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Literature summary extracted from

  • Davidson, L.; Burkom, M.; Ahn, S.; Chang, L.C.; Kitto, B.
    L-Asparaginases from Citrobacter freundii (1977), Biochim. Biophys. Acta, 480, 282-294.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.38 p-chloromercuribenzoate complete inhibition at 0.1 mM, asparaginases A and B Citrobacter freundii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.38 0.029
-
 L-asparagine asparaginase A Citrobacter freundii
3.5.1.38 1.7
-
 L-asparagine asparaginase B Citrobacter freundii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.38 33000
-
 4 * 33000, SDS-PAGE Citrobacter freundii
3.5.1.38 140000
-
 asparaginase A, gel filtration Citrobacter freundii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.38 L-asparagine + H2O Citrobacter freundii
-
 L-aspartate + NH3
-
 ?
3.5.1.38 L-glutamine + H2O Citrobacter freundii low activity L-glutamate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.38 Citrobacter freundii
-
 3 forms: asparaginase-glutaminase, asparaginase A, asparaginase B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.38 to homogeneity, asparaginase A Citrobacter freundii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.38 cell culture
-
 Citrobacter freundii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.38 1.6
-
 asparaginase B Citrobacter freundii
3.5.1.38 133
-
 asparaginase A Citrobacter freundii

Storage Stability

EC Number Storage Stability Organism
3.5.1.38 4°C, 12 months, asparaginase A, asparaginase B: DTT required, 6 months Citrobacter freundii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.38 aspartic acid + H2O very low activity only for asparaginase B Citrobacter freundii ?
-
 ?
3.5.1.38 beta-D-aspartyl hydroxylammonium sulfate + H2O only asparaginase A, low activity Citrobacter freundii L-Asp + hydroxylammonium sulfate
-
 ?
3.5.1.38 beta-L-aspartyl hydroxylammonium sulfate + H2O higher activity for asparaginase B Citrobacter freundii L-Asp + hydroxylammonium sulfate
-
 ?
3.5.1.38 D-asparagine + H2O low activity Citrobacter freundii D-aspartate + NH3
-
 ?
3.5.1.38 D-glutamine + H2O low activity Citrobacter freundii D-glutamate + NH3
-
 ?
3.5.1.38 gamma-L-glutamyl hydroxylammonium sulfate + H2O very low activities for both asparaginases A and B Citrobacter freundii L-glutamate + hydroxylammonium sulfate
-
 ?
3.5.1.38 L-asparagine + H2O
-
 Citrobacter freundii L-aspartate + NH3
-
 ?
3.5.1.38 L-glutamine + H2O low activity Citrobacter freundii L-glutamate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.38 tetramer 4 * 33000, SDS-PAGE Citrobacter freundii

Synonyms

EC Number Synonyms Comment Organism
3.5.1.38 asparaginase A
-
 Citrobacter freundii
3.5.1.38 asparaginase B
-
 Citrobacter freundii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.38 51
-
 asparaginase B, half-life: 10 min Citrobacter freundii