EC Number | Application | Comment | Organism |
---|---|---|---|
3.5.1.4 | synthesis | - |
Blastobacter sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.4 | Ag+ | inactivates enzyme activity by 76% | Blastobacter sp. | |
3.5.1.4 | Cd2+ | inactivates enzyme activity by 79% | Blastobacter sp. | |
3.5.1.4 | Cu2+ | inactivates enzyme activity by 90% | Blastobacter sp. | |
3.5.1.4 | Hg2+ | inactivates enzyme activity by 99% | Blastobacter sp. | |
3.5.1.4 | succinate | - |
Blastobacter sp. | |
3.5.1.4 | sulfhydyl reagents | - |
Blastobacter sp. | |
3.5.1.4 | Zn2+ | inactivates enzyme activity by 88% | Blastobacter sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.4 | 2.8 | - |
glutaramic acid | - |
Blastobacter sp. | |
3.5.1.4 | 6.2 | - |
succinamic acid | - |
Blastobacter sp. | |
3.5.1.4 | 6.5 | - |
Benzamide | - |
Blastobacter sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.1.4 | 46000 | - |
1 * 48000, gel filtration and 1 * 46000, SDS-PAGE | Blastobacter sp. |
3.5.1.4 | 48000 | - |
1 * 48000, gel filtration and 1 * 46000, SDS-PAGE | Blastobacter sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.4 | benzamide | Blastobacter sp. | - |
benzoic acid + NH3 | - |
? | |
3.5.1.4 | glutaramic acid + H2O | Blastobacter sp. | - |
glutaric acid + NH3 | - |
? | |
3.5.1.4 | monoamidated dicarboxylate + H2O | Blastobacter sp. | - |
dicarboxylate + ammonia | - |
? | |
3.5.1.4 | succinamic acid + H2O | Blastobacter sp. | - |
succinic acid + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.4 | Blastobacter sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.4 | cell extract purified by DEAE-Sephacel column, concentrated by ultrafiltration, phenyl-Sepharose column, Sephacryl S-200 column, Mono Q HR 5/5 and Mono Q PC 1.6/5 column | Blastobacter sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.4 | benzamide | - |
Blastobacter sp. | benzoic acid + NH3 | - |
? | |
3.5.1.4 | glutaramic acid + H2O | - |
Blastobacter sp. | glutaric acid + NH3 | - |
? | |
3.5.1.4 | monoamidated dicarboxylate + H2O | - |
Blastobacter sp. | dicarboxylate + ammonia | - |
? | |
3.5.1.4 | succinamic acid + H2O | - |
Blastobacter sp. | succinic acid + NH3 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.1.4 | monomer | 1 * 48000, gel filtration and 1 * 46000, SDS-PAGE | Blastobacter sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.4 | half-amidase | for half-amide hydrolysis | Blastobacter sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.4 | 35 | - |
- |
Blastobacter sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.4 | 0.00217 | - |
Benzamide | - |
Blastobacter sp. | |
3.5.1.4 | 0.0372 | - |
glutaramic acid | - |
Blastobacter sp. | |
3.5.1.4 | 0.096 | - |
succinamic acid | - |
Blastobacter sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.4 | 8 | 10 | various buffer systems, 80% of the activity retained at 30°C for 30 min | Blastobacter sp. |