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Literature summary extracted from

  • Nawaz, S.M.; Khan, A.A.; Seng J.E.; Leakey, J.E.; Siitonen, P.H.; Cerniglia, C.E.
    Purification and characterization of an amidase from an acrylamide-degrading Rhodococcus sp. (1994), Appl. Environ. Microbiol., 60, 3343-3348.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.1.4 synthesis
-
 Brevibacterium sp.
3.5.1.4 synthesis degradation of acrylamide Rhodococcus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.4 aromatic amide
-
 Rhodococcus sp.
3.5.1.4 Co2+
-
 Rhodococcus sp.
3.5.1.4 Cu2+
-
 Rhodococcus sp.
3.5.1.4 Fe2+ activity enhanced Rhodococcus sp.
3.5.1.4 Hg2+
-
 Rhodococcus sp.
3.5.1.4 iodoacetate
-
 Pseudomonas aeruginosa
3.5.1.4 iodoacetate
-
 Rhodococcus sp.
3.5.1.4 Mg2+ partially inhibited Rhodococcus sp.
3.5.1.4 Ni2+
-
 Rhodococcus sp.
3.5.1.4 specific iron chelator
-
 Rhodococcus sp.
3.5.1.4 thiol blocking reagent
-
 Rhodococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.4 1.2
-
 Acrylamide
-
 Rhodococcus sp.
3.5.1.4 2.6
-
 Propionamide
-
 Rhodococcus sp.
3.5.1.4 2.7
-
 acetamide
-
 Rhodococcus sp.
3.5.1.4 3.2
-
 butyramide
-
 Rhodococcus sp.
3.5.1.4 3.5
-
 Isobutyramide
-
 Rhodococcus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.4 Ba2+ activity enhanced Rhodococcus sp.
3.5.1.4 Cr2+ activity enhanced Rhodococcus sp.
3.5.1.4 Iron 8 mol of iron per mol of enzyme, detection by inductively coupled plasma-atomic emission spectrometry Rhodococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.4 26000
-
 x * 26000, SDS-PAGE Pseudomonas sp.
3.5.1.4 35000
-
 6 * 35000, SDS-PAGE Pseudomonas aeruginosa
3.5.1.4 43000
-
 gel filtration Pseudomonas sp.
3.5.1.4 43000
-
 4 * 43000 Brevibacterium sp.
3.5.1.4 44500
-
 8 * 44500, SDS-PAGE Rhodococcus sp.
3.5.1.4 180000
-
-
 Brevibacterium sp.
3.5.1.4 200000
-
 gel filtration and sedimentation equilibrium analysis, sedimentation coeffient 10.6 S Pseudomonas aeruginosa
3.5.1.4 360000
-
 PAGE, nondenaturing conditions Rhodococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.4 acrylamide + H2O Rhodococcus sp.
-
 acrylic acid + NH3
-
 ?
3.5.1.4 butyramide + H2O Rhodococcus sp.
-
 n-butyrate + NH3
-
 ?
3.5.1.4 isobutyramide + H2O Rhodococcus sp.
-
 isobutyrate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.4 Brevibacterium sp.
-
-
-
3.5.1.4 Pseudomonas aeruginosa
-
-
-
3.5.1.4 Pseudomonas sp.
-
-
-
3.5.1.4 Rhodococcus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.4 acetone precipitation, ammonium sulfate fractionation, DEAE-Sepharose chromatography, Mono Q column and gel filtration, purified approximately 138fold with a yield of 20% Rhodococcus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.4 37.6
-
 micromol of NH3 * min-1 * mg of protein-1 Pseudomonas sp.
3.5.1.4 274.1
-
 micromol of NH3 * min-1 * mg of protein-1 Brevibacterium sp.
3.5.1.4 3500
-
 micromol of NH3 * min-1 * mg of protein-1 Rhodococcus sp.
3.5.1.4 5000
-
 substrate isobutyramide Rhodococcus sp.
3.5.1.4 5900
-
 substrate acrylamide Rhodococcus sp.
3.5.1.4 7700
-
 substrate butyramide Rhodococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.4 acrylamide + H2O
-
 Rhodococcus sp. acrylic acid + NH3
-
 ?
3.5.1.4 butyramide + H2O
-
 Rhodococcus sp. n-butyrate + NH3
-
 ?
3.5.1.4 isobutyramide + H2O
-
 Rhodococcus sp. isobutyrate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.4 ? x * 26000, SDS-PAGE Pseudomonas sp.
3.5.1.4 hexamer 6 * 35000, SDS-PAGE Pseudomonas aeruginosa
3.5.1.4 octamer 8 * 44500, SDS-PAGE Rhodococcus sp.
3.5.1.4 tetramer 4 * 43000 Brevibacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.4 8.5
-
 at 40°C Rhodococcus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.4 5.5 9
-
 Rhodococcus sp.