EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.38 | p-chloromercuribenzoate | 26% inhibition at 0.1 mM | Delftia acidovorans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.38 | 0.015 | - |
L-asparagine | - |
Delftia acidovorans | |
3.5.1.38 | 0.022 | - |
L-glutamine | - |
Delftia acidovorans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.1.38 | 39000 | - |
4 * 39000, SDS-PAGE | Delftia acidovorans |
3.5.1.38 | 156000 | - |
gel filtration | Delftia acidovorans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.38 | L-asparagine + H2O | Delftia acidovorans | hydrolysed at a rate of 0.83 compared to L-glutamine | L-aspartate + NH3 | - |
? | |
3.5.1.38 | L-glutamine + H2O | Delftia acidovorans | - |
L-glutamate + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.38 | Delftia acidovorans | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.38 | to homogeneity, 3step chromatography | Delftia acidovorans |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.5.1.38 | cell culture | - |
Delftia acidovorans | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.1.38 | 86 | - |
asparaginase activity | Delftia acidovorans |
3.5.1.38 | 104 | - |
glutaminase activity | Delftia acidovorans |
EC Number | Storage Stability | Organism |
---|---|---|
3.5.1.38 | 4°C, 50 mM borate buffer, pH 7.0, 100 mM NaCl, 1 mM EDTA, 3 to 4 months | Delftia acidovorans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.38 | beta-D-aspartyl hydroxylammonium sulfate + H2O | - |
Delftia acidovorans | L-Asp + hydroxylammonium sulfate | - |
? | |
3.5.1.38 | beta-L-aspartyl hydroxylammonium sulfate + H2O | - |
Delftia acidovorans | L-Asp + hydroxylammonium sulfate | - |
? | |
3.5.1.38 | D-asparagine + H2O | low activity | Delftia acidovorans | D-aspartate + NH3 | - |
? | |
3.5.1.38 | D-glutamine + H2O | low activity | Delftia acidovorans | D-glutamate + NH3 | - |
? | |
3.5.1.38 | gamma-L-glutamyl hydroxylammonium sulfate + H2O | - |
Delftia acidovorans | L-glutamate + hydroxylammonium sulfate | - |
? | |
3.5.1.38 | L-asparagine + H2O | hydrolysed at a rate of 0.83 compared to L-glutamine | Delftia acidovorans | L-aspartate + NH3 | - |
? | |
3.5.1.38 | L-glutamine + H2O | - |
Delftia acidovorans | L-glutamate + NH3 | - |
? | |
3.5.1.38 | N-acetyl-L-asparagine + H2O | 5% of the activity compared to L-asparagine | Delftia acidovorans | N-acetyl-L-aspartate + NH3 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.1.38 | tetramer | 4 * 39000, SDS-PAGE | Delftia acidovorans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.38 | 50 | - |
half-life: 10 min, protection against thermal inactivation by addition of substrate | Delftia acidovorans |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.38 | 7.4 | - |
70% activity | Delftia acidovorans |