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Literature summary extracted from

  • Huser, A.; Klöppner, U.; Röhm, K.H.
    Cloning, sequence analysis, and expression of ansB from Pseudomonas fluorescens, encoding periplasmic glutaminase/asparaginase (1999), FEMS Microbiol. Lett., 178, 327-335.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.38 expression in Escherichia coli Pseudomonas fluorescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.38 periplasm
-
 Pseudomonas fluorescens
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.38 L-asparagine + H2O Pseudomonas fluorescens
-
 L-aspartate + NH3
-
 ?
3.5.1.38 L-glutamine + H2O Pseudomonas fluorescens
-
 L-glutamate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.38 Pseudomonas fluorescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.38 near homogeneity, recombinant enzyme Pseudomonas fluorescens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.38 cell culture
-
 Pseudomonas fluorescens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.38 beta-L-aspartyl hydroxylammonium sulfate + H2O
-
 Pseudomonas fluorescens L-Asp + hydroxylammonium sulfate
-
 ?
3.5.1.38 D-asparagine + H2O
-
 Pseudomonas fluorescens D-aspartate + NH3
-
 ?
3.5.1.38 L-asparagine + H2O
-
 Pseudomonas fluorescens L-aspartate + NH3
-
 ?
3.5.1.38 L-glutamine + H2O
-
 Pseudomonas fluorescens L-glutamate + NH3
-
 ?