5.1.4
monoamidated dicarboxylate + H2O
Blastobacter sp.
-
dicarboxylate + ammonia
-
Blastobacter sp.
?
3.5.1.4
succinamic acid + H2O
Blastobacter sp.
-
succinic acid + NH3
-
Blastobacter sp.
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.4
Blastobacter sp.
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.4
cell extract purified by DEAE-Sephacel column, concentrated by ultrafiltration, phenyl-Sepharose column, Sephacryl S-200 column, Mono Q HR 5/5 and Mono Q PC 1.6/5 column
Blastobacter sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.4
benzamide
-
209109
Blastobacter sp.
benzoic acid + NH3
-
209109
Blastobacter sp.
?
3.5.1.4
glutaramic acid + H2O
-
209109
Blastobacter sp.
glutaric acid + NH3
-
209109
Blastobacter sp.
?
3.5.1.4
monoamidated dicarboxylate + H2O
-
209109
Blastobacter sp.
dicarboxylate + ammonia
-
209109
Blastobacter sp.
?
3.5.1.4
succinamic acid + H2O
-
209109
Blastobacter sp.
succinic acid + NH3
-
209109
Blastobacter sp.
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.1.4
monomer
1 * 48000, gel filtration and 1 * 46000, SDS-PAGE
Blastobacter sp.
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.5.1.4
35
-
-
Blastobacter sp.
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.1.4
0.00217
-
Benzamide
-
Blastobacter sp.
3.5.1.4
0.0372
-
glutaramic acid
-
Blastobacter sp.
3.5.1.4
0.096
-
succinamic acid
-
Blastobacter sp.
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.1.4
8
10
various buffer systems, 80% of the activity retained at 30C for 30 min
Blastobacter sp.
Application (protein specific)
EC Number
Application
Commentary
Organism
3.5.1.4
synthesis
-
Blastobacter sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.4
Ag+
inactivates enzyme activity by 76%
Blastobacter sp.
3.5.1.4
Cd2+
inactivates enzyme activity by 79%
Blastobacter sp.
3.5.1.4
Cu2+
inactivates enzyme activity by 90%
Blastobacter sp.
3.5.1.4
Hg2+
inactivates enzyme activity by 99%
Blastobacter sp.
3.5.1.4
succinate
-
Blastobacter sp.
3.5.1.4
sulfhydyl reagents
-
Blastobacter sp.
3.5.1.4
Zn2+
inactivates enzyme activity by 88%
Blastobacter sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.4
2.8
-
glutaramic acid
-
Blastobacter sp.
3.5.1.4
6.2
-
succinamic acid
-
Blastobacter sp.
3.5.1.4
6.5
-
Benzamide
-
Blastobacter sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.4
46000
-
1 * 48000, gel filtration and 1 * 46000, SDS-PAGE
Blastobacter sp.
3.5.1.4
48000
-
1 * 48000, gel filtration and 1 * 46000, SDS-PAGE
Blastobacter sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.4
benzamide
Blastobacter sp.
-
benzoic acid + NH3
-
Blastobacter sp.
?
3.5.1.4
glutaramic acid + H2O
Blastobacter sp.
-
glutaric acid + NH3
-
Blastobacter sp.
?
3.5.1.4
monoamidated dicarboxylate + H2O
Blastobacter sp.
-
dicarboxylate + ammonia
-
Blastobacter sp.
?
3.5.1.4
succinamic acid + H2O
Blastobacter sp.
-
succinic acid + NH3
-
Blastobacter sp.
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.4
cell extract purified by DEAE-Sephacel column, concentrated by ultrafiltration, phenyl-Sepharose column, Sephacryl S-200 column, Mono Q HR 5/5 and Mono Q PC 1.6/5 column
Blastobacter sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.4
benzamide
-
209109
Blastobacter sp.
benzoic acid + NH3
-
209109
Blastobacter sp.
?
3.5.1.4
glutaramic acid + H2O
-
209109
Blastobacter sp.
glutaric acid + NH3
-
209109
Blastobacter sp.
?
3.5.1.4
monoamidated dicarboxylate + H2O
-
209109
Blastobacter sp.
dicarboxylate + ammonia
-
209109
Blastobacter sp.
?
3.5.1.4
succinamic acid + H2O
-
209109
Blastobacter sp.
succinic acid + NH3
-
209109
Blastobacter sp.
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.1.4
monomer
1 * 48000, gel filtration and 1 * 46000, SDS-PAGE
Blastobacter sp.
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.5.1.4
35
-
-
Blastobacter sp.
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.1.4
0.00217
-
Benzamide
-
Blastobacter sp.
3.5.1.4
0.0372
-
glutaramic acid
-
Blastobacter sp.
3.5.1.4
0.096
-
succinamic acid
-
Blastobacter sp.
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.1.4
8
10
various buffer systems, 80% of the activity retained at 30C for 30 min
Blastobacter sp.