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Literature summary extracted from

  • Ortlund, E.; Lacount, M.W.; Lewinski, K.; Lebioda, L.
    Reactions of Pseudomonas 7A glutaminase-asparaginase with diazo analogues of glutamine and asparagine result in unexpected covalent inhibitions and suggests an unusual catalytic triad Thr-Tyr-Glu (2000), Biochemistry, 39, 1199-1204.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.38 hanging drop vapor diffusion method, in presence of inhibitors Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.38 5-Diazo-4-oxo-L-norvaline inactivation by covalent binding to Thr20 and Tyr34 Pseudomonas sp.
3.5.1.38 6-diazo-5-oxo-L-norleucine inactivation by covalent binding to Thr20 and Tyr34 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.38 L-asparagine + H2O Pseudomonas sp.
-
 L-aspartate + NH3
-
 ?
3.5.1.38 L-asparagine + H2O Pseudomonas sp. 7A
-
 L-aspartate + NH3
-
 ?
3.5.1.38 L-glutamine + H2O Pseudomonas sp.
-
 L-glutamate + NH3
-
 ?
3.5.1.38 L-glutamine + H2O Pseudomonas sp. 7A
-
 L-glutamate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.38 Pseudomonas sp. P10182
-
-
3.5.1.38 Pseudomonas sp. 7A P10182
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.38 cell culture
-
 Pseudomonas sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.38 D-asparagine + H2O
-
 Pseudomonas sp. D-aspartate + NH3
-
 ?
3.5.1.38 D-asparagine + H2O
-
 Pseudomonas sp. 7A D-aspartate + NH3
-
 ?
3.5.1.38 D-glutamine + H2O
-
 Pseudomonas sp. D-glutamate + NH3
-
 ?
3.5.1.38 D-glutamine + H2O
-
 Pseudomonas sp. 7A D-glutamate + NH3
-
 ?
3.5.1.38 L-asparagine + H2O
-
 Pseudomonas sp. L-aspartate + NH3
-
 ?
3.5.1.38 L-asparagine + H2O
-
 Pseudomonas sp. 7A L-aspartate + NH3
-
 ?
3.5.1.38 L-glutamine + H2O
-
 Pseudomonas sp. L-glutamate + NH3
-
 ?
3.5.1.38 L-glutamine + H2O
-
 Pseudomonas sp. 7A L-glutamate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.38 tetramer native crystallization Pseudomonas sp.