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Literature summary extracted from

  • Holcenberg, J.S.; Teller, D.C.
    Physical properties of antitumor glutaminase-asparaginase from Pseudomonas 7A (1976), J. Biol. Chem., 251, 5375-5380.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.38 36000
-
 4 * 36000, sedimentation equilibrium analysis in the presence of guanidine HCl, amino acid analysis Pseudomonas sp.
3.5.1.38 140000
-
 sedimentation equilibrium analysis Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.38 L-asparagine + H2O Pseudomonas sp.
-
 L-aspartate + NH3
-
 ?
3.5.1.38 L-asparagine + H2O Pseudomonas sp. 7A
-
 L-aspartate + NH3
-
 ?
3.5.1.38 L-glutamine + H2O Pseudomonas sp. higher activity for glutaminase than for asparaginase L-glutamate + NH3
-
 ?
3.5.1.38 L-glutamine + H2O Pseudomonas sp. 7A higher activity for glutaminase than for asparaginase L-glutamate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.38 Pseudomonas sp.
-
-
-
3.5.1.38 Pseudomonas sp. 7A
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.38 to homogeneity Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.38 cell culture
-
 Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.38 100
-
 for L-asparagine Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.38 5-diazo-4-oxo-L-norvaline + H2O
-
 Pseudomonas sp. ?
-
 ?
3.5.1.38 5-diazo-4-oxo-L-norvaline + H2O
-
 Pseudomonas sp. 7A ?
-
 ?
3.5.1.38 L-asparagine + H2O
-
 Pseudomonas sp. L-aspartate + NH3
-
 ?
3.5.1.38 L-asparagine + H2O
-
 Pseudomonas sp. 7A L-aspartate + NH3
-
 ?
3.5.1.38 L-glutamine + H2O higher activity for glutaminase than for asparaginase Pseudomonas sp. L-glutamate + NH3
-
 ?
3.5.1.38 L-glutamine + H2O higher activity for glutaminase than for asparaginase Pseudomonas sp. 7A L-glutamate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.38 tetramer 4 * 36000, sedimentation equilibrium analysis in the presence of guanidine HCl, amino acid analysis Pseudomonas sp.