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Literature summary extracted from

  • Kohno, M.; Yamashina, I.
    Purification and properties of 4-L-aspartylglycosylamine amidohydrolase from hog kidney (1972), Biochim. Biophys. Acta, 258, 600-617.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.5.1.26 stabilized by its substrate Asn-GlcNAc Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.26 aspartic acid 40% inhibition at 1 mM Sus scrofa
3.5.1.26 Cu2+
-
 Sus scrofa
3.5.1.26 EDTA 50% inhibition at 1 mM Sus scrofa
3.5.1.26 Mn2+
-
 Sus scrofa
3.5.1.26 Ni2+
-
 Sus scrofa
3.5.1.26 Zn2+
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.26 0.4
-
 2-acetamido-1-N-(4-L-aspartyl)-2-deoxy-beta-D-glycopyranosylamine for amidase-3 Sus scrofa
3.5.1.26 0.64
-
 2-acetamido-1-N-(4-L-aspartyl)-2-deoxy-beta-D-glycopyranosylamine amidase-1 and amidase-2, value depending on enzyme concentration Sus scrofa
3.5.1.26 0.77
-
 ovalbumin
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.26 70000
-
 disc electrophoresis, ultracentrifugation Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.26 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.26 to homogeneity, chromatography techniques Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.26 kidney 3 forms Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.26 1.55
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
3.5.1.26 -20°C, 0.02 M phosphate buffer, pH 7.0, invariably stable Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.26 2-acetamido-1-beta-(L-aspartamido)-1,2-dideoxy-beta-D-glucose + H2O
-
 Sus scrofa 2-acetamido-1-amino-1,2-dideoxy-beta-D-glucose + L-aspartate additional product: NH3, in equimolar amounts of N-acetylglucosamine and L-aspartate ?
3.5.1.26 ovalbumin + H2O
-
 Sus scrofa L-aspartate + ?
-
 ?
3.5.1.26 Taka-amylase + H2O
-
 Sus scrofa L-aspartate + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.26 amidase-1
-
 Sus scrofa
3.5.1.26 amidase-2
-
 Sus scrofa
3.5.1.26 amidase-3
-
 Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.26 5.5
-
 sharp activity peak Sus scrofa
3.5.1.26 8
-
 considerable aciivity with a broad peak Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.26 6
-
 stable above Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.26 1
-
 EDTA
-
 Sus scrofa